1887

Abstract

The membrane-impermeable thiol blocker 5′5-dithiobis 2- nitrobenzoic acid (DTNB) blocked infectivity of human cytomegalovirus (CMV) although the virus still bound to cells. DTNB-treated CMV regained 65% of its infectivity after incubation with the disulfide bond-reducing agent dithiothreitol. These observations suggest that free thiol groups on CMV are required for infectivity and may participate in disulfide bond formation during virus entry.

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1999-11-01
2019-10-15
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References

  1. Abell, B. A. & Brown, D. T. ( 1993; ). Sindbis virus membrane fusion is mediated by reduction of glycoprotein disulfide bridges at the cell surface. Journal of Virology 67, 5496-5501 .
    [Google Scholar]
  2. Billstrom, M. A. & Britt, W. J. ( 1995; ). Postoligomerization folding of human cytomegalovirus glycoprotein B: identification of folding intermediates and importance of disulfide bonding. Journal of Virology 69, 7015-7022 .
    [Google Scholar]
  3. Buelt, M. K. & Bernlohr, D. A. ( 1990; ). Modification of the adipocyte lipid binding protein by sulfhydryl reagents and analysis of the fatty acid binding domain. Biochemistry 29, 7408-7413 .[CrossRef]
    [Google Scholar]
  4. Compton, T. (1995). Towards a definition of the HCMV entry pathway. Scandinavian Journal of Infectious Diseases – Supplementum 1995; 99:30-2 99: 29- 32.
  5. Compton, T. , Nepomuceno, R. R. & Nowlin, D. M. ( 1992; ). Human cytomegalovirus penetrates host cells by pH-independent fusion at the cell surface. Virology 191, 387-395.[CrossRef]
    [Google Scholar]
  6. Compton, T. , Nowlin, D. M. & Cooper, N. R. ( 1993; ). Initiation of human cytomegalovirus infection requires initial interaction with cell surface heparan sulfate. Virology 193, 834-841.[CrossRef]
    [Google Scholar]
  7. Freed, E. O. & Martin, M. A. ( 1995; ). The role of human immunodeficiency virus type 1 envelope glycoproteins in virus infection. Journal of Biological Chemistry 270, 23883-23886 .[CrossRef]
    [Google Scholar]
  8. Haywood, A. M. ( 1994; ). Virus receptors: binding, adhesion strengthening, and changes in viral structure. Journal of Virology 68, 1-5.
    [Google Scholar]
  9. Kari, B. & Gehrz, R. ( 1992; ). A human cytomegalovirus glycoprotein complex designated gC-II is a major heparin-binding component of the envelope. Journal of Virology 66, 1761-1764 .
    [Google Scholar]
  10. Kari, B. & Gehrz, R. ( 1993; ). Structure, composition and heparin binding properties of a human cytomegalovirus glycoprotein complex designated gC-II. Journal of General Virology 74, 255-264.[CrossRef]
    [Google Scholar]
  11. Keay, S. & Baldwin, B. ( 1992; ). The human fibroblast receptor for gp86 of human cytomegalovirus is a phosphorylated glycoprotein. Journal of Virology 66, 4834-4838 .
    [Google Scholar]
  12. Keay, S. , Merigan, T. C. & Rasmussen, L. ( 1989; ). Identification of cell surface receptors for the 86-kilodalton glycoprotein of human cytomegalovirus. Proceedings of the National Academy of Sciences, USA 86, 10100-10103 .[CrossRef]
    [Google Scholar]
  13. Lamb, R. A. ( 1993; ). Paramyxovirus fusion: a hypothesis for changes. Virology 197, 1-11.[CrossRef]
    [Google Scholar]
  14. Li, B. , Copp, L. , Castelhano, A. L. , Feng, R. , Stahl, M. , Yuan, Z. & Krantz, A. ( 1994; ). Inactivation of a cytosolic phospholipase A2 by thiol-modifying reagents: cysteine residues as potential targets of phospholipase A2. Biochemistry 33, 8594-8603 .[CrossRef]
    [Google Scholar]
  15. McClure, M. O. , Marsh, M. & Weiss, R. A. ( 1988; ). Human immunodeficiency virus infection of CD4-bearing cells occurs by a pH-independent mechanism. EMBO Journal 7, 513-518.
    [Google Scholar]
  16. Mirazimi, A. & Svensson, L. ( 1998; ). Carbohydrates facilitate correct disulfide bond formation and folding of rotavirus VP7. Journal of Virology 72, 3887-3892 .
    [Google Scholar]
  17. Mirazimi, A. , von Bonsdorff, C. H. & Svensson, L. ( 1996; ). Effect of brefeldin A on rotavirus assembly and oligosaccharide processing. Virology 217, 554-563.[CrossRef]
    [Google Scholar]
  18. Ploux, O. , Lei, Y. , Vatanen, K. & Liu, H. W. ( 1995; ). Mechanistic studies on CDP-6-deoxy-delta 3, 4-glucoseen reductase: the role of cysteine residues in catalysis as probed by chemical modification and site- directed mutagenesis. Biochemistry 34, 4159-4168 .[CrossRef]
    [Google Scholar]
  19. Ryser, H. J. , Levy, E. M. , Mandel, R. & DiSciullo, G. J. ( 1994; ). Inhibition of human immunodeficiency virus infection by agents that interfere with thiol–disulfide interchange upon virus–receptor interaction. Proceedings of the National Academy of Sciences, USA 91, 4559-4563 .[CrossRef]
    [Google Scholar]
  20. Smith, D. J. , Maggio, E. T. & Kenyon, G. L. ( 1975; ). Simple alkanethiol groups for temporary blocking of sulfhydryl groups of enzymes. Biochemistry 14, 766-771.[CrossRef]
    [Google Scholar]
  21. Soderberg, C. , Giugni, T. D. , Zaia, J. A. , Larsson, S. , Wahlberg, J. M. & Moller, E. ( 1993; ). CD13 (human aminopeptidase N) mediates human cytomegalovirus infection. Journal of Virology 67, 6576-6585 .
    [Google Scholar]
  22. Spaete, R. R. , Gehrz, R. C. & Landini, M. P. ( 1994; ). Human cytomegalovirus structural proteins. Journal of General Virology 75, 3287-3308 .[CrossRef]
    [Google Scholar]
  23. Spear, P. G. ( 1993; ). Entry of alphaherpesviruses into cells. Seminars in Virology 4, 167-180.[CrossRef]
    [Google Scholar]
  24. Tsao, Y. S. & Huang, L. ( 1986; ). Kinetic studies of Sendai virus-target membrane interactions: independent analysis of binding and fusion. Biochemistry 25, 3971-3976 .[CrossRef]
    [Google Scholar]
  25. White, J. , Kielian, M. & Helenius, A. ( 1983; ). Membrane fusion proteins of enveloped animal viruses. Quarterly Reviews of Biophysics 16, 151-195.[CrossRef]
    [Google Scholar]
  26. Wickham, T. J. , Mathias, P. , Cheresh, D. A. & Nemerow, G. R. ( 1993; ). Integrins alpha v beta 3 and alpha v beta 5 promote adenovirus internalization but not virus attachment. Cell 73, 309-319.[CrossRef]
    [Google Scholar]
  27. Williams, R. K. , Jiang, G. S. & Holmes, K. V. ( 1991; ). Receptor for mouse hepatitis virus is a member of the carcinoembryonic antigen family of glycoproteins. Proceedings of the National Academy of Sciences, USA 88, 5533-5536 .[CrossRef]
    [Google Scholar]
  28. Wright, J. F. , Kurosky, A. & Wasi, S. ( 1994; ). An endothelial cell-surface form of annexin II binds human cytomegalovirus. Biochemical and Biophysical Research Communications 198, 983-989.[CrossRef]
    [Google Scholar]
  29. Zhi, W. , Srere, P. A. & Evans, C. T. ( 1991; ). Conformational stability of pig citrate synthase and some active-site mutants. Biochemistry 30, 9281-9286 .[CrossRef]
    [Google Scholar]
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