RT Journal Article SR Electronic(1) A1 MacKenzie, Jane A1 Gray, Diane A1 Pinto-Paes, Roberto A1 Barrezueta, Luis F. M. A1 Armstrong, Alison A. A1 Alexander, Freda A. A1 McGeoch, Duncan J. A1 Jarrett, Ruth F. YR 1999 T1 Analysis of Epstein–Barr virus (EBV) nuclear antigen 1 subtypes in EBV-associated lymphomas from Brazil and the United Kingdom JF Journal of General Virology, VO 80 IS 10 SP 2741 OP 2745 DO https://doi.org/10.1099/0022-1317-80-10-2741 PB Microbiology Society, SN 1465-2099, AB EBNA-1 is the only viral protein consistently expressed in all cells latently infected by Epstein–Barr virus (EBV). There is a high frequency of sequence variation within functionally important domains of EBNA-1, with five subtypes identified. Individuals may be infected with multiple EBV strains (classified according to EBNA-1 subtype), but Burkitt’s lymphoma (BL) tumours carry a single subtype and exhibit some subtype preference. Subtype variation has also been related to geographical location. In the present study EBNA-1 polymorphisms were examined in a series of haematological malignancies from two distinct geographical regions, Brazil and the United Kingdom. Nucleotide sequence analysis of the carboxy-terminal region of EBNA-1 in 34 cases revealed six distinct sequences, some of which are novel. A new subtype, named V-Ala, was identified. EBNA-1 subtype in tumours differed markedly according to geographical location. In contrast to previous studies, we found evidence of EBNA-1 sequence variation within individual BL tumour samples., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-80-10-2741