1887

Abstract

We previously found two cell lines (MT-2 and PH5CH) that were susceptible to hepatitis C virus (HCV) infection. Analysis of the infectivity of sera from HCV-positive blood donors for MT-2 and PH5CH cells suggested the cell tropism of HCV. To investigate further the cell tropism of HCV, the dynamics of HCV populations during culture were examined using three MT-2 clones and three PH5CH clones, infected with inoculum 1B-2. To type HCV populations in these infected cells, the HCV hypervariable region 1 (HVR1) in these cloned cells was characterized by sequence analysis and II digestion analysis, which could distinguish three major HVR1 types (I, II and III) derived from the inoculum 1B-2. It was found that genomes containing HVR1 type I became predominant in MT-2 clones, and genomes containing HVR1 type II became predominant in PH5CH clones during culture after inoculation. These results suggest that inoculum 1B-2 contains both lymphotropic and hepatotropic HCV species, which can be distinguished by HVR1 type. To search for cell type- specific sequences in regions other than HVR1, three HCV cDNA clones (3·4 kb of the 5′ noncoding region to the nonstructural 2 region) containing HVR1 type I obtained from HCV-infected MT-2C cells, and three HCV cDNA clones containing HVR1 type II obtained from HCV-infected PH5CH7 cells were sequenced. Following a comparison of the sequences, 11 amino acids were identified as candidates for determinants of the cell tropism of HCV.

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1998-08-01
2022-08-17
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References

  1. Carrillo A., Trowbridge D. B., Westervelt P., Ratner L. 1993; Identification of HIV1 determinants for T lymphoid cell line infection. Virology 197:817–824
    [Google Scholar]
  2. Choo Q. L., Kuo G., Weiner A. J., Overby L. R., Bradley D. W., Houghton M. 1989; Isolation of a cDNA clone derived from a blood- born non-A, non-B viral hepatitis genome. Science 244:359–362
    [Google Scholar]
  3. Cribier B., Schmitt C., Bingen A., Kirn A., Keller F. 1995; In vitro infection of peripheral blood mononuclear cells by hepatitis C virus. Journal of General Virology 76:2485–2491
    [Google Scholar]
  4. Dash S., Halim A.-B., Tsuji H., Hiramatsu N., Gerber M. A. 1997; Transfection of HepG2 cells with infectious hepatitis C virus genome. American Journal of Pathology 151:363–373
    [Google Scholar]
  5. Day S. P., Murphy P., Brown A., Lemon S. M. 1992; Mutations within the 5′ nontranslated region of hepatitis A virus RNA which enhance replication in BS-C-1 cells. Journal of Virology 66:6533–6540
    [Google Scholar]
  6. Ferri C., Monti M., Civita L. L., Longombardo G., Greco F., Pasero G., Gentilini P., Bombardieri S., Zignego A. L. 1993; Infection of peripheral blood mononuclear cells by hepatitis C virus in mixed cryoglobulinemia. Blood 82:3701–3704
    [Google Scholar]
  7. Ferri C., Civita L. L., Zignego A. L., Pasero G. 1997; Hepatitis-C-virus infection and cancer. International Journal of Cancer 71:1113–1115
    [Google Scholar]
  8. Gabrielli A., Manzin A., Caniglia M. L., Paolucci S., Danieli M. G., Clementi M. 1994; Active hepatitis C virus infection in bone marrow and peripheral blood mononuclear cells from patients with mixed cryoglobulinemia. Clinical and Experimental Immunology 97:87–93
    [Google Scholar]
  9. Gunji T., Kato N., Hijikata M., Hayashi K., Saitoh S., Shimotohno K. 1994; Specific detection of positive and negative stranded hepatitis C viral RNA using chemical RNA modification. Archives of Virology 134:293–302
    [Google Scholar]
  10. Hijikata M., Kato N., Ootsuyama Y., Nakagawa M., Shimotohno K. 1991a; Gene mapping of the putative structural region of the hepatitis C virus genome by in vitro processing analysis. Proceedings of the National Academy of Sciences, USA 88:5547–5551
    [Google Scholar]
  11. Hijikata M., Kato N., Ootsuyama Y., Nakagawa M., Ohkoshi S., Shimotohno K. 1991b; Hypervariable regions in the putative glycoprotein of hepatitis C virus. Biochemical and Biophysical Research Communications 175:220–228
    [Google Scholar]
  12. Hijikata M., Mizushima H., Akagi T., Mori S., Kakiuchi N., Kato N., Tanaka T., Kimura K., Shimotohno K. 1993a; Two distinct proteinase activities required for the processing of a putative nonstructural protein of hepatitis C virus. Journal of Virology 67:4665–4675
    [Google Scholar]
  13. Hijikata M., Mizushima H., Tanji Y., Komoda Y., Hirowatari Y., Akagi T., Kato N., Kimura K., Shimotohno K. 1993b; Proteolytic processing and membrane association of putative nonstructural proteins of hepatitis C virus. Proceedings of the National Academy of Sciences, USA 90:10773–10777
    [Google Scholar]
  14. Hijikata M., Mizuno K., Rikihisa T., Shimizu Y. K., Iwamoto A., Nakajima N., Yoshikura H. 1995; Selective transmission of hepatitis C virus in vivo and in vitro. Archives of Virology 140:1623–1628
    [Google Scholar]
  15. Hwang S. S., Boyle T. J., Lyerly H. K., Cullen B. R. 1991; Identification of the envelope V3 loop as the primary determinant of cell tropism in HIV-1. Science 253:71–74
    [Google Scholar]
  16. Ikeda M., Kato N., Mizutani T., Sugiyama K., Tanaka K., Shimotohno K. 1997; Analysis of cell tropism of HCV by using in vitro HCV-infected human lymphocytes and hepatocytes. Journal of Hepatology 27:445–454
    [Google Scholar]
  17. Ikeda M., Sugiyama K., Mizutani T., Tanaka T., Tanaka K., Sekihara H., Shimotohno K., Kato N. 1998; Human hepatocyte clonal cell lines that support persistent replication of hepatitis C virus. Virus Research in press
    [Google Scholar]
  18. Ito T., Mukaigawa J., Zuo J., Hirabayashi Y., Mitamura K., Yasui K. 1996; Cultivation of hepatitis C virus in primary hepatocyte culture from patients with chronic hepatitis C results in release of high titre infectious virus. Journal of General Virology 77:1043–1054
    [Google Scholar]
  19. Kato N., Hijikata M., Ootsuyama Y., Nakagawa M., Ohkoshi S., Shimotohno K. 1990a; Sequence diversity of hepatitis C viral genomes. Molecular Biology & Medicine 7:495–501
    [Google Scholar]
  20. Kato N., Hijikata M., Ootsuyama Y., Nakagawa M., Ohkoshi S., Sugimura T., Shimotohno K. 1990b; Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis. Proceedings of the National Academy of Sciences, USA 87:9524–9528
    [Google Scholar]
  21. Kato N., Ootsuyama Y., Ohkoshi S., Nakazawa T., Mori S., Hijikata M., Shimotohno K. 1991; Distribution of plural HCV types in Japan. Biochemical and Biophysical Research Communications 181:279–285
    [Google Scholar]
  22. Kato N., Ootsuyama Y., Ohkoshi S., Nakazawa T., Sekiya H., Hijikata M., Shimotohno K. 1992a; Characterization of hypervariable regions in the putative envelope protein of hepatitis C virus. Biochemical and Biophysical Research Communications 189:119–127
    [Google Scholar]
  23. Kato N., Ootsuyama Y., Tanaka T., Nakagawa M., Nakazawa T., Muraiso K., Ohkoshi S., Hijikata M., Shimotohno K. 1992b; Marked sequence diversity in the putative envelope proteins of hepatitis C viruses. Virus Research 22:107–123
    [Google Scholar]
  24. Kato N., Sekiya H., Ootsuyama Y., Nakazawa T., Hijikata M., Ohkoshi S., Shimotohno K. 1993; Humoral immune response to hypervariable region 1 of the putative envelope glycoprotein (gp70) of hepatitis C virus. Journal of Virology 67:3923–3930
    [Google Scholar]
  25. Kato N., Nakazawa T., Ootsuyama Y., Sugiyama K., Ohkoshi S., Shimotohno K. 1994a; Virus isolate-specific antibodies against hypervariable region 1 of the hepatitis C virus second envelope protein, gp70. Japanese Journal of Cancer Research 85:987–991
    [Google Scholar]
  26. Kato N., Ootsuyama Y., Sekiya H., Ohkoshi S., Nakazawa T., Hijikata M., Shimotohno K. 1994b; Genetic drift in hypervariable region 1 of the viral genome in persistent hepatitis C virus infection. Journal of Virology 68:4776–4784
    [Google Scholar]
  27. Kato N., Nakazawa T., Mizutani T., Shimotohno K. 1995; Susceptibility of human T-lymphotropic virus type I infected cell line MT-2 to hepatitis C virus infection. Biochemical and Biophysical Research Communications 206:606–614
    [Google Scholar]
  28. Kato N., Ikeda M., Mizutani T., Sugiyama K., Noguchi M., Hirohashi S., Shimotohno K. 1996; Replication of hepatitis C virus in cultured non-neoplastic human hepatocytes. Japanese Journalof Cancer Research 87:787–792
    [Google Scholar]
  29. Kawamura N., Kohara M., Abe S., Komatsu T., Tago K., Arita M., Nomoto A. 1989; Determinants in the 5′ noncoding region of poliovirus sabin 1 RNA that influence the attenuation phenotype. Journal of Virology 63:1302–1309
    [Google Scholar]
  30. Koito A., Stamatatos L., Cheng-Mayer C. 1995; Small amino acid sequence changes within the V2 domain can affect the function of a T-cell line-tropic human immunodeficiency virus type 1 envelope gp120. Virology 206:878–884
    [Google Scholar]
  31. Kolykhalov A. A., Agapov E. V., Blight K. J., Mihalik K., Feinstone S. M., Rice C. M. 1997; Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA. Science 277:570–574
    [Google Scholar]
  32. Kuo G., Choo Q. L., Alter H. J., Gitnick G. L., Redwker A. G., Purcell R. H., Miyamura T., Dienstag J. L., Alter M. J., Stevens C. E., Tegtmeier G. E., Bonino F., Colombo W. S., Lee W. S., Kuo C., Berger K., Shuster J. R., Overby L. R., Bradley D. W., Houghton M. 1989; An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 244:362–364
    [Google Scholar]
  33. Lanford R. E., Sureau C., Jaco J. R., White R., Fuerst T. R. 1994; Demonstration of in vitro infection of chimpanzee hepatocytes with hepatitis C virus using strand-specific RT/PCR. Virology 202:606–614
    [Google Scholar]
  34. Lerat H., Berby F., Trabaud M. A., Vidalin O., Major M., Trepo C., Inchauspe G. 1996; Specific detection of hepatitis C virus minus strand RNA in hematopoietic cells. Journal of Clinical Investigation 97:845–851
    [Google Scholar]
  35. Luppi M., Ferrari M. G., Bonaccorcio G., Longo G., Narni F., Barozzi P., Marasca R., Mussini C., Torelli G. 1996; Hepatitis C virus infection in subsets of neoplastic lymphoproliferations not associated with cryoglobulinemia. Leukemia 10:351–355
    [Google Scholar]
  36. McOmish F., Chan S.-W., Dow B. C., Gillon J., Frame W. D., Crawford R. J., Yap P. L., Follett E. A. C., Simmonds P. 1993; Detection of three types of hepatitis C virus in blood donors: investigation of type-specific differences in serological reactivity and rate of alanine aminotransferase abnormalities. Transfusion 33:7–13
    [Google Scholar]
  37. Mizutani T., Kato N., Masanori I., Sugiyama K., Shimotohno K. 1996a; Long-term human T cell culture system supporting hepatitis C virus replication. Biochemical and Biophysical Research Communications 277:822–826
    [Google Scholar]
  38. Mizutani T., Kato N., Saito S., Ikeda M., Sugiyama K., Shimotohno K. 1996b; Characterization of hepatitis C virus replication in cloned cells obtained from a human T-cell leukemia virus type 1-infected cell line, MT-2. Journal of Virology 70:7219–7223
    [Google Scholar]
  39. Müller H. M., Pfaff E., Goeser T., Kallinowski B., Solbach C., Theilmann L. 1993; Peripheral blood leukocytes serve as a possible extrahepatic site for hepatitis C virus replication. Journal of General Virology 74:669–676
    [Google Scholar]
  40. Nakajima N., Hijikata M., Yoshikura H., Shimizu Y. K. 1996; Characterization of long-term cultures of hepatitis C virus. Journal of Virology 70:3325–3329
    [Google Scholar]
  41. Nakao T., Enomoto N., Takada N., Takada A., Date T. 1991; Typing of hepatitis C virus genomes by restriction fragment length polymorphism. Journal of General Virology 72:2105–2112
    [Google Scholar]
  42. Nei M., Gojobori T. 1986; Simple method for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Molecular Biology and Evolution 3:418–426
    [Google Scholar]
  43. Noguchi M., Hirohashi S. 1996; Cell lines from non-neoplastic liver and hepatocellular carcinoma tissue from a single patient. In Vitro Cellular & Developmental Biology Animal 32:135–137
    [Google Scholar]
  44. Ohkoshi S., Kojima H., Tawaraya H., Miyajima T., Kamimura T., Asakura H., Satoh A., Hirose S., Hijikata M., Kato N., Shimotohno K. 1990; Prevalence of antibody against non-A, non-B hepatitis virus in Japanese patients with hepatocellular carcinoma. Japanese Journal of Cancer Research 81:550–552
    [Google Scholar]
  45. Saito I., Miyamura T., Ohbayashi A., Harada H., Katayama T., Kikuchi S., Watanabe Y., Koi S., Onji M., Ohta Y., Choo Q. L., Houghton M., Kuo G. 1990; Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proceedings of the National Academy of Sciences, USA 87:6547–6549
    [Google Scholar]
  46. Saito S., Kato N., Hijikata M., Gunji T., Itabashi M., Kondo M., Tanaka K., Shimotohno K. 1996; Comparison of hypervariable regions (HVR1 and HVR2) in positive- and negative-stranded hepatitis C virus RNA in cancerous and noncancerous liver tissue, peripheral blood mononuclear cells, and serum from a patient with hepatocellular carcinoma. International Journal of Cancer 67:199–203
    [Google Scholar]
  47. Seipp S., Mueller H. M., Pfaff E., Stremmel W., Theilmann L., Goeser T. 1997; Establishment of persistent hepatitis C virus infection and replication in vitro. Journal of General Virology 78:2467–2476
    [Google Scholar]
  48. Sekiya H., Kato N., Ootsuyama Y., Nakazawa T., Yamauchi K., Shimotohno K. 1994; Genetic alterations of the putative envelope proteins encoding region of the hepatitis C virus in the progression to relapsed phase from acute hepatitis : humoral immune response to hypervariable region 1. International Journal of Cancer 57:664–670
    [Google Scholar]
  49. Shimizu Y. K., Yoshikura H. 1994; Multicycle infection of hepatitis C virus in cell culture and inhibition by alpha and beta interferons. Journal of Virology 68:8406–8408
    [Google Scholar]
  50. Shimizu Y. K., Iwamoto A., Hijikata M., Purcell R. H., Yoshikura H. 1992; Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line. Proceedings of the National Academy of Sciences, USA 89:5477–5481
    [Google Scholar]
  51. Shimizu Y. K., Purcell R. H., Yoshikura H. 1993; Correlation between the infectivity of hepatitis C virus in vivo and its infectivity in vitro. Proceedings of theNational Academy of Sciences, USA 70:6037–6041
    [Google Scholar]
  52. Shimizu Y. K., Igarashi H., Kanematu T., Fujiwara K., Wong D. C., Purcell R. H., Yoshikura H. 1997; Sequence analysis of the hepatitis C virus genome recovered from serum, liver, and peripheral blood mononuclear cells of infected chimpanzees. Journal of Virology 71:5769–5773
    [Google Scholar]
  53. Shioda T., Oka S., Ida S., Nokihara K., Toriyoshi H., Mori S., Takebe Y., Kimura S., Shimada K., Nagai Y. 1994; A naturally occurring single basic amino acid substitution in the V3 region of the human immunodeficiency virus type 1 env protein alters the cellular host range and antigenic structure of the virus. Journal of Virology 68:7689–7696
    [Google Scholar]
  54. Silvestri F., Pipan C., Barillari G., Zaja F., Fanin R., Infanti L., Russo D., Falasca E., Botta G. A., Baccarani M. 1996; Prevalence of hepatitis C virus infection in patient with lymphoproliferative disorder. Blood 87:4296–4301
    [Google Scholar]
  55. Sugiyama K., Kato N., Ikeda M., Mizutani T., Shimotohno K., Kato T., Sugiyama Y., Hasumi K. 1997a; Hepatitis C virus in pelvic lymph nodes and female reproductive organs. Japanese Journal of Cancer Research in press
    [Google Scholar]
  56. Sugiyama K., Kato N., Mizutani T., Ikeda M., Tanaka T., Shimotohno K. 1997b; Genetic analysis of the hepatitis C virus (HCV) genome from HCV-infected human T cells. Journal of General Virology 78:329–336
    [Google Scholar]
  57. Takamizawa A., Mori C., Fuki I., Manabe S., Murakami S., Fujita J., Onishi E., Andoh T., Yoshida I., Okayama H. 1991; Structure and organization of the hepatitis C virus genome isolated from human carriers. Journal of Virology 65:1105–1113
    [Google Scholar]
  58. Takeuchi Y., Akutsu M., Murayama K., Shimizu N., Hoshino H. 1991; Host range mutant of human immunodeficiency virus type 1: modification of cell tropism by a single point mutation at the neutralization epitope in the env gene. Journal of Virology 65:1710–1718
    [Google Scholar]
  59. Tanaka T., Kato N., Cho M. J., Shimotohno K. 1995; A novel sequence found at the 3′ terminus of hepatitis C virus genome. Biochemical & Biophysical Research Communications 215:744–749
    [Google Scholar]
  60. Tsukiyama-Kohara K., Iizuka N., Kohara M., Nomoto A. 1992; Internal ribosome entry site within hepatitis C virus RNA. Journal of Virology 66:1476–1483
    [Google Scholar]
  61. Weiner A. J., Brauer M. J., Rosenblatt J., Richman K. H., Tung J., Crawford K., Bonino F., Saracco G., Choo Q. L., Houghton M., Han J. 1991; Variable and hypervariable domains are found in the regions of HCV corresponding to the flavivirus envelope and NS1 proteins and the pestivirus envelope glycoproteins. Virology 180:842–848
    [Google Scholar]
  62. Yanagi M., Purcell R. H., Emerson S. U., Bukh J. 1997; Transcripts from a single full-length cDNA clone of hepatitis C virus are infectious when directly transfected into the liver of a chimpanzee. Proceedings of the National Academy of Sciences, USA 94:8738–8743
    [Google Scholar]
  63. Zibert A., Schreier E., Roggendorf M. 1995; Antibodies in human sera specific to hypervariable region 1 of hepatitis C virus can block viral attachment. Virology 208:653–661
    [Google Scholar]
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