Fate of Adenovirus Types 2 and 12 in Infected Serial Cultures of Non-primate Origin Free

Abstract

SUMMARY

In RHF-1 cells infected with either adenovirus 2 or 12, the formation of infectious virus and antigens decreased with each successive passage of cells until the virus was ultimately eliminated from the cultures. These cultures then emerged into a new phase in which some virus-induced proteins were present in at least a small proportion of cells. Adenovirus 2 fibre antigen persisted throughout the 15th subculture, whereas adenovirus 12 early (T) and late (fibre) antigens were carried throughout the 30th subculture over a period of 600 days. Virus-free but antigen-containing cells may therefore have possessed at least a portion of the virus genome. Shortly after the disappearance of virus, distinct multilayered foci of cells emerged in both lines. This phenomenon became a characteristic feature of the cultures only in the adenovirus 12 line.

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1970-08-01
2024-03-29
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References

  1. Ankudas M. M., Khoobyarian N. 1962; Rabbit heart cell cultures, strain RHF-1. II. Behavior of adenovirus types 1 to 4. Journal of Bacteriology 84:1287
    [Google Scholar]
  2. Burns W. H., Black P. H. 1969; Induction experiments with adenovirus and polyoma virus transformed cell lines. International Journal of Cancer 4:204
    [Google Scholar]
  3. Fujinaga K., Green M. 1966; The mechanism of viral carcinogenesis by DNA mammalian viruses: Viral-specific RNA in polyribosomes of adenovirus tumors and transformed cells. Proceedings of the National Academy of Sciences of the United States of America 55:1567
    [Google Scholar]
  4. Ginsberg H. S. 1958 In Symposium on Latency and Masking in Viral and Rickettsial Infections p. 157 Ed. by. Walker D. L., Hanson R. P, Evans A. S. Minneapolis, Minnesota: Burgess Publishing Company;
    [Google Scholar]
  5. Hilleman M. R., Werner J. H. 1954; Recovery of new agent from patients with acute respiratory illness. Proceedings of the Society for Experimental Biology and Medicine 85:183
    [Google Scholar]
  6. Hollinshead A. C., Huebner R. S. 1966; Isolation and analysis of adenovirus 12 sub-units from adenovirus type 12-infected cells. Nature, London 211:890
    [Google Scholar]
  7. Khoobyarian N., Palmer C. G. 1961; Rabbit heart cell culture, strain RHF-1. I. Growth, cytology, histology and susceptibility to some inclusion-inducing viruses. Journal of the National Cancer Institute 26:755
    [Google Scholar]
  8. Klemperer H. G., Pereira H. G. 1959; Study of adenovirus antigens fractionated by chromatography on DEAE-celluIose. Virology 9:536
    [Google Scholar]
  9. Kusano T., Yamane I. 1967; Transformation in vitro of the embryonal hamster brain cells by human adenovirus type 12. Tohoku Journal of Experimental Medicine 93:141
    [Google Scholar]
  10. Landau B. J., Larson V. M., Devers G. A., Hilleman M. R. 1966; Studies on induction and virus from adenovirus and SV40 hamster tumors. 1. Chemical and physical agents. Proceedings of the Society for Experimental Biology and Medicine 122:1174
    [Google Scholar]
  11. Larson V. M., Gosnell P. A., Hilleman M. R. 1966; Studies on induction from adenovirus and SV40 hamster tumors. 2. ‘Helper’ viruses. Proceedings of the Society for Experimental Biology and Medicine 122:1182
    [Google Scholar]
  12. Pereira H. G., De Figueiredo M. V. T. 1962; Mechanism of hemagglutination by adenovirus types 1, 2, 4, 5 and 6. Virology 18:1
    [Google Scholar]
  13. Puck T. T., Cieciura S. J., Robinson A. 1958; Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. Journal of Experimental Medicine 108:945
    [Google Scholar]
  14. Reed L. J., Muench H. A. 1938; A simple method of estimating fifty per cent end-points. American Journal of Hygiene 27:493
    [Google Scholar]
  15. Schlesinger R. W. 1959; Adenoviruses: the nature of the virions and of controlling factors in productive or abortive infection and tumorigenesis. Advances in Virus Research 14:1
    [Google Scholar]
  16. Schmidt N. J., King C. J., Lennette E. H. 1965; Hemagglutination and hemagglutination–inhibition with adenovirus type 12. Proceedings of the Society for Experimental Biology and Medicine 118:208
    [Google Scholar]
  17. Sultanian I. V., Freeman G. 1966; Enhanced growth of human embryonic cells infected with adenovirus 12. Science, New York 154:665
    [Google Scholar]
  18. Van Hoosier G. L. Jun., Gist C., Taylor G., Trentin J. J. 1964; Persistent infection of human cells (HeLa) with adenovirus type 12. Proceedings of the Society for Experimental Biology and Medicine 115:591
    [Google Scholar]
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