1887

Abstract

The recent discovery of a novel poxvirus [penguin-pox virus (PPV)] from Jackass penguins offers the potential of a unique candidate vaccine vector for use in mammals. Infectivity studies were therefore undertaken using a number of mammalian cell lines and chick embryo fibroblasts (CEF). It was shown that the simian CV-1 cell line was able to support replication of the PPV DNA, but no infectious progeny virus could be recovered from the infected cells. Electron microscopy was used to establish the extent of virus morphogenesis in CV-1 cells as compared to that in both chorio-allantoic membranes (CAMs) of hens’ eggs and CEF cells. It appears that CV-1 cells are able to support partial maturation of PPV, but that morphogenesis does not proceed to the stage of mature infectious particles. Vaccinia virus promoters were successful in achieving transient gene expression in PPV-infected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-7-1637
1998-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/7/9680125.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-7-1637&mimeType=html&fmt=ahah

References

  1. Arhelger R. B., Randall C. C. 1964; Electron microscopic observations on the development of fowlpox virus in chorioallantoic membrane. Virology 22:59–66
    [Google Scholar]
  2. Baxby D., Paoletti E. 1992; Potential use of non-replicating vectors as recombinant vaccines. Vaccine 10:8–9
    [Google Scholar]
  3. Binns M. M., Boursnell M. E. G., Tomley F. M., Campbell J. 1989; Analysis of the fowlpoxvirus gene encoding the 4b core polypeptide and demonstration that it possesses efficient promoter sequences. Virology 170:288–291
    [Google Scholar]
  4. Cadoz M., Strady A., Meinier B., Taylor J., Tartaglia J., Paoletti E., Plotkin S. 1992; Immunisation with canarypox virus expressing rabies glycoprotein. Lancet 339:1429–1432
    [Google Scholar]
  5. Cheville N. F. 1966; Cytopathic changes in fowlpox (turkey origin) inclusion body formation. American Journal of Pathology 49:723–736
    [Google Scholar]
  6. Cox W. I., Tartaglia J., Paoletti E. 1993; Induction of cytotoxic T lymphocytes by recombinant canarypox (ALVAC) and attenuated vaccinia (NYVAC) viruses expressing the HIV-1 envelope glycoprotein. Virology 195:845–850
    [Google Scholar]
  7. Fenner F., Burnet F. M. 1957; A short description of the poxvirus group (vaccinia and related viruses). Virology 4:305–314
    [Google Scholar]
  8. Guillaume J. C., Saiag P., Wechsler J., Lescs M. C., Roujeau J. C. 1991; Vaccinia from recombinant virus expressing HIV genes. Lancet 337:1034–1035
    [Google Scholar]
  9. Joklik W. K. 1962; The purification of four strains of poxvirus. Virology 18:9–18
    [Google Scholar]
  10. Kow D. 1992 Characterization of avipoxviruses for use in recombinant vaccines MSc thesis University of Cape Town, South Africa:
    [Google Scholar]
  11. MacGregor G. R., Caskey C. T. 1989; Construction of plasmids that express E. coli β-galactosidase in mammalian cells. Nucleic Acids Research 7:2365
    [Google Scholar]
  12. MacGregor G. R., Nolan G. P., Fiering S., Roederer M., Herzenberg L. A. 1991; Use of E. coli lacZ (β-galactosidase) as a reporter gene. Methods in Molecular Biology 7:217–325
    [Google Scholar]
  13. Moss B. 1990; Poxviridae and their replication. In Virology, 2nd edn.. pp 2079–2111 Fields B. N. others Edited by New York: Raven Press;
    [Google Scholar]
  14. Perkus M. E., Goebel S. J., Davis S. W., Johnson G. P., Limbach K., Norton E. K., Paoletti E. 1990; Vaccinia host range genes. Virology 179:276–286
    [Google Scholar]
  15. Purcell D. A., Clarke J. K., McFerran J. B., Hughes D. A. 1972; The morphogenesis of pigeonpox virus. Journal of General Virology 15:79–83
    [Google Scholar]
  16. Redfield R. R., Wright D. C., James W. D., Jones T. S., Brown C., Burke D. S. 1987; Disseminated vaccinia in a military recruit with human immunodeficiency virus (HIV) disease. New England Journal of Medicine 316:673–679
    [Google Scholar]
  17. Russell W. C., Newman C., Williamson D. H. 1975; A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature 253:461–462
    [Google Scholar]
  18. Sodeik B., Doms R. W., Ericsson M., Hiller G., Machamer C. E., van’t Hof W., van Meer G., Moss B., Griffiths G. 1993; Assembly of vaccinia virus: role of the intermediate compartment between the endoplasmic compartment and the Golgi stacks. Journal of Cell Biology 121:521–541
    [Google Scholar]
  19. Somogyi P., Frazier J., Skinner M. A. 1993; Fowlpox virus host range restriction: gene expression, DNA replication and morphogenesis in nonpermissive mammalian cells. Virology 197:439–444
    [Google Scholar]
  20. Spehner D., Gillard S., Drillien R., Kirn A. 1988; A cowpox virus gene required for multiplication in Chinese hamster ovary cells. Journal of Virology 62:1297–1304
    [Google Scholar]
  21. Stern W., Dales S. 1976; Biogenesis of vaccinia: relationship of the envelope to virus assembly. Virology 75:242–255
    [Google Scholar]
  22. Sutter G., Moss B. 1992; Nonreplicating vaccinia vector efficiently expresses recombinant genes. Proceedings of the National Academy of Sciences, USA 89:10847–10851
    [Google Scholar]
  23. Tartaglia J., Jarrett O., Neil J. C., Desmettre P., Paoletti E. 1993; Protection of cats against feline leukemia virus by vaccination with a canarypox virus recombinant, ALVAC-FL. Journal of Virology 67:2370–2375
    [Google Scholar]
  24. Taylor J., Weinberg R., Languet B., Desmettre P., Paoletti E. 1988; Recombinant fowlpox virus inducing protective immunity in non-avian species. Vaccine 6:497–503
    [Google Scholar]
  25. Taylor J., Trimarchi C., Weinberg R., Languet B., Guillemin F., Desmettre P., Paoletti E. 1991; Efficacy studies on a canarypox- rabies recombinant virus. Vaccine 9:190–193
    [Google Scholar]
  26. Taylor J., Weinberg R., Tartaglia J., Richardson C., Alkhatib G., Briedis D., Appel M., Norton E., Paoletti E. 1992; Nonreplicating viral vectors as potential vaccines: recombinant canarypox virus expressing measles virus fusion (F) and hemagglutinin (HA) glycoproteins. Virology 187:321–328
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-7-1637
Loading
/content/journal/jgv/10.1099/0022-1317-79-7-1637
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error