1887

Abstract

The membrane-impermeable thiol blocker 5′5-dithiobis 2- nitrobenzoic acid (DTNB) blocked infectivity of human cytomegalovirus (CMV) although the virus still bound to cells. DTNB-treated CMV regained 65% of its infectivity after incubation with the disulfide bond-reducing agent dithiothreitol. These observations suggest that free thiol groups on CMV are required for infectivity and may participate in disulfide bond formation during virus entry.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-11-2861
1999-11-01
2020-07-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/11/0802861a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-11-2861&mimeType=html&fmt=ahah

References

  1. Abell B. A., Brown D. T.. 1993; Sindbis virus membrane fusion is mediated by reduction of glycoprotein disulfide bridges at the cell surface. Journal of Virology67:5496–5501
    [Google Scholar]
  2. Billstrom M. A., Britt W. J.. 1995; Postoligomerization folding of human cytomegalovirus glycoprotein B: identification of folding intermediates and importance of disulfide bonding. Journal of Virology69:7015–7022
    [Google Scholar]
  3. Buelt M. K., Bernlohr D. A.. 1990; Modification of the adipocyte lipid binding protein by sulfhydryl reagents and analysis of the fatty acid binding domain. Biochemistry29:7408–7413
    [Google Scholar]
  4. Compton T.. 1995; Towards a definition of the HCMV entry pathway. Scandinavian Journal of Infectious Diseases – Supplementum 1995;99:30–2 99: 29-32
    [Google Scholar]
  5. Compton T., Nepomuceno R. R., Nowlin D. M.. 1992; Human cytomegalovirus penetrates host cells by pH-independent fusion at the cell surface. Virology191:387–395
    [Google Scholar]
  6. Compton T., Nowlin D. M., Cooper N. R.. 1993; Initiation of human cytomegalovirus infection requires initial interaction with cell surface heparan sulfate. Virology193:834–841
    [Google Scholar]
  7. Freed E. O., Martin M. A.. 1995; The role of human immunodeficiency virus type 1 envelope glycoproteins in virus infection. Journal of Biological Chemistry270:23883–23886
    [Google Scholar]
  8. Haywood A. M.. 1994; Virus receptors: binding, adhesion strengthening, and changes in viral structure. Journal of Virology68:1–5
    [Google Scholar]
  9. Kari B., Gehrz R.. 1992; A human cytomegalovirus glycoprotein complex designated gC-II is a major heparin-binding component of the envelope. Journal of Virology66:1761–1764
    [Google Scholar]
  10. Kari B., Gehrz R.. 1993; Structure, composition and heparin binding properties of a human cytomegalovirus glycoprotein complex designated gC-II. Journal of General Virology74:255–264
    [Google Scholar]
  11. Keay S., Baldwin B.. 1992; The human fibroblast receptor for gp86 of human cytomegalovirus is a phosphorylated glycoprotein. Journal of Virology66:4834–4838
    [Google Scholar]
  12. Keay S., Merigan T. C., Rasmussen L.. 1989; Identification of cell surface receptors for the 86-kilodalton glycoprotein of human cytomegalovirus. Proceedings of the National Academy of Sciences USA86:10100–10103
    [Google Scholar]
  13. Lamb R. A.. 1993; Paramyxovirus fusion: a hypothesis for changes. Virology197:1–11
    [Google Scholar]
  14. Li B., Copp L., Castelhano A. L., Feng R., Stahl M., Yuan Z., Krantz A.. 1994; Inactivation of a cytosolic phospholipase A2 by thiol-modifying reagents: cysteine residues as potential targets of phospholipase A2. Biochemistry33:8594–8603
    [Google Scholar]
  15. McClure M. O., Marsh M., Weiss R. A.. 1988; Human immunodeficiency virus infection of CD4-bearing cells occurs by a pH-independent mechanism. EMBO Journal7:513–518
    [Google Scholar]
  16. Mirazimi A., Svensson L.. 1998; Carbohydrates facilitate correct disulfide bond formation and folding of rotavirus VP7. Journal of Virology72:3887–3892
    [Google Scholar]
  17. Mirazimi A., von Bonsdorff C. H., Svensson L.. 1996; Effect of brefeldin A on rotavirus assembly and oligosaccharide processing. Virology217:554–563
    [Google Scholar]
  18. Ploux O., Lei Y., Vatanen K., Liu H. W.. 1995; Mechanistic studies on CDP-6-deoxy-delta 3, 4-glucoseen reductase: the role of cysteine residues in catalysis as probed by chemical modification and site- directed mutagenesis. Biochemistry34:4159–4168
    [Google Scholar]
  19. Ryser H. J., Levy E. M., Mandel R., DiSciullo G. J.. 1994; Inhibition of human immunodeficiency virus infection by agents that interfere with thiol–disulfide interchange upon virus–receptor interaction. Proceedings of the National Academy of Sciences USA91:4559–4563
    [Google Scholar]
  20. Smith D. J., Maggio E. T., Kenyon G. L.. 1975; Simple alkanethiol groups for temporary blocking of sulfhydryl groups of enzymes. Biochemistry14:766–771
    [Google Scholar]
  21. Soderberg C., Giugni T. D., Zaia J. A., Larsson S., Wahlberg J. M., Moller E.. 1993; CD13 (human aminopeptidase N) mediates human cytomegalovirus infection. Journal of Virology67:6576–6585
    [Google Scholar]
  22. Spaete R. R., Gehrz R. C., Landini M. P.. 1994; Human cytomegalovirus structural proteins. Journal of General Virology75:3287–3308
    [Google Scholar]
  23. Spear P. G.. 1993; Entry of alphaherpesviruses into cells. Seminars in Virology4:167–180
    [Google Scholar]
  24. Tsao Y. S., Huang L.. 1986; Kinetic studies of Sendai virus-target membrane interactions: independent analysis of binding and fusion. Biochemistry25:3971–3976
    [Google Scholar]
  25. White J., Kielian M., Helenius A.. 1983; Membrane fusion proteins of enveloped animal viruses. Quarterly Reviews of Biophysics16:151–195
    [Google Scholar]
  26. Wickham T. J., Mathias P., Cheresh D. A., Nemerow G. R.. 1993; Integrins alpha v beta 3 and alpha v beta 5 promote adenovirus internalization but not virus attachment. Cell73:309–319
    [Google Scholar]
  27. Williams R. K., Jiang G. S., Holmes K. V.. 1991; Receptor for mouse hepatitis virus is a member of the carcinoembryonic antigen family of glycoproteins. Proceedings of the National Academy of Sciences USA88:5533–5536
    [Google Scholar]
  28. Wright J. F., Kurosky A., Wasi S.. 1994; An endothelial cell-surface form of annexin II binds human cytomegalovirus. Biochemical and Biophysical Research Communications198:983–989
    [Google Scholar]
  29. Zhi W., Srere P. A., Evans C. T.. 1991; Conformational stability of pig citrate synthase and some active-site mutants. Biochemistry30:9281–9286
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-11-2861
Loading
/content/journal/jgv/10.1099/0022-1317-80-11-2861
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error