@article{mbs:/content/journal/jgv/10.1099/0022-1317-79-8-1971, author = "Gill, Dilbinder K. and Bible, Jon M. and Biswas, Chandrima and Kell, Barbara and Best, Jennifer M. and Punchard, Neville A. and Cason, John", title = "Proliferative T-cell responses to human papillomavirus type 16 E5 are decreased amongst women with high-grade neoplasia", journal= "Journal of General Virology", year = "1998", volume = "79", number = "8", pages = "1971-1976", doi = "https://doi.org/10.1099/0022-1317-79-8-1971", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-8-1971", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Proliferative responses to human papillomavirus type 16 (HPV-16) E5 peptides were determined for short-term cell lines derived from peripheral blood mononuclear cells of 75 women. Cell lines from 16 of the 75 women proliferated in response to stimulation with pooled E5 peptides; this was most common for patients with low-grade squamous cervical intraepithelial lesions (LSIL; 6 of 15 patients, 40%) and less frequent for asymptomatic women with no cervical lesions (4 of 20, 20%), those with high-grade squamous intraepithelial lesions (HSIL; 5 of 33,15%) and others with cervical cancer (1 of 7, 14%, P= 0·027). Amongst these patients, proliferative responses were exclusive to those that were positive for HPV-16 DNA(12 of 41, 29%; c.f. none of 13 HPV-16 DNA-negative subjects exhibited a proliferative response; P=0·023) and were again most prevalent amongst HPV-16 DNA-positive LSIL (6 of 14, 43%), as compared with HPV-16 DNA-positive HSIL (5 of 23, 22%) or HPV-16 DNA-positive cervical cancer patients (1 of 4, 25%, P > 0·05). In contrast, for asymptomatic women, responsiveness was statistically independent of HPV-16 DNA status, i.e. responsiveness in HPV-16 DNA-positive and DNAnegative subjects was observed in 3 of 15 (20%) and 1 of 5 (20%) cases, respectively (P > 0·05). There were no associations between detection of HPV-16 mRNA and proliferative responses (P > 0·05). These data suggest that HPV-16 E5-specific T-helper activity is depressed amongst women with HSIL lesions.", }