1887

Abstract

Attempts to isolate phages active on strains of from sewage (Coetzee, de Klerk & Sacks, 1960) and by u.v. light-induction of these strains were unsuccessful (Coetzee & de Klerk, 1962). In renewed attempts to derive phages from , 80 strains were treated with mitomycin C. Early log phase cultures of the strains in MRS broth (de Man, Rogosa & Sharpe, 1960) were exposed to mitomycin C (1 µg./ml.) for 20 min. at 37°. The cells were harvested and suspended in equal volumes of fresh warm broth and incubated for 6 hr. After centrifugation, culture supernatant fluids were sterilized with chloroform and dilutions spotted on lawns of all the 80 strains and also 98 and 50 strains, as previously described (de Klerk & Coetzee, 1961). No plaques were ever seen, but 16 of the 80 supernatant fluids produced zones of growth inhibition on some of the indicators.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-8-3-231
1970-09-01
2019-11-13
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-8-3-231
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error