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Volume 79, Issue 8

Proliferative T-cell responses to human papillomavirus type 16 E5 are decreased amongst women with high-grade neoplasia Free

Abstract

Proliferative responses to human papillomavirus type 16 (HPV-16) E5 peptides were determined for short-term cell lines derived from peripheral blood mononuclear cells of 75 women. Cell lines from 16 of the 75 women proliferated in response to stimulation with pooled E5 peptides; this was most common for patients with low-grade squamous cervical intraepithelial lesions (LSIL; 6 of 15 patients, 40%) and less frequent for asymptomatic women with no cervical lesions (4 of 20, 20%), those with high-grade squamous intraepithelial lesions (HSIL; 5 of 33,15%) and others with cervical cancer (1 of 7, 14%, = 0·027). Amongst these patients, proliferative responses were exclusive to those that were positive for HPV-16 DNA(12 of 41, 29%; c.f. none of 13 HPV-16 DNA-negative subjects exhibited a proliferative response; =0·023) and were again most prevalent amongst HPV-16 DNA-positive LSIL (6 of 14, 43%), as compared with HPV-16 DNA-positive HSIL (5 of 23, 22%) or HPV-16 DNA-positive cervical cancer patients (1 of 4, 25%, > 0·05). In contrast, for asymptomatic women, responsiveness was statistically independent of HPV-16 DNA status, i.e. responsiveness in HPV-16 DNA-positive and DNAnegative subjects was observed in 3 of 15 (20%) and 1 of 5 (20%) cases, respectively ( > 0·05). There were no associations between detection of HPV-16 mRNA and proliferative responses ( > 0·05). These data suggest that HPV-16 E5-specific T-helper activity is depressed amongst women with HSIL lesions.

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© Society for General Microbiology 1998
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1998-08-01
2024-04-19
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Proliferative T-cell responses to human papillomavirus type 16 E5 are decreased amongst women with high-grade neoplasia
J. Gen. Virol. 79, 1971 (1998); https://doi.org/10.1099/0022-1317-79-8-1971
/content/journal/jgv/10.1099/0022-1317-79-8-1971
/content/journal/jgv/10.1099/0022-1317-79-8-1971
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