1887

Abstract

SUMMARY

Chick embryo lethal orphan virus induced both T and virus capsid antigens during cytolytic infection of chick kidney cells. The T antigen was produced relatively early, 8 or 9 hr after infection, while virus capsid antigen was first detected after 15 to 16 hr. The T antigen appeared as a fine granular fluorescence and also as small blobs confined to the cell nucleus. T antigen was detected in virus-infected hamster, mouse and human cells, but was present in very few cells compared with chick kidney monolayers, where almost all cells contained antigen. The virus capsid antigen had a more complex morphology, with fluorescing spots and blobs in the nucleus; it was also detected as a diffuse fluorescence in the cytoplasm later in infection. The production of virus capsid antigen in chick kidney cells was partially inhibited by cytosine arabinoside (100 µg./ml.) or 5-fluoro-2′-deoxyuridine (30 µg./ml.). These inhibitors of DNA viruses had no effect on the production of virus-induced T antigen in chick kidney cells.

No antigenic relationship was detected by immunofluorescence or complement-fixation tests between chick embryo lethal orphan virus and T antigens of adenovirus type 12. In addition, the adenovirus group hexon antigen was not detected in preparations of chick embryo lethal orphan virus by complement-fixation or immunofluorescence tests.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-8-1-33
1970-07-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/8/1/JV0080010033.html?itemId=/content/journal/jgv/10.1099/0022-1317-8-1-33&mimeType=html&fmt=ahah

References

  1. Anderson J., Yates V. J., Jasty V., Mancini L. O. 1969; In vitro transformation by an avian adenovirus (CELO). 1. Hamster-embryo fibroblast cultures. Journal of the National Cancer Institute 42:1
    [Google Scholar]
  2. Andrewes C. H., Pereira H. G. 1967 Viruses of Vertebrates London: Bailliere, Tindall and Cassell;
    [Google Scholar]
  3. Black P. H., Rowe W. P., Turner H. S., Huebner R. J. 1963; A specific complement-fixing antigen present in SV 40 tumour and transformed cells. Proceedings of the National Academy of Sciences, United States of America 50:1148
    [Google Scholar]
  4. Blatt W. F., Robinson S. M., Robbins F. M., Saravis C. A. 1967; An ultrafiltration membrane for the resolution and purification of bovine alpha-lactalbumin. Analytical Biochemistry 18:81
    [Google Scholar]
  5. Epstein M. A. 1958; An investigation into the purifying effect of a fluorocarbon on vaccinia virus. British Journal of Experimental Pathology 39:436
    [Google Scholar]
  6. Habel K. 1965; Specific complement fixing antigens in polyoma tumours and transformed cells. Virology 25:55
    [Google Scholar]
  7. Hayashi K., Russell W. C. 1968; A study of the development of adenovirus antigens by the immuno-fluorescent technique. Virology 34:470
    [Google Scholar]
  8. Hoggan M. D., Rowe W. P., Black P. H., Huebner R. J. 1965; Production of ‘tumour specific’ antigens by oncogenic viruses during acute cytolytic infections. Proceedings of the National Academy of Sciences, United States of America 53:12
    [Google Scholar]
  9. Huebner R. J. 1967; Adenovirus-directed tumourand T antigens. Perspectives in Virology 5:147
    [Google Scholar]
  10. Kawamura H., Sato T., Tsubahara H., Isogai S. 1963; Isolation of Celo virus from chicken trachea. National Institute of Animal Health Quarterly 3:1
    [Google Scholar]
  11. Kawamura H., Tsubahara H. 1963; Serological relationship between CELO and GAL viruses. National Institute Animal Health Quarterly 3:77
    [Google Scholar]
  12. Oxford J. S., Schild G. C. 1968; Immunofluorescent studies on the inhibition of influenza A and B viruses in mammalian cell cultures by amines and ammonium compounds. Journal of General Virology 2:377
    [Google Scholar]
  13. Oxford J. S., Potter C. W. 1969; Chick embryo lethal orphan (CELO) virus as a possible contaminant of egg grown virus vaccines. Journal of Hygiene, Cambridge 67:41
    [Google Scholar]
  14. Petek M., Felloga B., Zolletto R. 1963; Biological properties of CELO virus; stability to various agents and electron microscopy. Avian Diseases 1:38
    [Google Scholar]
  15. Pope J. H., Rowe W. P. 1964a; Detection of specific antigen in SV 40-transformed cells by immune-fluorescence. Journal of Experimental Medicine 120:121
    [Google Scholar]
  16. Pope J. H., Rowe W. P. 1964b; Immunofluorescent studies of adenovirus 12 tumours and of cells transformed or infected by adenoviruses. Journal of Experimental Medicine 120:577
    [Google Scholar]
  17. Potter C. W., Oxford J. S. 1969; Specific tumour antigen induced by chick embryo lethal orphan (CELO) virus. Journal of General Virology 4:287
    [Google Scholar]
  18. Rapp F., Butel J. S., Feldman L. A., Kitahara T., Melnick J. L. 1965; Differential effects of inhibitors on the steps leading to the formation of SV 40 tumour and virus antigens. Journal of Experimental Medicine 121:935
    [Google Scholar]
  19. Sarma P. S., Huebner R. J., Lane W. T. 1965; Induction of tumours in hamsters with an avian adenovirus (CELO). Science, New York 148:1108
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-8-1-33
Loading
/content/journal/jgv/10.1099/0022-1317-8-1-33
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error