1887

Abstract

SUMMARY

The interaction of avian infectious bronchitis virus with the chicken embryo kidney cell was studied before and after neutralization by homotypic antiserum containing only 7s (IgG) antibody. Entry of virus into the cell, measured by the ability of antibody to neutralize only extracellular virus, occurred at 37° and 25° but not at 4°. To study entry of neutralized virus, infectious bronchitis virus was adsorbed and then neutralized at 4°. The cells were incubated at 4° (control), 25°, or 37°. Antibody was dissociated from extracellular neutralized infectious bronchitis virus with a pH 2.0 buffer solution which subsequently reactivated the virus. This acid treatment for 10 sec. did not dissociate the virus from the cell or injure the cell. Antibody did not enhance or suppress elution of infectious bronchitis virus from the cell. Neutralized infectious bronchitis virus either merged with the cytoplasmic membrane or was pinocytosed at 37° and 25°; however, the neutralized virus remained extracellular at 44°. After the neutralized virus had interacted with the cytoplasmic membrane, it was not readily accessible to acid and could not be reactivated. The entry of virus without antibody was faster than that for virus with antibody. After 1 hr at 37°, the % uncoated virus RNA in cells with unneutralized virus remained essentially constant. However, in cells with neutralized virus the % uncoated virus RNA decreased and the acid-soluble material increased simultaneously.

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/content/journal/jgv/10.1099/0022-1317-8-3-173
1970-09-01
2024-04-25
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References

  1. Bray G. A. 1960; A simple, efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical Biochemistry 1:279
    [Google Scholar]
  2. Berry D. M., Almeida J. D. 1968; The morphological and biological effects of various antisera on avian infectious bronchitis virus. Journal of General Virology 3:97
    [Google Scholar]
  3. Cunningham C. H. 1966 A Laboratory Guide in Virology 6th ed. Minneapolis: Burgess Publishing Co.;
    [Google Scholar]
  4. Cunningham C. H., Spring M. P. 1965; Somestudies of infectious bronchitis virus in cell culture. Avian Diseases 9:182
    [Google Scholar]
  5. Dales S. 1969; Viropexis of herpes simplex virus by HeLa cells. Virology 37:475
    [Google Scholar]
  6. Dales S., Kajioka R. 1964; The cycle of multiplication of vaccinia virus in Earle’s strain L cells. I. Uptake and penetration. Virology 24:278
    [Google Scholar]
  7. Hahon N., Cooke K. O. 1967; Primary virus-cell interactions in the immunofluorescence assay of Venezuelan equine encephalomyelitis virus. Journal of Virology 1:317
    [Google Scholar]
  8. Homma M., Graham A. F. 1965; Intracellular fate of Mengo virus ribonucleic acid. Journal of Bacteriology 89:64
    [Google Scholar]
  9. Hopkins S. R. 1967; Thermal stability of infectious bronchitis virus in the presence of salt solutions. Avian Diseases 10:261
    [Google Scholar]
  10. Hoyle L., Horne R. W., Waterson A. P. 1962; The structure and composition of the Myxoviruses. III. The interaction of influenza virus particles with cytoplasmic particles derived from normal chorioallantoic membrane cells. Virology 17:533
    [Google Scholar]
  11. Joklik W. K. 1964; The intracellular fate of rabbitpox virus rendered noninfectious by various reagents. Virology 22:620
    [Google Scholar]
  12. Keller R. 1968; Studies on the mechanism of the enzymatic reactivation of antibody-neutralized poliovirus. Journal of Immunology 100:1071
    [Google Scholar]
  13. Mandel B. 1962; Early stages of virus-cell interaction as studied by using antibody. Cold Spring Harbor Symposia on Quantitative Biology 27:123
    [Google Scholar]
  14. Mandel B. 1967a; The interaction of neutralized poliovirus with HeLa cells. II. Elution, penetration, uncoating. Virology 31:248
    [Google Scholar]
  15. Mandel B. 1967b; The relationship between penetration and uncoating of poliovirus in HeLa cells. Virology 31:702
    [Google Scholar]
  16. Martin R. G., Ames B. N. 1961; A method for determining the sedimentation behaviour of enzymes: Application to protein mixtures. Journal of Biological Chemistry 236:1372
    [Google Scholar]
  17. Morgan C., Howe C. 1968; Structure and development of viruses as observed in the electron microscope. IX. Entry of parainfluenza I (Sendai) virus. Journal of Virology 2:1122
    [Google Scholar]
  18. Morgan C., Rose H. M. 1968; Structure and development of viruses as observed in the electron microscope. VIII. Entry of influenza virus. Journal of Virology 2:925
    [Google Scholar]
  19. Morgan C., Rose H. M., Mednis B. 1968; Electron microscopy of herpes simplex virus. I. Entry. Journal of Virology 2:507
    [Google Scholar]
  20. Rubin H., Franklin R. M. 1957; On the mechanism of Newcastle disease virus neutralization by immune serum. Virology 3:84
    [Google Scholar]
  21. Silverstein S. C., Marcus P. I. 1964; Early stages of Newcastle disease virus-HeLa interaction: An electron microscope study. Virology 23:370
    [Google Scholar]
  22. Stinski M. F., Cunningham C. H. 1969; Neutralizing antibody complex of infectious bronchitis virus. Journal of Immunology 102:720
    [Google Scholar]
  23. Wallis C., Melnick J. L. 1967; Virus aggregation as the cause of the nonneutralizable persistent fraction. Journal of Virology 1478
    [Google Scholar]
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