Potato virus Y group C isolates are a homogeneous pathotype but two different genetic strains Free

Abstract

Potato virus Y group C isolates (PVY) have been characterized according to biological, molecular and genetic criteria. Two genetic strains, PVY and PVY , were identified on the basis of genetic distances (among them and other PVY strains), host range (ability or inability to infect pepper), MAb response (ELISA recognition with MAb 10E3) andcoat protein processing site. Some characteristics, such as aphid transmission and ELISA using other MAbs, did not correlate with classification into these two genetic strains. All isolates tested induced a hypersensitive response on potatoes bearing the resistance gene, confirming the nature of PVY isolates as a homogeneous pathotype.

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1998-08-01
2024-03-29
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References

  1. Aleman-Verdaguer M.-E., Goudou-Urbino C., Dubern J., Beachy R. N., Fauquet C. 1997; Analysis of the sequence diversity of the P1, HC, P3, NIb and CP genomic regions of several yam mosaic potyvirus isolates: implications for the intraspecies molecular diversity of potyviruses. Journal of General Virology 78:1253–1264
    [Google Scholar]
  2. Bawden F. C., Kassanis B. 1947; The behaviour of some naturally occurring strains of potato virus Y. Annals of Applied Biology 34:503–516
    [Google Scholar]
  3. Blanco-Urgoiti B., Ponz F., Martínez A., Marquínez R., Pérez de San Román C., Legorburu J. 1994; Variabilidad molecular en el virus Y de la patata en lotes de patata de siembra en Álava. VII Congreso de la Sociedad Española de Fitopatología Sitges
    [Google Scholar]
  4. Blanco-Urgoiti B., Sánchez F., Dopazo J., Ponz F. 1996; A strain-type clustering of potato virus Y based on the genetic distance between isolates calculated by RFLP analysis of the amplified CP gene. Archives of Virology 141:2425–2442
    [Google Scholar]
  5. Cockerham G. 1970; Genetical studies on resistance to potato viruses X and Y. Heredity 25:309–348
    [Google Scholar]
  6. De Bokx J. A., Huttinga H. 1981; Potato virus Y. CMI/AAB Descriptions of Plant Viruses242
    [Google Scholar]
  7. De Bokx J. A., Piron P. G. M. 1978; Transmission of potato virus YC by aphids. Abstracts of the 7th Triennial Conference of the European Association of Potato Researchers
    [Google Scholar]
  8. Dinant S., Lot H., Albouy J., Kuziak C., Meyer M., Astier-Manifacier S. 1991; Nucleotide sequence of the 3′ terminal region of lettuce mosaic potyvirus RNA shows a Gln/Val dipeptide at the cleavage site between the polymerase and the CP. Archives of Virology 116:235–252
    [Google Scholar]
  9. Fribourg C. E., Nakashima J. 1984; Characterization of a new potyvirus from potato. Phytopathology 74:1363–1369
    [Google Scholar]
  10. GebreSelassie K., Marchoux G., Delecolle B., Pochard E. 1985; Variabilité naturelle des souches du virus Y de la pomme de terre dans les cultures de piment du sud-est de la France. Caracterisation et classification en pathotypes. Agronomie 5:621–630
    [Google Scholar]
  11. Gugerli P., Fries P. 1983; Characterization ofmonoclonal antibodies to potato virus Y and their use for virus detection. Journal of General Virology 64:2471–2477
    [Google Scholar]
  12. Jones R. A. C. 1990; Strain group specific and virus specific hy-persensitive reactions to infection with potyvirus in potato cultivars. Annals of Applied Biology 117:93–105
    [Google Scholar]
  13. MacDonald J. G., Singh R. P. 1996; Host range, symptomology, and serology of isolates of potato virus Y (PVY) that share properties with both the PVYN and the PVYO strain groups. American Potato Journal 73:309–315
    [Google Scholar]
  14. Marrou J. 1967; Amélioration des méthodes de transmission mécanique des virus par absorption des inhibiteurs d’infection sur le charbon végétal. Comptes Rendus de l’Académie d’Agriculture Français 53:972–981
    [Google Scholar]
  15. Nolasco G., De Blas C., Torres V., Ponz F. 1993; A method combining immunocapture and PCR amplification in a microtiter plate for the detection of plant viruses and subviral pathogens. Journal of Virological Methods 45:201–218
    [Google Scholar]
  16. Pérez de San Román C., Legorburu J., Sanz A., Vela C., Cambra M. 1987; Serotyping of PVY and PVX with monoclonal antibodies. Potato Research 30:166
    [Google Scholar]
  17. Revers F., Le Gall O., Candresse T., Le Romancer M., Dunez J. 1996; Frequent occurrence of recombinant potyvirus isolates. Journalof General Virology 77:1953–1965
    [Google Scholar]
  18. Riechmann J. L., Laín S., García J. A. 1992; Highlights and prospects of potyvirus molecular biology. Journal of General Virology 73:1–16
    [Google Scholar]
  19. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  20. Sanz A., Cambra M., Pérez de San Román C., Miguet J. G., Cortes E., Gorris M. T., Vela C. 1990; Preparation of additional monoclonal antibodies for detection and discrimination of potato virus Y isolates infecting potato. Potato Research 33:365–375
    [Google Scholar]
  21. Shukla D. D., Ward C. W., Brunt A. A. 1994 The Potyviridae Wallingford: CAB International;
    [Google Scholar]
  22. Soto M. J., Luis Arteaga M., Fereres A., Ponz F. 1994; Limited degree of serological variability in pepper strains of potato virus Y as revealed by analysis with monoclonal antibodies. Annals of Applied Biology 124:37–43
    [Google Scholar]
  23. Thole V., Dalmay T., Burgyan J., Balázs E. 1993; Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA. Gene 123:149–156
    [Google Scholar]
  24. Ward C. W., Weiller G. F., Shukla D. D., Gibbs A. 1995; Molecular systematics of the Potyviridae, the largest plant virus family. In Molecular Basis of Virus Evolution pp. 477–500 Gibbs A., Garcia Arenal F., Calisher C. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
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