1887

Abstract

Bovine papillomavirus type 4 (BPV-4) is a mucosal epitheliotropic virus that is a causative agent in alimentary carcinoma of cattle. The long control region (LCR) of this virus controls expression of the transforming proteins, E8 and E7. Deletion mutants of the LCR were prepared and assayed for their ability to activate transcription from the LCR promoter in primary bovine palate keratinocytes (the natural target cell for BPV-4) and fibroblasts. The LCR was at least an order of magnitude more active in keratinocytes than in fibroblasts. An epithelial specific enhancer was identified that activated transcription from the SV40 promoter to levels identical to the full-length LCR. One of the active sites in the enhancer is 100% conserved in the LCR of human papillomavirus type 16. The results demonstrate that the BPV-4 LCR has an epithelial specific enhancer, which offers the opportunity to study epithelial specific transcriptional regulation of papillomavirus promoters.

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1999-01-01
2022-08-17
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References

  1. Apt D., Chong T., Liu Y., Bernard H. U. 1993; Nuclear factor I and epithelial cell-specific transcription of human papillomavirus type 16. Journal of Virology 67:4455–4463
    [Google Scholar]
  2. Apt D., Liu Y., Bernard H. U. 1994; Cloning and functional analysis of spliced isoforms of human nuclear factor I-X: interference with transcriptional activation by NF1/CTF in a cell-type specific manner. Nucleic Acids Research 22:3825–3833
    [Google Scholar]
  3. Apt D., Watts R. M., Suske G., Bernard H. U. 1996; High Sp1/Sp3 ratios in epithelial cells during epithelial differentiation and cellular transformation correlate with the activation of the HPV-16 promoter. Virology 224:281–291
    [Google Scholar]
  4. Bauknecht T., Shi Y. 1998; Overexpression of C/EBPβ represses human papillomavirus type 18 upstream regulatory region activity in HeLa cells by interfering with the binding of TATA-binding protein. Journal of Virology 72:2113–2124
    [Google Scholar]
  5. Bauknecht T., See R. H., Shi Y. 1996; A novel C/EBPβ–YY1 complex controls the cell-type-specific activity of the human papillomavirus type 18 upstream regulatory region. Journal of Virology 70:7695–7705
    [Google Scholar]
  6. Campo M. S., O′Neil B. W., Barron R. J., Jarrett W. F. H. 1994; Experimental reproduction of the papilloma-carcinoma complex of the alimentary canal in cattle. Carcinogenesis 15:1597–1601
    [Google Scholar]
  7. Chong T., Apt D., Gloss B., Isa M., Bernard H. U. 1991; The enhancer of human papillomavirus type 16: binding sites for the ubiquitous transcription factors oct-1, NFA, TEF-2, NF1, and AP-1 participate in epithelial cell-specific transcription. Journal of Virology 65:5933–5943
    [Google Scholar]
  8. Cuthill S., Sibbet G. J., Campo M. S. 1993; Characterization of a nuclear factor, papilloma enhancer binding factor-1, that binds the long control region of human papillomavirus type 16 and contributes to enhancer activity. Molecular Carcinogenesis 8:96–104
    [Google Scholar]
  9. Desaintes C., Demeret C. 1996; Control of papillomavirus DNA replication and transcription. Seminars in Cancer Biology 7:339–347
    [Google Scholar]
  10. Dong X. P., Stubenrauch F., Beyer-Finkler E., Pfister H. 1994; Prevalence of deletions of YY1-binding sites in episomal HPV 16 DNA from cervical cancers. International Journal of Cancer 58:803–808
    [Google Scholar]
  11. Gloss B., Bernard H. U., Seedorf K., Klock G. 1987; The upstream regulatory region of the human papilloma virus-16 contains an E2 protein-independent enhancer which is specific for cervical carcinoma cells and regulated by glucocorticoid hormones. EMBO Journal 6:3735–3743
    [Google Scholar]
  12. Gloss B., Chong T., Bernard H. U. 1989; Numerous nuclear proteins bind the long control region of human papillomavirus type 16: a subset of 6 of 23 DNase I-protected segments coincides with the location of the cell-type-specific enhancer. Journal of Virology 63:1142–1152
    [Google Scholar]
  13. Hoppe-Seyler F., Butz K., zur Hausen H. 1991; Repression of the human papillomavirus type 18 enhancer by the cellular transcription factor Oct-1. Journal of Virology 65:5613–5618
    [Google Scholar]
  14. Jackson M. E., Campo M. S. 1991; Positive and negative E2-independent regulatory elements in the long control region of bovine papillomavirus type 4. Journal of General Virology 72:877–883
    [Google Scholar]
  15. Jackson M. E., Campo M. S. 1995; Both viral E2 protein and the cellular factor PEBP2 regulate transcription via E2 consensus sites within the bovine papillomavirus type 4 long control region. Journal of Virology 69:6038–6046
    [Google Scholar]
  16. McCaffery R. E., Jackson M. E. 1994; An element binding a C/EBP-related transcription factor contributes to negative regulation of the bovine papillomavirus type 4 long control region. Journal of General Virology 75:3047–3056
    [Google Scholar]
  17. Morgan I. M., Grindlay G. J., Campo M. S. 1998; Epithelial specific transcriptional regulation of the bovine papillomavirus 4 promoter by E2. Journal of General Virology 79:501–508
    [Google Scholar]
  18. Morris P. J., Dent C. L., Ring C. J., Latchman D. S. 1993; The octamer binding site in the HPV-16 regulatory region produces opposite effects on gene expression in cervical and non-cervical cells. Nucleic Acids Research 21:1019–1023
    [Google Scholar]
  19. Nakshatri H., Pater M. M., Pater A. 1990; Ubiquitous and cell-type-specific protein interactions with human papillomavirus type 16 and type 18 enhancers. Virology 178:92–103
    [Google Scholar]
  20. O′Connor M., Bernard H. U. 1995; Oct-1 activates the epithelial-specific enhancer of human papillomavirus type 16 via a synergistic interaction with NF1 at a conserved composite regulatory element. Virology 207:77–88
    [Google Scholar]
  21. O′Connor M. J., Tan S. H., Tan C. H., Bernard H. U. 1996; YY1 represses human papillomavirus type 16 transcription by quenching AP-1 activity. Journal of Virology 70:6529–6539
    [Google Scholar]
  22. Offord E. A., Beard P. 1990; A member of the activator protein 1 family found in keratinocytes but not in fibroblasts required for transcription from a human papillomavirus type 18 promoter. Journal of Virology 64:4792–4798
    [Google Scholar]
  23. Sibbet G. J., Cuthill S., Campo M. S. 1995; The enhancer in the long control region of human papillomavirus type 16 is up-regulated by PEF-1 and down-regulated by Oct-1. Journal of Virology 69:4006–4011
    [Google Scholar]
  24. Thierry F., Spyrou G., Yaniv M., Howley P. 1992; Two AP1 sites binding JunB are essential for human papillomavirus type 18 transcription in keratinocytes. Journal of Virology 66:3740–3748
    [Google Scholar]
  25. Wang H., Liu K., Yuan F., Berdichevsky L., Taichman L. B., Auborn K. 1996; C/EBPβ is a negative regulator of human papillomavirus type 11 in keratinocytes. Journal of Virology 70:4839–4844
    [Google Scholar]
  26. zur Hausen H. 1991; Human papillomaviruses in the pathogenesis of anogenital cancer. Virology 184:9–13
    [Google Scholar]
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