1887

Abstract

The region upstream of the multicapsid nucleopolyhedrovirus (SeMNPV) ubi-quitin gene contains four near-identical 68-bp-long palindromic repeats. This region, named Se6 and located at map unit (m.u.) 88 of the SeMNPV genome on pSeRI-2.2, showed structural homology to previously identified homologous regions (s) in a number of other baculoviruses. s function as enhancers of transcription and as putative origins () of baculovirus DNA replication. Five additional s (Se1-Se5) were identified on the SeMNPV genome by Southern blot hybridization with an 18-bp-long oligonucleotide complementary to a sequence conserved within the arms of the four palindromic repeats of Se6. Se1-Se6 were dispersed on the SeMNPV genome at m.u. 8·0, 30·0, 38·5, 51·0, 77·0 and 88·0, respectively. Sequence analysis of these s confirmed the presence of palindromic repeats, highly similar to those found in pSeRI-2.2. The number of palindromes varied from one (Se4) to nine (Se1) per . The Ses are all present in non-coding regions of the SeMNPV genome and also contain multiple putative transcription recognition sequences. Plasmids containing either of the Ses replicated in an SeMNPV-dependent DNA replication assay. The Ses were unable to replicate in an AcMNPV-dependent DNA replication assay. This was in contrast to the previously observed SeMNPV non- type , which replicated in the presence of both AcMNPV and SeMNPV. These data suggest that the replication of SeMNPV and the role of s in this process is highly specific.

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1998-06-01
2024-04-25
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