%0 Journal Article %A Urcuqui-Inchima, Silvio %A Maia, Ivan G. %A Drugeon , Gabrièle %A Haenni, Anne-Lise %A Bernardi, Françoise %T Effect of mutations within the Cys-rich region of potyvirus helper component-proteinase on self-interaction %D 1999 %J Journal of General Virology, %V 80 %N 11 %P 2809-2812 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-80-11-2809 %I Microbiology Society, %X The first ∼60 amino acids of the N-terminal part of the potyvirus helper component-proteinase (HC-Pro) include highly conserved residues comprising a Cys-rich region. In the present study, the domain in Potato virus Y sufficient for self-interaction was mapped using the yeast two-hybrid system to the 83 N-terminal amino acids of HC-Pro. Mutations in the conserved His and two Cys residues within the Cys-rich region have a strong debilitating effect on self-interaction when introduced in the full-length HC-Pro, but not when introduced in the N-terminal fragment. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-80-11-2809