1887

Abstract

A ‘west-Western ’ blotting procedure indicates that adenovirus core protein V is linked to the capsid via protein VI. Double-labelling techniques employing confocal microscopy and immunofluorescence suggest that this linkage is disrupted following infection and penetration of the host cell. Protein V enters the nucleus presumably still in association with the other core proteins attached to the virus genome. Later in infection protein V rapidly accumulates in the nucleus in close association with nucleoli.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-7-1671
1998-07-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/7/9680130.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-7-1671&mimeType=html&fmt=ahah

References

  1. Bergelson J. M., Cunningham J. A., Droguett G., Kurt-Jones E. A., Krithivas A., Hong J. S., Horwitz M. S., Crowell R. L., Finberg R. W. 1997; Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5. Science 275:1320–1323
    [Google Scholar]
  2. Chatterjee P. K., Vayda M. E., Flint S. J. 1985; Interactions between the three adenovirus core proteins. Journal of Virology 55:379–386
    [Google Scholar]
  3. Dales S., Chardonnet Y. 1973; Early events in the interactions of adenoviruses with HeLa cells. IV. Association with microtubules and the nuclear pore complex during vectorial movement of the inoculum. Virology 56:465–483
    [Google Scholar]
  4. Greber V. F., Willetts M., Webster P., Helenus A. 1993; Stepwise dismantling of adenovirus 2 during entry into cells. Cell 75:477–486
    [Google Scholar]
  5. Greber V. F., Webster P., Weber J., Helenius A. 1996; The role of the adenovirus protease in virus entry into cells. EMBO Journal 15:1766–1777
    [Google Scholar]
  6. Hayashi K., Russell W. C. 1968; A study of the development of adenovirus antigens by the immunofluorescent technique. Virology 34:470–480
    [Google Scholar]
  7. Hong S. S., Karayan L., Taurrier J., Curiel D. T., Boulanger P. 1997; Adenovirus type 5 fiber knob binds to the MHC Class I alpha- 2 domain at the surface of human epithelial and B lymphoblastoid cells. EMBO Journal 14:4714–4727
    [Google Scholar]
  8. Kreivi J. P., Akusjarvi G. 1994; Regulation of adenovirus alternative splicing at the level of commitment complex formation. Nucleic Acids Research 2:332–337
    [Google Scholar]
  9. Leith I. R., Hay R. T., Russell W. C. 1989; Adenovirus subviral particles and cores can support limited DNA replication. Journal ofGeneral Virology 70:3235–3248
    [Google Scholar]
  10. Lutz P., Puvion-Dutilleul F., Lutz Y., Kedinger C. 1996; Nucleoplasmic and nucleolar distribution of the adenovirus IVα2ʹ gene product. Journal of Virology 70:3449–3460
    [Google Scholar]
  11. Matthews D. A., Russell W. C. 1994; Adenovirus protein-protein interactions: hexon and protein VI. Journal of General Virology 75:3365–3374
    [Google Scholar]
  12. Matthews D. A., Russell W. C. 1995; Adenovirus protein-protein interactions : molecular parameters governing the binding of protein VI to hexon and the activation of the adenovirus 23K protease. Journal of General Virology 76:1959–1969
    [Google Scholar]
  13. Matthews D. A., Russell W. C. 1998; Adenovirus core protein V interacts with p32 - a protein which is associated with both the mitochondria and the nucleus. Journal of General Virology 79:1677–1685
    [Google Scholar]
  14. Mears W. E., Lam V., Rice S. A. 1995; Identification of nuclear and nucleolar localization signals in herpes simplex virus regulatory proteins ICP27. Journal of Virology 69:935–947
    [Google Scholar]
  15. Puvion-Dutilleul F., Christensen M. E. 1993; Alterations of fibrillarin and nucleolar ultrastructure induced by adenovirus infection. European Journal of Cell Biology 61:168–176
    [Google Scholar]
  16. Rebelo L., Almeida F., Ramos C., Bohmann K., Lamond A. I., Carmo-Fonseca M. 1996; The dynamics of coiled bodies in the nucleus of adenovirus-infected cells. Molecular Biology of the Cell 7:1137–1151
    [Google Scholar]
  17. Russell W. C., Kemp G. D. 1995; The role of adenovirus structural proteins in the regulation of adenovirus infection. In The Molecular Repertoire of Adenoviruses 1 pp 81–98 Doerfler W., Bohm P. Edited by Heidelberg: Springer-Verlag;
    [Google Scholar]
  18. Stewart P. L., Fuller S. D., Burnett R. M. 1993; Difference imaging of adenovirus: bridging the resolution gap between X-ray crystallography and electron microscopy. EMBO Journal 12:2589–2599
    [Google Scholar]
  19. Tarassishin L. A., Russell W. C. 1997; Use of west-western blotting for detection of adenovirus receptors. Biochemistry Moscow: 6238–40
    [Google Scholar]
  20. Weber J. M. 1995; Adenovirus peptidase and its role in virus infection. In The Molecular Repertoire of Adenoviruses 1 pp 227–236 Doerfler W., Bohm P. Edited by Heidelberg: Springer-Verlag;
    [Google Scholar]
  21. Wickham T. J., Matthias P., Cheresti D. A., Nemerow G. R. 1993; Integrins αv β 3 and αv β 5 promote adenovirus internalisation but not virus attachment. Cell 73:309–319
    [Google Scholar]
  22. Winter N., d’Halluin J. 1991; Regulat ion of the biosynthesis of subgroup C adenovirus protein IVα2ʹ . Journal of Virology 10:118–194
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-7-1671
Loading
/content/journal/jgv/10.1099/0022-1317-79-7-1671
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error