1887

Abstract

The Vif protein of human immunodeficiency virus type 1 (HIV-1) is essential for the infectivity of virions produced by non-permissive cells. The primary replicative defect of Vif particles involves either synthesis or stability of viral DNA, but the mechanism of this defect is unknown. Here, we report the results of a detailed analysis of HIV-1 DNA synthesis by isogenic Vifmutants produced by different chronically infected H9 clones, which exhibit different degrees of impairment in their replicative capacity. We found that the degree of impairment of DNA synthesis by the mutant particles always correlated with the degree of their loss of infectivity. This impairment appears to be global, with a defect increasing along with synthesis of longer viral DNA species. We conclude that the primary replicative defect of Vif virus involves the capacity of the reverse transcription complex of HIV-1 to efficiently elongate viral DNA, resulting in an inability to produce full-length viral DNA genomes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-8-1945
1998-08-01
2022-05-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/8/9714242.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-8-1945&mimeType=html&fmt=ahah

References

  1. Borman A. M., Quillent C., Charneau P., Dauguet C., Clavel F. 1995; Human immunodeficiency virus type 1 Vif mutant particles from restrictive cells : role of Vif in correct particle assembly and infectivity. Journal of Virology 69:2058–2067
    [Google Scholar]
  2. Bouyac M., Rey F., Nascimbeni M., Courcoul M., Sire J., Blanc D., Clavel F., Vigne R., Spire B. 1997; Phenotypically Vif human immunodeficiency virus type 1 is produced by chronically infected restrictive cells. Journal of Virology 71:2473–2477
    [Google Scholar]
  3. Camaur D., Trono D. 1996; Characterization of human immuno-deficiency virus type 1 Vif particle incorporation. Journal of Virology 70:6106–6111
    [Google Scholar]
  4. Chakrabarti L., Guyader M., Alizon M., Daniel M. D., Desrosiers R. C., Tiollais P., Sonigo P. 1987; Molecular cloning and nucleotide sequence of simian immunodeficiency virus from macaques. Nature 328:543–547
    [Google Scholar]
  5. Courcoul M., Patience C., Rey F., Blanc D., Harmache A., Sire J., Vigne R., Spire B. 1995; Peripheral blood mononuclear cells produce normal amounts of defective Vif human immunodeficiency virus type 1 particles which are restricted for the preretrotranscription steps. Journal of Virology 69:2068–2074
    [Google Scholar]
  6. Gabuzda D., Lawrence K., Langhoff E., Terwilliger E., Dorfman T., Haseltine W., Sodroski J. 1992; Role of Vif in replication of HIV type 1 in CD4+ T lymphocytes. Journal of Virology 66:6489–6495
    [Google Scholar]
  7. Gonsalves J., Jallepalli P., Gabuzda D. 1994; Subcellular localization of the Vif protein of human immunodeficiency virus type-1. Journal of Virology 68:704–712
    [Google Scholar]
  8. Gonçalves J., Korin Y., Zack J., Gabuzda D. 1996; Role of Vif in human immunodeficiency virus type 1 reverse transcription. Journal of Virology 70:8701–8709
    [Google Scholar]
  9. Guyader M., Emerman M., Sonigo P., Clavel F., Montagnier L., Alizon M. 1987; Genetic organization and transactivation of human immunodeficiency virus type 2. Nature 326:662–669
    [Google Scholar]
  10. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  11. Höglund S., Öhagen A., Lawrence K., Gabuzda D. 1994; Role of Vif during packing of the core of HIV-1. Virology 201:349–355
    [Google Scholar]
  12. Karczewski M., Strebel K. 1996; Cytoskeleton association and virion incorporation of the human immunodeficiency virus type 1 Vif protein. Journal of Virology 70:494–507
    [Google Scholar]
  13. Liu H., Wu X., Newman M., Shaw G. M., Hahn B. H., Kappes J. C. 1995; The Vif protein of human and simian immunodeficiency viruses is packaged into virions and associates with viral core structures. Journal of Virology 69:7630–7638
    [Google Scholar]
  14. Oberste M., Gonda M. 1992; Conservation of amino-acid sequence motifs in lentivirus Vif proteins. Virus Genes 6:95–102
    [Google Scholar]
  15. Sakai H., Shibata R., Sakuragi J.-I., Sakuragi S., Kawamura M., Adachi A. 1993; Cell-dependent requirement of human immunodeficiency virus type 1 Vif protein for maturation of virus particles. Journal of Virology 67:1663–1666
    [Google Scholar]
  16. Simon J. H. M., Malim M. H. 1996; The human immunodeficiency virus type 1 Vif protein modulates the postpenetration stability of viral nucleoprotein complexes. Journal of Virology 70:5297–5305
    [Google Scholar]
  17. Sonigo P., Alizon M., Staskus K., Klatzmann D., Cole S., Danos O., Retzel E., Tiollais P., Haase A., Wain-Hobson S. 1985; Nucleotide sequence of the Visna lentivirus: relationship to the AIDS virus. Cell 43:369–382
    [Google Scholar]
  18. Sova P., Volsky D. 1993; Efficiency of viral DNA synthesis during infection of permissive and non-permissive cells with Vifnegative HIV-1. Journal of Virology 67:6322–6326
    [Google Scholar]
  19. Von Schwedler U., Song J., Aiken C., Trono D. 1993; Vif is crucial for human immunodeficiency virus type 1 proviral DNA synthesis in infected cells. Journal of Virology 67:4945–4955
    [Google Scholar]
  20. Wain-Hobson S., Sonigo P., Danos O., Cole S., Alizon M. 1985; Nucleotide sequence of the AIDS virus, LAV. Cell 40:9–17
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-8-1945
Loading
/content/journal/jgv/10.1099/0022-1317-79-8-1945
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error