1887

Abstract

SOMMAIRE

L’isolement de mutants spontanés thermosensibles a permis d’entreprendre l’étude génétique du Virus de la Stomatite Vésiculaire. Ces mutants donnent des plages à 30° sur un tapis de fibroblastes de poulet mais n’en donnent pas à 39.8 ± 0.2°. Cet article rapporte le résultat de tests de complémentation conduisant à classer 71 mutants en 5 groupes.

Seventy-one spontaneous thermosensitive mutants of Vesicular Stomatitis Virus were isolated and tested for complementation. The spontaneous mutation rate seems to be very high since 2.3% of mutants were found in wild-type clones. Experiments were performed at 30° (permissive temperature) and 39.8° (non-permissive temperature). The mutants can be classified into five complementation groups. 58 mutants fall into the first group.

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1970-09-01
2021-09-24
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References

  1. Benzer S. 1961; On the topography of the genetic fine structure. Proceedings of the National Academy of Sciences of the United States of America 47:403
    [Google Scholar]
  2. Breeze D. C., Subak Sharpe H. 1967; The mutability of small plaque-forming Encephalomyocarditis Virus. Journal of General Virology 1:8l
    [Google Scholar]
  3. Burge B. W., Pfefferkorn E. R. 1966; Isolation and characterization of conditional lethal mutants of Sindbis virus. Virology 30:204
    [Google Scholar]
  4. Dulbecco R. 1952; Production of plaques in monolayer tissue cultures by single particles of animal virus. Proceedings of the National Academy of Science of the United States of America 38:747
    [Google Scholar]
  5. Flamand A. 1969; Etude des mutants thermosensibles du Virus de la Stomatite Vesiculaire. Mise au point d’un test de complémentation. Compte rendu hebdomadaire des séances de TAcademie des sciences, Paris 268:2305
    [Google Scholar]
  6. Kang C. Y., Prevec L. 1969; Proteins of VSV. I. Polyacrylamide Gel analysis of viral antigens. Journal of Virology 3:404
    [Google Scholar]
  7. Katz S. L., Milovanovic M. V., Enders J. F. 1958; Propagation of measles virus in culture of chick embryo cell. Proceedings of the Society for Experimental Biology and Medicine 97:23
    [Google Scholar]
  8. Lafay F. 1969; Etude des mutants thermosensibles du Virus de la Stomatite vesiculaire. Classification de quelques mutants d’apres des criteres de fonctionnement. Compte rendu hebdomadaire des seances de TAadémie des sciecnces, Paris 268:2385
    [Google Scholar]
  9. Pfefferkorn E. R., Burge B. W. 1967; Genetics and biochemistry of arbovirus temperature sensitive mutants. In The Molecular Biology of Viruses. Ed. by Colter S, Paranchych W. p 403 New York and London: Academic Press;
    [Google Scholar]
  10. Pringle C. R. 1970; Genetic characteristics of conditional lethal mutants of vesicular stomatitis virus induced by 5 fluorouracil, 5 azacytidine and ethyl methane sulfonate. Journal of Virology 5:559
    [Google Scholar]
  11. Tooze J., Weber K. 1967; Isolation and characterization of amber mutants of Bacteriophage R17. Journal of Molecular Biology 28:311
    [Google Scholar]
  12. Vigier PH. 1965; Contribution a l’etude de l’instabilite genetique du virus α de la Drosophile. Annales de genetique 9:1
    [Google Scholar]
  13. Wagner R. R., Schnaitman T. A., Snyder R. M. 1969; Structural proteins of VSV. Journal of Virology 3:395
    [Google Scholar]
  14. Wentworth B. B., Maccahon D., Cooper P. D. 1968; Production of infectious RNA and serum blocking antigen by poliovirus temperature sensitive mutants. Journal of General Virology 2:297
    [Google Scholar]
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