1887

Abstract

Evidence of the functional significance of two naturally occurring mutations at nt 40 or 41 in the Sp1 motif in the promoter proximal segment of the upstream regulatory region (URR) of human papillomavirus (HPV) type 18 is presented. In electrophoretic mobility shift assays, Sp1 protein bound more efficiently to the Sp1 mutant motifs than to the prototype; while in both HeLa and HT3 cells, luciferase activity controlled by the mutant URRs was upregulated 2- and 3-fold, or 4- and 6fold, in comparison with the prototype URR or HeLa cell-derived URR respectively. The HeLa URR represents a more appropriate baseline for promoter activity, containing a series of point mutations representative of most HPV-18 cancer isolates, including one in the Yin Yang 1 (YY1) site at the P105 promoter. The effect of the Sp1 mutations was found to be largely maintained in the context of the HeLa URR containing the prototype YY1 site.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-7-1659
1998-07-01
2022-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/7/9680128.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-7-1659&mimeType=html&fmt=ahah

References

  1. Ausubel F. M., Brent R., Kingston R.E, Seidman J. G., Smith J. A., Struhl K. editors 1992 Biology, 2nd edn.. New York: Greene Publishing and Wiley-Interscience;
    [Google Scholar]
  2. Burger R. A., Monk B. J., Kurosaki T., Anton-Culver H. A., Vasilev S. A., Berman M. L., Wilczynski S. P. 1996; Human papillomavirus type 18 : association with poor prognosis in early stage cervical cancer. Journal of the National Cancer Institute 88:1361–1367
    [Google Scholar]
  3. Burnett A. F., Barnes W. A., Johnson J. C., Grenys M. D., Willett G. D., Barter J. F., Doniger J. 1992; Prognostic significance of polymerase chain reaction detected human papillomavirus of tumors and lymph nodes in surgically treated stage Ib cervical cancer. Gynecologic Oncology 47:343–347
    [Google Scholar]
  4. Chen T. M., Chen C. A., Wu C. C., Huang S. C., Chang C. F., Hsieh C. Y. 1994; The genotypes and prognostic significance of human papillomaviruses in cervical cancer. International Journal of Cancer 57:181–184
    [Google Scholar]
  5. Cole S. T., Danos O. 1987; The nucleotide sequence and genome organisation of human papillomavirus type 18. Journal of Molecular Biology 193:599–608
    [Google Scholar]
  6. Demeret C., Yaniv M., Thierry F. 1994; The E2 transcriptional repressor can compensate for Sp1 activation of the human papillomavirus type 18 early promoter. Journal of Virology 68:7075–7082
    [Google Scholar]
  7. Demeret C., Le Moal M., Yaniv M., Thierry F. 1995; Control of HPV 18 replication by cellular and viral transcription factors. Nucleic Acids Research 23:4777–4784
    [Google Scholar]
  8. de Wet J. R., Wood K. V., de Luca M., Helinski D. R., Subramani S. 1987; Firefly luciferase gene: Structure and expression in mammalian cells. Molecular and Cellular Biology 7:725–737
    [Google Scholar]
  9. Dong X.-P., Stubenrauch F., Beyer-Finkler E., Pfister H. 1994; Prevalence of deletions of YY1-binding sites in episomal HPV 16 DNA from cervical cancers. International Journal of Cancer 58:803–8081994
    [Google Scholar]
  10. Dyson M., Howley P. M., Munger K., Harlow E. 1989; The human papillomavirus 16 E 7 oncoprotein is able to bind to the retinoblastoma gene product. Science 243:934–937
    [Google Scholar]
  11. Hoppe-Seyler F., Butz K. 1992; Activation of human papillomavirus type 18 E6-E7 oncogene expression by transcription factor Sp1. Nucleic Acids Research 20:6701–6706
    [Google Scholar]
  12. Hoppe-Seyler F., Butz K. 1994; Cellular control of human papillomavirus oncogene transcription. Molecular Carcinogenesis 10:134–141
    [Google Scholar]
  13. Ip E. W. K., Collins R. J., Srivastava G., Cheung A. N., Wong L. C. 1992; Human papillomavirus and its prognostic significance in invasive carcinoma of the cervix in young patients. International Journal of Gynecological Pathology 11:258–265
    [Google Scholar]
  14. Kadonga J. T., Jones K. A., Tjian R. 1986; Promoter-specific activation of RNA polymerase II transcription by Spl . Trends in Biological Sciences 11:20–23
    [Google Scholar]
  15. Pfister H. 1996; The role of human papillomavirus in anogenital cancer. Obstetrics and Gynecology Clinics of North America 23:579–595
    [Google Scholar]
  16. Romanczuk H., Villa L. L., Schlegel R., Howley P. 1991; The viral transcriptional regulatory region upstream of the E6 and E7 genes is a major determinant of the differential immortalization activities of HPV types 16 and 18. Journal of Virology 65:2739–2744
    [Google Scholar]
  17. Rose B. R., Thompson C. H., Simpson J. M., Jarrett C. S., Elliott P. M., Tattersall M. H. N., Dalrymple C. P., Cossart Y. E. 1995; Human papillomavirus DNA as a prognostic indicator in early stage cervical cancer: a possible role for type 18. American Journal of Obstetrics and Gynecology 173:1461–1468
    [Google Scholar]
  18. Rose B. R., Thompson C. H., Zhang J., Stoeter M., Stephen A, Tattersall M. H. N., Cossart Y. E. 1997; Sequence variation in the upstream regulatory region of HPV 18 isolates from cervical cancers. Gynecologic Oncology 66:282–289
    [Google Scholar]
  19. Schwarz E., Schneider-Gadicke A., Roggenbuck B., Mayer W., Gissmann L., zur Hausen H. 1986; Expression of human papillomavirus DNA in cervical carcinoma cell lines. Banbury Reports 21:281–290
    [Google Scholar]
  20. Seto E., Lewis B., Shenk T. 1993; Interaction between transcription factors Sp1 and YY1. Nature 365:462–464
    [Google Scholar]
  21. Steger G., Corbach S. 1997; Dose dependent regulation of the early promoter of HPV 18 by the viral E2 protein. Journal of Virology 71:50–58
    [Google Scholar]
  22. Villa L. L., Schlegel R. 1991; Differences in transformation activity between HPV 18 and HPV 16 map to the viral LCR-E6-E7 region. Virology 181:374–377
    [Google Scholar]
  23. Walker J., Bloss J. D., Liao S.-Y., Berman M., Bergen S., Wilczynski S. P. 1989; Human papillomavirus genotype as a prognostic indicator in carcinoma ofthe uterine cervix. Obstetricsand Gynecology 74:781–785
    [Google Scholar]
  24. Werness B. A., Levine A. J., Howley P. M. 1990; Association of human papillomavirus types 16 and 18 proteins with p53. Science 248:76–79
    [Google Scholar]
  25. Workman J. L., Buchman A. R. 1993; Multiple functions of nucleosomes and regulatory factors in transcription. Trends in Biological Sciences 189:90–96
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-7-1659
Loading
/content/journal/jgv/10.1099/0022-1317-79-7-1659
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error