1887

Abstract

SUMMARY

Mithramycin inhibited RNA synthesis in chick embryo cells in culture almost as efficiently as actinomycin D, although inhibition was considerably delayed. There was no direct effect on cellular protein synthesis. Mithramycin interfered with the multiplication of fowl plague virus (influenza A) but had little effect on the multiplication of Newcastle disease virus (parainfluenza). Ten µg./ml. of mithramycin, when added immediately after infection, preferentially inhibited the synthesis of fowl plague minus strand RNA in culture, but it had only a slight effect on the production of plus strand RNA. The synthesis of virus RNA-dependent RNA polymerase and RNP-antigen was only slightly inhibited, while the production of haemagglutinin and neuraminidase was strongly affected.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-8-1-11
1970-07-01
2022-09-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/8/1/JV0080010011.html?itemId=/content/journal/jgv/10.1099/0022-1317-8-1-11&mimeType=html&fmt=ahah

References

  1. Barry R. D. 1964; The effect of actinomycin D and ultraviolet irradiation on the production of fowl plague virus. Virology 34:563
    [Google Scholar]
  2. Barry R. D., Ives D. R., Cruickshank J. G. 1962; Participation of deoxyribonucleic acid in the multiplication of influenza virus. Nature, London 194:1139
    [Google Scholar]
  3. Becht H. 1968; Properties of erythrocytes stabilized with sulfosalicylic acid and their use in an indirect hemagglutination test with influenza virus RNP-antigen. Journal of Immunology 101:18
    [Google Scholar]
  4. Breitenfeld P. M., Schafer W. 1957; The formation of fowl plague virus antigens in infected cells, as studied with fluorescent antibodies. Virology 4:328
    [Google Scholar]
  5. Cebra J. J., Goldstein G. 1955; Chromatographic purification of tetramethylrhodanine-immune globulin conjugates and their use in the cellular localization of rabbit γ–globulin polypeptide chains. Journal of Immunology 95:230
    [Google Scholar]
  6. Davenport F. M., Rott R., Schafer W. 1960; Physical and biological properties of influenza virus components obtained after ether treatment. Journal of Experimental Medicine 112:765
    [Google Scholar]
  7. Drzeniek R. 1967; Differences in splitting capacity of virus and V. cholerae neuraminidase on sialic acid substrates. Biochemical and Biophysical Research Communications 26:631
    [Google Scholar]
  8. Dulbecco R., Vogt M. 1954; One-step growth curve of western equine encephalomyelitis virus on chicken embryo cells grown in vitro and analysis of virus yields from single cells. Journal of Experimental Medicine 99:183
    [Google Scholar]
  9. Kersten W., Kersten H., Steiner F. E., Emmerich B. 1967; The effect of chromomycin and mithramycin on the synthesis of deoxyribonucleic acid and ribonucleic acids. Hoppe-SeyleVs Zeitschrift fur Physio-logische Chemie 348:1415
    [Google Scholar]
  10. Kersten W., Kersten H., Szybalski W. 1966; Physico-chemical properties of complexes between deoxyribonucleic acid and antibiotics which affect ribonucleic acid synthesis (actinomycin, daunomycin, cinerubin, nogalamycin, chromomycin, mithramycin, and olivomycin). Biochemistry 5:236
    [Google Scholar]
  11. Rott R., Scholtissek C. 1963; Investigations about the formation of incomplete forms of fowl plague virus. Journal of General Microbiology 33:303
    [Google Scholar]
  12. Rott R., Scholtissek C. 1964; Einflufi von Actinomycin auf die Vermehrung von Myxoviren. Zeitschrift für Natmrforschung 19b:316
    [Google Scholar]
  13. Rott R., Scholtissek C. 1968; Biochemical studies on influenza virus multiplication at reduced temperatures. Journal of General Virology 3:239
    [Google Scholar]
  14. Schäfer W., Zimmermann T., Schuster H. 1959; Inaktivierung verschiedener menschenund tierpathogener Virusarten sowie des Tabak-Mosaik-Virus durch salpetrige Saure. Zeitschrift fur Naturforschung 14b:632
    [Google Scholar]
  15. Scholtissek C. 1967; Nucleotide metabolism in tissue culture cells at low temperatures. II. Feedback mechanisms during the synthesis of nucleotide and deoxynucleotide triphosphates at low temperatures. Biochimica et Biophysica Acta 145:238
    [Google Scholar]
  16. Scholtissek C. 1969; Synthesis in vitro of RNA complementary to parental viral RNA by RNA polymerase induced by influenza virus. Biochimica et Biophysica Acta 179:389
    [Google Scholar]
  17. Scholtissek C., Rott R. 1964; Behavior of virus-specific activities in tissue cultures infected with myxoviruses after chemical changes of the viral ribonucleic acid. Virology 23:169
    [Google Scholar]
  18. Scholtissek C., Rott R. 1969a; Ribonucleic acid nucleotidyl transferase induced in chick fibroblasts after infection with an influenza virus. Journal of General Virology 4:125
    [Google Scholar]
  19. Scholtissek C., Rott R. 1969b; Hybridization studies with influenza virus RNA. Virology 39:400
    [Google Scholar]
  20. Scholtissek C., Rott R. 1970; Synthesis in vivo of influenza virus plus and minus strand RNA and its preferential inhibition by antibiotics. Virology (in the Press)
    [Google Scholar]
  21. Scholtissek C., Becht H., Drzeniek R. 1967; Biochemical studies on the cytopathic effect of influenza vims. Journal of General Virology 1:219
    [Google Scholar]
  22. Skehel J. J., Hay A. J., Burke D. C., Cartwright L. N. 1967; Effects of actinomycin D and 2-mercapto-I (β-4-pyridethyl) benzimidazole on the incorporation of [3H]uridine by chick embryo cells. Biochimica et Biophysica Acta 142:430
    [Google Scholar]
  23. Smith R., Henson D. 1965; Inhibition of DNA virus replication with mithramycin. Federation Proceedings. Federation of American Societies for Experimental Biology 24:159
    [Google Scholar]
  24. White D. O., Day H. M., Batchelder E. J., Cheyne L. M., Wansbrough A. J. 1965; Delay in the multiplication of influenza virus. Virology 25:289
    [Google Scholar]
  25. Yarbro J. W., Kennedy B. J., Barnum C. P. 1966; Mithramycin inhibition of ribonucleic acid synthesis. Cancer Research 26:36
    [Google Scholar]
  26. Zimmermann T., Schafer W. 1960; Effect of p-fluorophenylalanine on fowl plague virus multiplication. Virology 11:676
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-8-1-11
Loading
/content/journal/jgv/10.1099/0022-1317-8-1-11
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error