RT Journal Article SR Electronic(1) A1 Bringloe, D. H. A1 Gultyaev, A. P. A1 Pelpel, M. A1 Pleij, C. W. A. A1 Coutts, R. H. A.YR 1998 T1 The nucleotide sequence of satellite tobacco necrosis virus strain C and helper-assisted replication of wild-type and mutant clones of the virus. JF Journal of General Virology, VO 79 IS 6 SP 1539 OP 1546 DO https://doi.org/10.1099/0022-1317-79-6-1539 PB Microbiology Society, SN 1465-2099, AB The complete nucleotide sequence of satellite tobacco necrosis virus strain C (STNV-C) was determined. The genome has a similar overall organization to two STNV isolates studied previously but differs significantly from them in the secondary structure of the translated and untranslated regions (UTRs). STNV-C RNA is naturally uncapped and contains 1221 nt: 101 nt in the 5′ UTR, 606 nt in the capsid protein (CP) coding region and 514 nt in the 3′ UTR. Using the known sequences of STNV-C and tobacco necrosis virus strain D (TNV-D) RNAs, full-length cDNA clones of both RNAs were constructed. Synthetic transcripts derived from STNV-C cDNA clones only replicated in plants and protoplasts when co-inoculated with TNV-D transcripts. A number of mutant clones in both the 3′ and the 5′ STNV-C RNA UTRs were constructed which disrupted putative cis-acting elements recognized by helper virus polymerase. Deletion analysis revealed an essential requirement of all 3′ and 5′ proximal sequences in the STNV-C UTRs for replication. However, an internal region in the 3′ UTR could be deleted without loss of infectivity. Likewise, the entire STNV-C CP-encoding region could be deleted and replaced with a marker gene of a similar size without loss of transcript accumulation in plants., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-6-1539