1887

Abstract

Porcine circovirus (PCV) was initially recognized as a contaminant of continuous pig kidney cell lines and was not thought to be pathogenic. Antibodies reactive to the cell culture isolate of PCV (PCV PK-15) are prevalent in the swine population worldwide. Recently, PCV PK-15-like antigen and nucleic acid were demonstrated in lesions associated with wasting syndromes in pigs in North America and Europe. Monoclonal antibodies raised to circo-viruses isolated from pigs with wasting syndromes highlighted differences between these circoviruses and the PCV PK-15 cell culture isolate. This has led to speculation that a new pathogenic PCV may have emerged in the swine populations of several countries. We report the cloning and characterization of novel circovirus DNAs purified from virus isolates made from tissues of North American and European pigs with wasting syndromes. These North American and European circoviruses form a closely related group at the nucleotide sequence level (> 96% intra-group nucleotide sequence identity) but exhibit < 80% nucleotide sequence identity with the PCV PK-15 cell culture isolate. This report provides evidence for a new type of possibly pathogenic PCV. We propose that these new circoviruses should be referred to as PCV2 as opposed to the original PK-15 cell culture isolate, which should be referred to as PCV1.

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1998-09-01
2022-05-28
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References

  1. Allan G. M., Mackie D. P., McNair J., Adair B. M., McNulty M. S. 1994; Production, preliminary characterization and applications of monoclonal-antibodies to porcine circovirus. Veterinary Immunology and Immunopathology 43:357–371
    [Google Scholar]
  2. Allan G. M., McNeilly F., Cassidy J. P., Reilly G. A. C., Adair B. M., Ellis W. A., McNulty M. S. 1995; Pathog enesis of porcine circovirus - experimental infections of colostrum deprived piglets and examination of pig foetal material. Veterinary Microbiology 44:49–64
    [Google Scholar]
  3. Allan G. M, McNeilly F., Kennedy S., Daft B., Clark E. D., Ellis J. A., Haines D. M., Meehan B. M., Adair B. M. 1998; Isolation of porcine circovirus-like viruses from pigs with a wasting disease in the United States of America and Europe. Journal of Veterinary Diagnostic Investigation 10:3–10
    [Google Scholar]
  4. Altschul S. F., Gish W., Miller W., Myersand E. W., Lipman D. J. 1990; Basic logical alignment search tool. Journal of Molecular Biology 215:403–410
    [Google Scholar]
  5. Boevink P., Chu P. W. G., Keese P. 1995; Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses. Virology 207:354–361
    [Google Scholar]
  6. Buhk H. -J., Tischer I., Koch M. A. 1985; Cloning and sequencing of the porcine circovirus PCV genome. Zentralblatt fuer Bakteriologie Mikrobiologie und Hygiene series A Meeting Abstract 260:465
    [Google Scholar]
  7. Clark E. G. 1997; Post-weaning multisystemic wasting syndrome. Proceedings of the American Association of Swine Practition 28th Annual Meeting Quebec City, Canada: pp 499–501
    [Google Scholar]
  8. Daft B., Nordhausen R. W., Latimer K. S., Niagro F. D. 1996; Interstitial pneumonia and lymphadenopathy associated with circoviral infection in a six week-old pig. 39th Meeting of the American Association of Veterinary Laboratory Diagnosticians Little Rock, AR, USA: p 32
    [Google Scholar]
  9. Dulac G. C., Afshar A. 1989; Porcine circovirus antigens in PK-15 cell line(ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Canadian Journal of Veterinary Research 53:431–433
    [Google Scholar]
  10. Edwards S., Sands J. J. 1994; Evidence of circovirus infection in British pigs. Veterinary Record 134:680–681
    [Google Scholar]
  11. Ellis J., Hassard L., Clark E., Harding J., Allan G., Willson P., Strokappe J., Martin K., McNeilly F., Meehan B., Todd D., Haines D. 1998; Isolation of circovirus from lesions of pigs with postweaning multisystemic wasting syndrome. Canadian Veterinary Journal 39:44–51
    [Google Scholar]
  12. Gilbert W., Dressler D. 1968; The rolling circle model. Cold Spring Harbor Symposia on Quantitative Biology 33:473–484
    [Google Scholar]
  13. Harding J. C. 1997; Post-weaning multisystemic wasting syndrome (PMWS): preliminary epidemiology and clinical presentation. Proceedings of the American Association of Swine Practition 28th Annual Meeting Quebec City, Canada: p 503
    [Google Scholar]
  14. Harding R. M., Burns T. M., Hafner G., Dietzgen R. G., Dale J. L. 1993; Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene. Journal of General Virology 74:323–328
    [Google Scholar]
  15. Hirt B. 1967; Selective extraction of polyoma virus DNA frominfected cell cultures. Journal of Molecular Biology 36:365–369
    [Google Scholar]
  16. Ilyina T. V., Koonin E. V. 1992; Conserved sequence motifs in the initiator proteins for rolling circle DNA replication encoded by diverse replicons from eubacteria, eukaryotes and archaebacteria. Nucleic Acids Research 20:3279–3285
    [Google Scholar]
  17. Lazarowitz S. G. 1987; The molecular characterization of gemini- viruses. Plant Molecular Biology Reporter 4:177–192
    [Google Scholar]
  18. LeCann P., Albina E., Madec F., Cariolet R., Jestin A. 1997; Piglet wasting disease. Veterinary Record 141:660
    [Google Scholar]
  19. Lukert P., deBoer G. F., Dale J. L., Keese P., McNulty M. S., Randles J. W., Tischer I. 1995; The Circoviridae In Virus Taxonomy . Sixth Report of the International Committee on Taxonomy of Viruses pp 166–168 Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Edited by Vienna & New York: Springer-Verlag;
    [Google Scholar]
  20. Mankertz J., Buhk H. -J. 1990; Transcriptional analysis of porcine circovirusPCV. VIIIth International Congress of Virology Abstracts p 380 abstract P54-008
    [Google Scholar]
  21. Mankertz A., Blaess G., Mankertz J., Walter A., Buhk H. -J. 1993; Analysis of transcription and replication of porcine circovirus PCV. IXth International Congress of Virology Abstracts p 76 abstract W45-8
    [Google Scholar]
  22. Mankertz A., Persson F., Mankertz J., Blaess G., Buhk H. -J. 1997; Mapping and characterization of the origin ofDNA replication of porcine circovirus. Journal of Virology 71:2562–2566
    [Google Scholar]
  23. Mankertz A., Mankertz J., Wolf K., Buhk H. -J. 1998; Identification of a protein essential for replication of porcine circovirus. Journal of General Virology 79:381–384
    [Google Scholar]
  24. Meehan B. M., Todd D., Creelan J. L., Earle J. A. P., Hoey E. M., McNulty M. S. 1992; Characterization of viral DNAs from cells infected with chicken anaemia agent : sequence analysis of the cloned replicative form and transfection capabilities of clonedgenome fragments. Archives of Virology 124:301–319
    [Google Scholar]
  25. Meehan B. M., Creelan J. L., McNulty M. S., Todd D. 1997; Sequence of porcine circovirus DNA: affinities with plant circoviruses. Journal of General Virology 78:221–227
    [Google Scholar]
  26. Molitor T. W., Joo H. S., Collet M. S. 1984; Porcine parvovirus DNA: characterisation of the genomic and replicative form DNA of two virus isolates. Virology 137:241–254
    [Google Scholar]
  27. Nayar G. P. S., Hamel A., Lin L. 1997; Detection and characterization of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs. Canadian Veterinary Journal 38:385–386
    [Google Scholar]
  28. Ritchie B. W., Niagro F. D., Lukert P. D., Steffens W. L. III Latimer K. S. 1989; Characterization of a new virus from cockatoos with psittacine beak and feather disease virus. Virology 171:83–88
    [Google Scholar]
  29. Rohde W., Randles J. W., Langridge P., Hanold D. 1990; Nucleotide sequence of a circular single-stranded DNA associated with coconut foliar decay virus. Virology 176:648–651
    [Google Scholar]
  30. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning : A Laboratory Manual Cold Spring Harbor Laboratory, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  31. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  32. Segalés J., Sitjar M., Domingo M., Dee S., DelPozo M., Noval R., Sacristan C., DeLasHeras A., Ferro A., Latimer K. S. 1997; First report of post-weaning multisystemic wasting syndrome in pigs in Spain. Veterinary Record 141:600–601
    [Google Scholar]
  33. Sompayrac L. M., Danna K. J. 1981; Efficient infection of monkey cells with DNA of simian virus 40. Proceedings of the National Academy of Sciences, USA 78:7575–7578
    [Google Scholar]
  34. Tischer I., Buhk H. -J. 1988; Viral DNA from cells infected with porcine circovirus. Zentralblatt fuer Bakteriologie Mikrobiologie und Hygiene series A 270:280–287
    [Google Scholar]
  35. Tischer I., Rasch R., Tochtermann G. 1974; Characterization of papovavirus- and picornavirus-like particles in permanent pig kidney cell lines. Zentralblatt fuer Bakteriologie Mikrobiologie und Hygiene series A 226:153–167
    [Google Scholar]
  36. Tischer I., Gelderblom H., Vettermann W., Koch M. A. 1982; A very small porcine virus with a circular single-stranded DNA. Nature 295:64–66
    [Google Scholar]
  37. Tischer I., Mields W., Wolff D., Vagt M., Griem W. 1986; Studies on the pathogenicity of porcine circovirus. Archives of Virology 91:271–276
    [Google Scholar]
  38. Tischer I., Peters D., Rasch R., Pociuli S. 1987; Replication of porcine circovirus - induction by glucosamine and cell-cycle dependence. Archives of Virology 96:39–57
    [Google Scholar]
  39. Todd D., Creelan J. L., Mackie D. P., Rixon F., McNulty M. S. 1990; Purification and biochemical characterisationof chicken anaemia agent. Journal of General Virology 71:819–823
    [Google Scholar]
  40. Todd D., Creelan J. L., McNulty M. S. 1991a; Dot blot hybridization assay for chicken anemia agent using a cloned DNA probe. Journal of Clinical Microbiology 29:933–939
    [Google Scholar]
  41. Todd D., Niagro F. D., Ritchie B. W., Curran W., Allan G. M., Lukert P. D., Latimer K. S., Steffens W. L. III McNulty M. S. 1991b; Comparison of three animal viruses with circular single-stranded DNA genomes. Archives of Virology 117:129–135
    [Google Scholar]
  42. Todd D., Connor T. J., Calvert V. M., Creelan J. L., Meehan B. M., McNulty M. S. 1995; Molecular cloning of an attenuated chicken anaemia virus isolate following repeated cell culture passage. Avian Pathology 24:171–187
    [Google Scholar]
  43. Todd D., Creelan J. L., Meehan B. M., McNulty M. S. 1996; Investigation of the transfection capabilityof cloned tandemly-repeated chicken anaemia virus DNA fragments. Archives of Virology 141:1523–1534
    [Google Scholar]
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