1887

Abstract

Filamentous particles of beet yellows closterovirus (BYV) are built of two related capsid proteins, of which the minor species, p24, forms a 75 nm tail at one end of the virion. In the present work, we used polyclonal antibodies against p24 for isolating the ‘tailed’ virion segments from sonicated BYV particle preparations. The [gamma-32P]ATP-labelled RNA obtained from the antibody-selected particle segments consistently showed stronger hybridization with the 5′-terminal BYV cDNA clones than with the 3′-terminal cDNA clones. These data clearly indicate that it is the 5′-terminal portion of the closterovirus RNA genome that is encapsidated by p24.

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1999-01-01
2022-05-20
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