1887

Abstract

Purified virions of bamboo mosaic potexvirus-V isolate (BaMV-V) were found to contain three major RNA species, the 6·4 kb genomic RNA and two RNAs of 2·0 and 1·0 kb, in addition to associated satellite RNA (0·85 kb). Results of Northern blot hybridization, primer extension analysis and cDNA sequencing showed that the packaged 2·0 and 10 kb RNAs of BaMV-V were subgenomic RNAs. In contrast, in the BaMV-O isolate, only genomic RNA was packaged and encapsidated subgenomic RNAs were not detectable. The transcription initiation sites for the 2·0 and 1·0 kb subgenomic RNAs of BaMV-V were located 11 and 16 nt upstream of the initiation codon of open reading frames (ORFs) 2 and 5, respectively. The 2·0 and 1·0 kb subgenomic RNAs functioned as messengers for the ORF2 protein and capsid protein, respectively. Packaging of the 1·0 kb subgenomic RNAs resulted in the formation of rodshaped particles about 70 nm in length. Our results indicate that BaMV isolates have evolved distinctly for packaging of subgenomic RNAs.

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1998-07-01
2024-03-28
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References

  1. AbouHaidar M. 1981; Polar assembly of clover yellow mosaic virus. Journal of General Virology 57:199–203
    [Google Scholar]
  2. Ahlquist P., Luckow V., Kaesberg P. 1981; Complete nucleotide sequence of brome mosaic virus RNA 3. Journal of Molecular Biology 153:23–38
    [Google Scholar]
  3. Bancroft J. B., Rouleau M., Johnston R., Prins L., Mackie G. A. 1991; The entire nucleotide sequence of foxtail mosaic virus RNA. Journal of General Virology 72:2173–2181
    [Google Scholar]
  4. Beck D. L., Guilford P. J., Voot D. M., Andersen M. T., Forster R. L. 1991; Triple gene block proteins of white clover mosaic potexvirus are required for transport. Virology 183:695–702
    [Google Scholar]
  5. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  6. Brederode F. T., Koper-Zwarthoff E. C., Bol J. F. 1980; Complete nucleotide sequence of alfalfa mosaic virus RNA 4. Nucleic Acids Research 8:2213–2223
    [Google Scholar]
  7. Chang B. Y., Lin N. S., Liou D. Y., Chen J. P., Liou G. G., Hsu Y. H. 1997; Subcellular localization of the 28 kDa protein of the triplegene-block of bamboo mosaic potexvirus. Journal of General Virology 78:1175–1179
    [Google Scholar]
  8. Dawson W. O., Lehto K. M. 1990; Regulat ion of tobamovirus gene expression. Advances in Virus Research 38:307–342
    [Google Scholar]
  9. Frolova E., Frolov I., Schlesinger S. 1997; Packaging signals in alphaviruses. Journal of Virology 71:248–258
    [Google Scholar]
  10. Gargouri R., Joshi R. L., Bol J. F., Astier-Manifacier S., Haenni A.-L. 1989; Mechanism of synthesis of turnip yellow mosaic virus coat protein subgenomic RNA in vivo . Virology 171:386–393
    [Google Scholar]
  11. Geigenmuller-Gnirke U., Nitschko H., Schlesinger S. 1993; Deletion analysis of the capsid protein of Sindbis virus: identification of the RNA binding region. Journal of Virology 67:1620–1626
    [Google Scholar]
  12. Gould A. R., Symons R. H. 1982; Cucumber mosaic virus RNA 3. European Journal of Biochemistry 126:217–226
    [Google Scholar]
  13. Guilley H., Briand J. P. 1978; Nucleotide sequence of turnip yellow mosaic coat protein mRNA. Cell 15:113–122
    [Google Scholar]
  14. Kao C. C., Sun J. H. 1996; Initiation of minus-strand RNA synthesis by the brome mosaic virus RNA-dependent RNA polymerase : use of oligonucleotide primers. Journal of Virology 70:6826–6830
    [Google Scholar]
  15. Kim K. H., Hemenway C. 1996; The 5ʹ nontranslated region of potato virus X RNA affects both genomic and subgenomic RNA synthesis. Journal of Virology 70:5533–5540
    [Google Scholar]
  16. Kim K. H., Hemenway C. 1997; Mutations that alter a conserved element upstream of the potato virus X triple block and coat protein genes affect subgenomic RNA accumulation. Virology 232:187–197
    [Google Scholar]
  17. Lin N. S., Chen C. C. 1991; Association of bamboo mosaic virus (BaMV) and BaMV-specific electron-dense crystalline bodies with chloroplasts. Phytopathology 81:1551–1555
    [Google Scholar]
  18. Lin N. S., Hsu Y. H. 1994; A satellite RNA associated with bamboo mosaic potexvirus. Virology 202:707–714
    [Google Scholar]
  19. Lin N. S., Lin F. Z., Huang T. Y., Hsu Y. H. 1992; Genome properties of bamboo mosaic virus. Phytopathology 82:731–734
    [Google Scholar]
  20. Lin N. S., Chen C. C., Hsu Y. H. 1993; Post-embedding in situ hybridization for localization of viral nucleic acid in ultra-thin sections. Journal of Histochemistry and Cytochemistry 41:1513–1519
    [Google Scholar]
  21. Lin N. S., Lin B. Y., Lo N. W., Hu C. C., Chow T. Y., Hsu Y. H. 1994; Nucleotide sequence of the genomic RNA of bamboo mosaic potexvirus. Journal of General Virology 75:2513–2518
    [Google Scholar]
  22. Lin N. S., Lee Y. S., Lin B. Y., Lee C. W., Hsu Y. H. 1996; The open reading frame of bamboo mosaic potexvirus satellite RNA is not essential for its replication and can be replaced with a bacterial gene. Proceedings of the National Academy of Sciences, USA 93:3138–3142
    [Google Scholar]
  23. Maia I. G., Seron K., Haenni A.-L., Bernardi F. 1996; Gene expression from viral RNA genomes. Plant Molecular Biology 32:367–391
    [Google Scholar]
  24. Miller W. A., Dreher T. W., Hall T. C. 1985; Synthesis of brome mosaic virus subgenomic RNA in vitro by internal initiation on (—)-sense genomic RNA. Nature 313:68–70
    [Google Scholar]
  25. Owen K. E., Kuhn R. J. 1996; Identification of a region in the Sindbis virus nucleocapsid protein that is involved in specificity of RNA encapsidation. Journal of Virology 70:2757–2763
    [Google Scholar]
  26. Price M. 1993; Multipartite nature of potato virus χ. Journal of Virology 67:596–600
    [Google Scholar]
  27. Rumenapf T., Strauss E. G., Strauss J. H. 1994; Subgenomic mRNA of aura alphavirus is packaged into virions. Journal of Virology 68:56–62
    [Google Scholar]
  28. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  29. Short M. N., Davies J. W. 1983; Narcissus mosaic virus : a potexvirus with an encapsidated subgenomic message RNA for coat protein. Bioscience Reports 3:837–846
    [Google Scholar]
  30. Sit T. L., Leclerc D., Abouhaidar M. G. 1994; The minimal 5ʹ sequence for in vitro initiation of papaya mosaic potexvirus assembly. Virology 199:238–242
    [Google Scholar]
  31. Skryabin K. G., Morozov S. Yu., Kraev A. S., Rozanov M. N., Chernov B. K., Lukasheva L. I., Atabekov J. G. 1988; Conserved and variable elements in RNA genomes of potexviruses. FEBS Letters 240:33–40
    [Google Scholar]
  32. Solovyev A. G., Novikov V. K., Merits A., Savenkov E. I., Zelenina D. A., Tyulkina L. G., Morozov S. Yu. 1994; Genome characterization and taxonomy of Plantago asiatica mosaic potexvirus. Journal of General Virology 75:259–267
    [Google Scholar]
  33. White K. A., Mackie G. A. 1990; Control and expression of 3ʹ open reading frames in clover yellow mosaic virus. Virology 179:576–584
    [Google Scholar]
  34. White K. A., Bancroft J. B., Mackie G. A. 1992; Mutagenesis of a hexanucleotide sequence conserved in potexvirus RNAs. Virology 189:817–820
    [Google Scholar]
  35. Yang C. C., Liu J. S., Lin C. P., Lin N. S. 1997; Nucleotide sequence and phylogenetic analysis of a bamboo mosaic potexvirus isolate from common bamboo (Bambusa vulgaris McClure). Botanical Bulletin of Academia Sinica 38:77–84
    [Google Scholar]
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