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Volume 79, Issue 8

Genome properties of beet virus Q, a new furo-like virus from sugarbeet, determined from unpurified virus Free

Abstract

Based solely on the information that beet virus Q (BVQ) contains tubular particles, the entire nucleotide sequence of its tripartite genome was determined from unpurified virus in ca. 40 ml crude sap from locally infected A starting sequence for RNA 1 was generated using primers corresponding to highly conserved helicase domains in the respective RNAs of furo-, pomo-, peclu-, hordei- and tobraviruses, and was extended by a walking random-primed cDNA approach. The similarity of the 3′ ends of furoviral RNAs allowed starting sequences for BVQ RNAs 2 and 3 to be obtained once the 3′ end of RNA 1 was known. BVQ RNA 1 encodes a protein with a methyltransferaselike, a variable and a helicase-like region, and for a readthrough protein which, in addition, contains an RNA-dependent RNA polymerase region. RNA 2 carries the coat protein gene, a coat protein read- through protein gene and two additional ORFs which may have arisen by deletions from an originally larger readthrough domain. RNA 3 carries a triple gene block resembling that of several other rod-shaped viruses. The 5′ UTRs of the three RNAs have the potential to form a series of hairpins with C-Aand C-C mismatches resembling those found in tymoviral RNAs. The 3′ ends can be folded into tRNA-like structures which are preceded by a long hairpin-like structure and an upstream pseudoknot domain. BVQ belongs to the recently proposed genus ; it shows evolutionary relationships to furoviruses , peclu-, hordei-, tobra-, tymo-, tobamo-, carla- and potexviruses.

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© Society for General Microbiology 1998
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1998-08-01
2024-04-19
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Genome properties of beet virus Q, a new furo-like virus from sugarbeet, determined from unpurified virus
J. Gen. Virol. 79, 2027 (1998); https://doi.org/10.1099/0022-1317-79-8-2027
/content/journal/jgv/10.1099/0022-1317-79-8-2027
/content/journal/jgv/10.1099/0022-1317-79-8-2027
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