In KB cells doubly infected with vaccinia virus and Frog virus 3 (FV 3) at 37°, a non-permissive temperature for FV 3, the replication of vaccinia virus DNA was inhibited. The inhibition, which was as effective with gamma-irradiated or heat-inactivated virus as with live FV 3, was dependent upon the multiplicity of infection with FV 3. A 50% inhibition of vaccinia DNA synthesis was observed when the cells were pretreated with 0.2 p.f.u./cell of purified FV 3. Inhibition was not related to a toxic effect of FV 3 on the cells, as there was no auto-inhibition of the replicating FV 3 DNA in KB cells incubated 3 hr at 37° before incubation at 26°.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error