1887

Abstract

Bovine herpesvirus type 5 (BHV-5) is the causative agent of a fatal meningo-encephalitis in calves and is closely related to BHV-1 which causes infectious bovine rhinotracheitis. The gene encoding BHV-5 glycoprotein gH was sequenced. A high degree of conservation was found between BHV-1 and BHV-5 deduced gH amino acid sequences (86·4%), which is also observed for all alphaherpesvirus gH sequences. Transcriptional analysis revealed a 3·1 kb mRNA as the specific gH transcript which was detected 2 h post-infection (p.i.). Twelve out of twenty-one MAbs directed against BHV-1 gH immunoprecipitated a 108–110 kDa glycoprotein, which was then designated BHV-5 gH. Synthesis and intracellular processing of BHV- 5 gH was analysed in infected MDBK cells using gH cross-reacting MAbs. Glycoprotein gH was expressed as a beta-gamma protein, detected by radioimmunoprecipitation as early as 3 h p.i. Glycosylation studies indicated that BHV-5 gH contains -linked carbohydrates which are essential for the recognition of the protein by the MAbs. This suggests that -linked glycans are involved in protein folding or are targets for the gH cross-reacting MAbs. Plaque- reduction neutralization assays showed that at least one BHV-1 gH antigenic domain is lacking in BHV-5 which may possibly relate to differences in virus tropism.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-11-2849
1999-11-01
2020-09-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/11/0802849a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-11-2849&mimeType=html&fmt=ahah

References

  1. Abdelmagid O. Y., Minocha H. C., Collins J. K., Chowdhury S. I.. 1995; Fine mapping of bovine herpesvirus 1 (BHV-1) glycoprotein D (gD) neutralising epitopes by type-specific monoclonal antibodies and sequence comparison with BHV- 5 gD. Virology206:242–253
    [Google Scholar]
  2. Babic N., Klupp B. G., Makoschey B., Karger A., Flamand A., Mettenleiter T. C.. 1996; Glycoprotein gH of pseudorabies virus is essential for penetration and propagation in cell culture and in the nervous system of mice. Journal of General Virology77:2277–2285
    [Google Scholar]
  3. Bagust T. J., Clark L.. 1972; Pathogenesis of meningo-encephalitis produced in calves by infectious bovine rhinotracheitis herpesvirus. Journal of Comparative Pathology82:375–383
    [Google Scholar]
  4. Baranowski E.. 1996; Identification et caract érisation des glycoprotéines gH, gE, gG et gp42 du bovine herpèsvirus 1. PhD thesis University of Li ège;
  5. Baranowski E., Dubuisson J., Pastoret P.-P., Thiry E.. 1993; Identification of a 108K, 93K, and 42K glycoproteins of bovine herpesvirus-1 by monoclonal antibodies. Archives of Virology113:97–111
    [Google Scholar]
  6. Baranowski E., Dubuisson J., van Drunen Littel-van den Hurk S., Babiuk L. A., Michel A., Pastoret P.-P., Thiry E.. 1995; Synthesis and processing of bovine herpesvirus-1 glycoprotein H. Virology206:651–654
    [Google Scholar]
  7. Baranowski E., Keil G., Lyaku J., Rijsewijk F. A., van Oirschot J. T., Pastoret P. P., Thiry E.. 1996; Structural and functional analysis of bovine herpesvirus 1 minor glycoproteins. Veterinary Microbiology53:91–101
    [Google Scholar]
  8. Baumann R. P., Yalamanchili V. R. R., O’Callaghan D. J.. 1989; Functional mapping and DNA sequence of an equine herpesvirus 1 origin of replication. Journal of Virology63:1275–1283
    [Google Scholar]
  9. Belknap E. B., Collins J. K., Ayers V. K., Schulteiss P. C.. 1994; Experimental infection of neonatal calves with neurovirulent bovine herpesvirus type 1.3. Veterinary Pathology31:358–365
    [Google Scholar]
  10. Bello L. J., Whitbeck J. C., Lawrence W. C.. 1992; Sequence and transcript analysis of the bovine herpesvirus 1 thymidine kinase locus. Virology189:407–414
    [Google Scholar]
  11. Breathnach R., Chambon R.. 1981; Organisation and expression of eucaryotic split genes coding for proteins. Annual Review in Biochemistry50:349–383
    [Google Scholar]
  12. Chou P. Y., Fasman G. D.. 1978; Prediction of the secondary structure of proteins from their amino acid sequence. Advances in Enzymology Relating Areas of Molecular Biology47:145–148
    [Google Scholar]
  13. Chowdhury S. I.. 1995; Molecular basis of antigenic variation between the glycoprotein C of respiratory bovine herpesvirus1 (BHV-1) and neurovirulent (BHV-5). . : Virology213:558–568
    [Google Scholar]
  14. Collins J. K., Ayers V. K., Whetstone C. A., van Drunen Littel-van den Hurk S.. 1993; Antigenic differences between the major glycoproteins of bovine herpesvirus type 1.1 and bovine encephalitis herpesvirus type 1.3. Journal of General Virology74:1509–1517
    [Google Scholar]
  15. Davison A. J.. 1992; Channel catfish virus: a new type of herpesvirus. Virology186:9–14
    [Google Scholar]
  16. Davison A. J., Scott J. E.. 1986; The complete DNA sequence of varicella-zoster virus . Journal of. General Virology67:1759–1816
    [Google Scholar]
  17. Devereux J., Haeberli P., Smithies O.. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research12:387–395
    [Google Scholar]
  18. Dubuisson J., Thiry E., Thalasso F., Bublot M., Pastoret P.-P.. 1988; Biological and biochemical comparison of bovid herpesvirus-4 strains. Veterinary Microbiology16:339–349
    [Google Scholar]
  19. Engelhardt T., Keil G. M.. 1996; Identification and characterisation of the bovine herpesvirus 5 US4 gene and gene products. Virology225:126–135
    [Google Scholar]
  20. Engels M., Giuliani C., Wild P., Beck P., Loepfe T. M., Wyler R.. 1986; The genome of bovine herpesvirus 1 (BHV-1) strains exhibiting a neuropathogenic potential compared to known BHV-1 strains by restriction site mapping and cross- hybridisation. Virus Research6:57–73
    [Google Scholar]
  21. Fitzpatrick D. R., Zamb T. J., Parker M. D., Van Drunen Littel-van den Hurk S., Babiuk L. A., Lawman M. J. P.. 1988; Expression of bovine herpesvirus 1 glycoproteins gI and gIII in transfected murine cells. Journal of Virology62:4239–4248
    [Google Scholar]
  22. Forrester A., Farrell H., Wilkinson G., Kaye J., Davis-Poynter N., Minson T.. 1992; Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted. Journal of Virology66:341–348
    [Google Scholar]
  23. Friedli K., Metzler A. E.. 1987; Reactivity of monoclonal antibodies to proteins of a neurotropic bovine herpesvirus 1 (BHV-1) strain and to proteins of representative BHV-1 strains. Archives of Virology94:109–122
    [Google Scholar]
  24. Gompels U. A., Craxton M. A., Honess R. W.. 1988; Conservation of glycoprotein H (gH) in herpesviruses: nucleotide sequence of the gH gene from herpesvirus saimiri. Journal of General Virology69:2819–2829
    [Google Scholar]
  25. Keil G. M., Engelhardt T., Karger A., Enz M.. 1996; Bovine herpesvirus 1 Us open reading frame 4 encodes a glycoproteoglycan. Journal of Virology70:3032–3038
    [Google Scholar]
  26. Khadr A., Tikoo S. K., Babiuk L. A., van Drunen Littel-van den Hurk S.. 1996; Sequence and expression of a bovine herpesvirus-1 gene homologous to the glycoprotein K-encoding gene of herpes simplex virus-1. Gene168:189–193
    [Google Scholar]
  27. Khattar S. K., van Drunen Littel-van den Hurk S., Attah-Poku S. K., Babiuk L. A., Tikoo S. K.. 1996; Identification and characterisation of a bovine herpesvirus-1 (BHV-1) glycoprotein gL which is required for proper antigenicity, processing, and transport of BHV-1 glycoprotein gH. Virology219:66–76
    [Google Scholar]
  28. Klupp B. G., Mettenleiter T. C.. 1991; Sequence and expression of the glycoprotein gH gene of pseudorabies virus. Virology182:732–741
    [Google Scholar]
  29. Kornfeld R., Kornfeld S.. 1985; Assembly of asparagine-linked oligosaccharides. Annual Review of Biochemistry54:631–664
    [Google Scholar]
  30. Kozak M.. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eucaryotic ribosomes. Cell44:283–292
    [Google Scholar]
  31. Kreil G.. 1981; Transfer of proteins across membranes. Annual Review of Biochemistry50:317–348
    [Google Scholar]
  32. Liang X., Babiuk L. A., van Drunen Littel-van den Hurk S., Fitzpatrick D. R., Zamb T. J.. 1991; Bovine herpesvirus-1 attachment to permissive cells is mediated by its major glycoproteins gI, gIII and gIV. Journal of Virology65:1124–1132
    [Google Scholar]
  33. McGeoch D. J., Davison A. J.. 1986; DNA sequence of the herpes simplex virus type 1 gene encoding glycoprotein gH, and identification of homologues in the genomes of varicella-zoster virus and Epstein–Barr virus. Nucleic Acids Research14:4281–4292
    [Google Scholar]
  34. Maniatis T., Fritsch E. F., Sambrook J.. 1982; Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Metzler A. E., Schudel A. A., Engels M.. 1986; Bovine herpesvirus 1: molecular and antigenic characteristics of variants isolated from calves with neurological disease. Archives of Virology87:205–217
    [Google Scholar]
  36. Meyer A. L., Petrovskis E. A., Duffus W. P. H., Thomsen D. R., Post L. E.. 1991; Cloning and sequence of an infectious bovine rhinotracheitis virus (BHV-1) gene homologous to glycoprotein H of herpes simplex virus. Biochimica et Biophysica Acta1090:267–269
    [Google Scholar]
  37. Meyer G., Hanon E., Georlette D., Pastoret P.-P., Thiry E.. 1998; Bovine herpesvirus type 1 glycoprotein H is essential for penetration and propagation in cell culture. Journal of General Virology79:1983–1987
    [Google Scholar]
  38. Nicolson L., Cullinane A. A., Onions D. E.. 1990; The nucleotide sequence of an equine herpesvirus 4 gene homologue of the herpes simplex virus 1 glycoprotein H gene. Journal of General Virology71:1793–1800
    [Google Scholar]
  39. Okazaki K., Matsuzaki T., Sugahara Y., Okada J., Hasebe M., Iwamura Y., Ohnishi M., Kanno T., Shimizu M., Honda E.. 1991; BHV-1 adsorption is mediated by the interaction of glycoprotein gIII with heparin-like moiety on the cell surface. Virology181:666–670
    [Google Scholar]
  40. Okazaki K., Honda E., Kono Y.. 1994; Heparin-binding domain of bovid herpesvirus 1 glycoprotein gIII. Archives of Virology134:413–419
    [Google Scholar]
  41. Peeters B., de Wind N., Broer R., Gielkens A., Moormann R.. 1992; Glycoprotein H of pseudorabies virus is essential for entry and cell-to-cell spread of the virus. Journal of Virology66:3888–3892
    [Google Scholar]
  42. Rijsewijk F. A. M., Magdalena J., Kaashoek M., Maris-Veldhuis M. A., Gielkens A. L. J., van Oirschot J. T.. 1992; Identification and functional analysis of the glycoprotein E (gE) of bovine herpesvirus 1. In 17th International Herpesvirus Workshop, Edinburgh Scotland p245
    [Google Scholar]
  43. Rijsewijk F., Kaashoek M., Keil G., Paal H., Ruuls R., van Engelenburg F., van Oirschot J. T.. 1995; In vitro and in vivo role of the non essential glycoproteins gC, gG, gI and gE of bovine herpesvirus 1.. In Symposium on IBR and other ruminant herpesvirus infections. European Society for Virology, Liège, Belgium p.27
    [Google Scholar]
  44. Rock D. L., Hagemoser W. A., Osorio F. A., Reed D. E.. 1986; Detection of bovine herpesvirus type 1 RNA in trigeminal ganglia of latently infected rabbits by in situ hybridization. Journal of General Virology67:2515–2520
    [Google Scholar]
  45. Sanger F., Nicklen S., Coulson A. R.. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences USA:74:5463–5467
    [Google Scholar]
  46. Schwyzer M., Ackermann M.. 1996; Molecular virology of ruminant herpesviruses. Veterinary Microbiology53:17–29
    [Google Scholar]
  47. Scott S. D., Smith G. D., Ross N. L. J., Binns M. M.. 1993; Identification and sequence of the homologues of the herpes simplex virus type 1 glycoprotein H in Marek’s disease virus and the herpesvirus of turkeys. Journal of General Virology74:1185–1190
    [Google Scholar]
  48. Seal B. S., Whetstone C. A., Zamb T. J., Bello L. J., Lawrence W. C.. 1992; Relationship of bovine herpesvirus 1 immediate-early, early and late gene expression to host cellular gene transcription. Virology188:152–159
    [Google Scholar]
  49. Smith G. A., Young P. L., Mattick J. S.. 1991; Nucleotide and amino acid sequence analysis of the thymidine kinase gene of a bovine encephalitis herpesvirus. Archives of Virology119:199–210
    [Google Scholar]
  50. Tarentino A. L., Trimble R. B., Thomas H., Plummer T. H.. 1989; Enzymatic approaches for studying the structure, synthesis, and processing of glycoproteins. Methodology in Cellular Biology32:111–139
    [Google Scholar]
  51. Telford E. A. R., Watson M. S., McBride K., Davison A. J.. 1992; The DNA sequence of equine herpesvirus-1. Virology189:304–316
    [Google Scholar]
  52. Tikoo S. K., Fitzpatrick D. R., Babiuk L. A., Zamb T. J.. 1990; Molecular cloning, sequencing, and expression of functional bovine herpesvirus 1 glycoprotein IV in transfected bovine cells. Journal of Virology64:5132–5142
    [Google Scholar]
  53. van Drunen Littel-van den Hurk S., Babiuk L. A.. 1985; Effect of tunicamycin and monensin on biosynthesis, transport, and maturation of bovine herpesvirus type-1 glycoproteins. Virology143:104–118
    [Google Scholar]
  54. van Drunen Littel-van den Hurk S., Babiuk L. A.. 1986; Synthesis and processing of bovine herpesvirus-1 glycoproteins. Journal of Virology59:401–410
    [Google Scholar]
  55. van Drunen Littel-van den Hurk S., Khattar S., Tikoo S. K., Babiuk L. A., Baranowski E., Plainchamp D., Thiry E.. 1996; Glycoprotein H (gII/gp108) and glycoprotein L form a functional complex which plays a role in penetration, but not in attachment, of bovine herpesvirus 1. Journal of General Virology77:1515–1520
    [Google Scholar]
  56. von Heinje G.. 1986; A new method for predicting signal sequence cleavage sites . Nucleic Acids. Research14:4683–4690
    [Google Scholar]
  57. Whitbeck J. C., Bello L. J., Lawrence W. C.. 1988; Comparison of the bovine herpesvirus type 1 gI gene and the herpes simplex virus type 1 B gene. Journal of Virology62:3319–3327
    [Google Scholar]
  58. Wu S. X., Zhu X. P., Letchworth G. J.. 1998; Bovine herpesvirus 1 glycoprotein M forms a disulfide-linked heterodimer with the U(L)49.5 protein. Journal of Virology72:3029–3036
    [Google Scholar]
  59. Wyler R., Engels M., Schwyzer M.. 1989; Infectious bovine rhinotracheitis/vulvovaginitis (BHV-1).. In Herpesvirus Diseases of Cattle, Horses and Pigs. Developments in Veterinary Virology pp.1–72 Edited by Wittmann G.. Boston: Kluwer;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-11-2849
Loading
/content/journal/jgv/10.1099/0022-1317-80-11-2849
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error