The group-specific antigen of two murine C-type RNA tumour virus types was purified to homogeneity by isoelectric-focusing of virus disrupted with tween-ether. The antigen has a molecular weight of 25,000 to 26,000 (estimated from sedimentation in sucrose relative to standards) and an isoelectric point of 6.7. The latter value was obtained from preparations of Friend, Moloney, Rauscher and Gross subgroups. The antigen was immunogenic in guinea-pigs inducing the synthesis of monospecific group-reactive antibodies. The antigen was relatively free of nucleic acid and appeared to be the only virus protein, based on labelling studies, with an isoelectric point near neutrality. Envelope antigen was localized at pH 4.5 by the electrofocusing technique.


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