The complete nucleotide sequence of the cherry green ring mottle virus (CGRMV) genome was determined to be 8372 nt excluding a 3′ poly(A) tail. Based on computer analysis and sequence comparison, five open reading frames (ORFs) were identified on the virion strand encoding: a putative RNA-dependent RNA polymerase, a triple gene block and a coat protein. Two other ORFs with Mr values over 10000 and internal to the helicase and coat protein genes, but of unknown function, were also identified. Sequence and genome structure comparisons with other filamentous viruses indicated that CGRMV is most similar to apple stem pitting virus, some carlaviruses and potexviruses. However, it is different from members of any of these virus groups in regard to sequence homology and genome organization. A chimeric fusion coat protein was expressed in E. coli and antibodies specific for the CGRMV coat protein were raised in rabbits. The antibody was used in Western blot analyses to detect the CGRMV coat protein in infected cherry tissue.
AllisonR. F., JohnsonR. E., DoughertyW. G.1986; The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154:9–20
BeckD. L., ForsterR. L., BevanM. W., BoxenK. A., LoweS. C.1990; Infectious transcripts and nucleotide sequence of cloned cDNA of the potexvirus white clover mosaic virus. Virology 177:152–158
CavileerT. D., HalpernB. T., LawrenceD. M., PodleckisE. V., MartinR. R., HillmanB. I.1994; Nucleotide sequence of the carlavirus associated with blueberry scorch and similar disease. Journal of General Virology 75:711–720
FranckiR. I. B., FauquetC. M., KnudsonD. L., BrownF.1991; Classification and Nomenclature of Viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology supplementum 2
GorbalenyaA. E., KooninE. V., DonchenkoA. P., BlinovV. M.1988; A novel superfamily of nucleoside triphosphate-binding motif containing proteins which are probably involved in duplex unwinding in DNA and RNA replication and recombination. FEBS Letters 235:16–24
GustafsonG., ArmourS. L.1986; The complete nucleotide sequence of RNA B from the type strain of barley stripe mosaic virus. Nucleic Acids Research 14:3895–3909
HillmanB. I., HearneP., RochonD., MorrisT. J.1989; Organization of tomato bushy stunt virus genome: characterization of the coat protein gene and the 3ʹ terminus. Virology 169:42–50
HuismanM. J., LinthorstH. J. M., BolJ. F., CornelissenB. J. C.1988; The complete nucleotide sequence of potato virus χ and its homologies at the amino acid level with various plus-stranded RNA viruses. Journal of General Virology 69:1789–1798
IkedaR., WatanableE., WatanableY., OkadaY.1993; Nucleotide sequence of tobamovirus Ob which can spread systemically in N gene tobacco. Journal of General Virology 74:1939–1944
JelkmannW.1994; Nucleotide sequence of apple stem pitting virus and of the coat protein gene of a similar virus from pear associated with vein yellows disease and their relationship with potex- and carlaviruses. Journal of General Virology 75:1535–1542
MacKenzieD. J., TremaineJ. H., Stace-SmithR.1989; Organization and interviral homologies of the 3ʹ-terminal portion of potato virus S RNA. Journal of General Virology 70:1053–1063
MemelinkJ., van der VlugtC. I. M., LinthorstH. J. M., DerksA.F.L.M., AsjesC. J., BolJ. F.1990; Homologies between the genomes of a carlavirus (lily symptomless virus) and a potexvirus (lily virus X) from lily plants. Journal of General Virology 71:917–924
MorozovS. Yu., MiroshnichenkoN. A., ZeleninaD. A., FedorkinO. N., SolovyevA. G., LukashevaL. I., AtaberkovJ. G.1990; Expression of RNA transcripts of potato virus χ full-length and subgenomic cDNAs. Biochimie 72:677–684
ParkerK. G., FridlandP. R., GilmerG. M.1976; Green ring mottle. In Virus Diseases and Noninfectious Diseases of Stone Fruit in North America pp 193–199 US Department of Agriculture, Agriculture Handbook;437
PruferD., TrackE., SchmitzJ., KullB., KaufmannA., RohdeW.1992; Ribosomal frameshifting in plants-a novel signal directs the - 1 frameshift in the synthesis of the putative viral replicase of potato leafroll luteovirus. EMBO Journal 11:1111–1117
RandlesJ. W., RohdeW.1990; Nicotiana velutina mosaic virus : evidence for a bipartite genome comprising 3 kb and 8 kb RNAs. Journal of General Virology 70:1861–1869
RupasovV. V., MorozovS.-Yu., KanyukaK. V., ZavrievS. K.1989; Partial nucleotide sequence of potato virus M RNA shows similarities to potex viruses in gene arrangement and the encoded amino acid sequences. Journal of General Virology 70:1861–1869
SangerF., NicklenS., CoulsonA. R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
SatoK., YoshikawaN., TakahashiT.1993; Complete nucleotide sequence of the genome of an apple isolate of apple chlorotic leaf spot virus. Journal of General Virology 74:1927–1931
SkryabinK. G., MorozovS. Yu., KraevA. S., RozanovM. N., ChernovB. K., LukashevaL. I., AtabekovJ. G.1988; Conserved and variable elements in RNA genomes of potexviruses. FEBS Letters 240:33–40
TakamatsuS., LinB., FurutaH., MakaraK.1994; RT-PCR mediated cloning and sequence analysis of lily symptomless virus coat protein gene. Annals of the Phytopathological Society of Japan 60:487–490
ZagulaK. R., ArefN. M., RamsdellD. C.1989; Purification, serology, and some properties of a mechanically transmissible virus associated with green ring mottle disease in peach and cherry. Phytopathology 79:451–456
ZhangY.-P.1996Etiological study of cherry stem pitting disease and molecular characterization of sour cherry green ring mottle virus PhD dissertation University of California, Davis, CA, USA: