1887

Abstract

Mutant polioviruses (PV) have been previously found to be capable of establishing persistent infections in HEp-2c cells. Together, two amino acid substitutions in the viral capsid of a type 3 poliovirus (PV-3), at positions VP2 and VP1, are sufficient to confer the persistent phenotype to a normally lytic virus. When susceptible cells are infected, the double mutant T7L+2L 1N undergoes unique conformational changes in the capsid, modifying its sedimentation coefficient from 160S to 147S. In the present study, we have further investigated mutant PV decapsidation and, in particular, the effect of each determinant independently. Our results indicate that the novel 147S form was also generated by a mutant carrying only the determinant 1N. This form was not produced as a result of inherent capsid instability and it was generated only upon specific PV–host cell interactions. The second viral determinant, 2L, also modified receptor-induced conformational changes, although differently from 1N.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-80-10-2601
1999-10-01
2019-10-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/80/10/0802601a.html?itemId=/content/journal/jgv/10.1099/0022-1317-80-10-2601&mimeType=html&fmt=ahah

References

  1. Colbère-Garapin, F. , Christodoulou, C. , Crainic, R. & Pelletier, I. ( 1989; ). Persistent poliovirus infection of human neuroblastoma cells. Proceedings of the National Academy of Sciences, USA 86, 7590-7594 .[CrossRef]
    [Google Scholar]
  2. Colston, E. & Racaniello, V. R. ( 1994; ). Soluble receptor- resistant poliovirus mutants identify surface and internal capsid residues that control interaction with the cell receptor. EMBO Journal 13, 5855-5862 .
    [Google Scholar]
  3. Curry, S. , Chow, M. & Hogle, J. M. ( 1996; ). The poliovirus 135S particle is infectious. Journal of Virology 70, 7125-7131 .
    [Google Scholar]
  4. Dove, A. W. & Racaniello, V. R. ( 1997; ). Cold-adapted poliovirus mutants bypass a postentry replication block. Journal of Virology 71, 4728-4735 .
    [Google Scholar]
  5. Duncan, G. , Pelletier, I. & Colbère-Garapin, F. ( 1998; ). Two amino acid substitutions in the type 3 poliovirus capsid contribute to the establishment of persistent infection in HEp-2c cells by modifying virus–receptor interactions. Virology 241, 14-29.[CrossRef]
    [Google Scholar]
  6. Filman, D. J. , Syed, R. , Chow, M. , Macadam, A. J. , Minor, P. D. & Hogle, J. M. ( 1989; ). Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus. EMBO Journal 8, 1567-1579.
    [Google Scholar]
  7. Flore, O. , Fricks, C. E. , Filman, D. J. & Hogle, J. M. ( 1990; ). Conformational changes in poliovirus assembly and cell entry. In Seminars in Virology , pp. 429-438. Edited by J. M. Hogle. London: W. B. Saunders.
  8. Fricks, C. E. & Hogle, J. M. ( 1990; ). Cell-induced conformational change in poliovirus: externalization of the amino terminus of VP1 is responsible for liposome binding. Journal of Virology 64, 1934-1945 .
    [Google Scholar]
  9. Gomez Yafal, A. , Kaplan, G. , Racaniello, V. R. & Hogle, J. M. ( 1993; ). Characterization of poliovirus conformational alteration mediated by soluble cell receptors. Virology 197, 501-505.[CrossRef]
    [Google Scholar]
  10. Harber, J. , Bernhardt, G. , Lu, H. H. , Sgro, J. Y. & Wimmer, E. ( 1995; ). Canyon rim residues, including antigenic determinants, modulate serotype-specific binding of polioviruses to mutants of the poliovirus receptor. Virology 214, 559-570.[CrossRef]
    [Google Scholar]
  11. Kaplan, G. , Freistadt, M. S. & Racaniello, V. R. ( 1990; ). Neutralization of poliovirus by cell receptors expressed in insect cells. Journal of Virology 64, 4697-4702 .
    [Google Scholar]
  12. Koike, S. , Horie, H. , Ise, I. , Okitsu, A. , Yoshida, M. , Iizuka, N. , Takeuchi, K. , Takegami, T. & Nomoto, A. ( 1990; ). The poliovirus receptor protein is produced both as membrane-bound and secreted forms. EMBO Journal 9, 3217-3224.
    [Google Scholar]
  13. Mendelsohn, C. L. , Wimmer, E. & Racaniello, V. R. ( 1989; ). Cellular receptor for poliovirus: molecular cloning, nucleotide sequence and expression of a new member of the immunoglobulin superfamily. Cell 56, 855-865.[CrossRef]
    [Google Scholar]
  14. Minor, P. D. , Ferguson, M. , Evans, D. M. A. , Almond, J. W. & Icenogle, J. P. ( 1986; ). Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67, 1283-1291 .[CrossRef]
    [Google Scholar]
  15. Olson, N. H. , Kolatkar, P. R. , Oliveira, M. A. , Cheng, R. H. , Greve, J. M. , McClelland, A. , Baker, T. S. & Rossmann, M. G. ( 1993; ). Structure of a human rhinovirus complexed with its receptor molecule. Proceedings of the National Academy of Sciences, USA 90, 507-511.[CrossRef]
    [Google Scholar]
  16. Pelletier, I. , Couderc, T. , Borzakian, S. , Wyckoff, E. , Crainic, R. , Ehrenfeld, E. & Colbère-Garapin, F. ( 1991; ). Characterization of persistent poliovirus mutants selected in human neuroblastoma cells. Virology 180, 729-737.[CrossRef]
    [Google Scholar]
  17. Pelletier, I. , Duncan, G. & Colbère-Garapin, F. ( 1998a; ). One amino acid change on the capsid surface of poliovirus Sabin 1 allows the establishment of persistent infections in HEp-2c cell cultures. Virology 241, 1-13.[CrossRef]
    [Google Scholar]
  18. Pelletier, I. , Duncan, G. , Pavio, N. & Colbère-Garapin, F. ( 1998b; ). Molecular mechanisms of poliovirus persistence: key role of capsid determinants during the establishment phase. Cellular and Molecular Life Sciences 54, 1385-1402.[CrossRef]
    [Google Scholar]
  19. Wimmer, E. , Hellen, C. U. & Cao, X. M. ( 1993; ). Genetics of poliovirus. Annual Review of Genetics 27, 353-436.[CrossRef]
    [Google Scholar]
  20. Wimmer, E. , Harber, J. J. , Bibb, J. A. , Gromeier, M. , Lu, H.-H. & Bernhardt, G. ( 1994; ). Poliovirus receptor. In Cellular Receptors for Animal Viruses, pp. 101-127. Edited by E. Wimmer. Cold Spring Harbor Laboratory, NY: Cold Spring Harbor Laboratory Press.
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-80-10-2601
Loading
/content/journal/jgv/10.1099/0022-1317-80-10-2601
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error