1887

Abstract

The complete nucleotide sequence of satellite tobacco necrosis virus strain C (STNV-C) was determined. The genome has a similar overall organization to two STNV isolates studied previously but differs significantly from them in the secondary structure of the translated and untranslated regions (UTRs). STNV-C RNA is naturally uncapped and contains 1221 nt: 101 nt in the 5′ UTR, 606 nt in the capsid protein (CP) coding region and 514 nt in the 3′ UTR. Using the known sequences of STNV-C and tobacco necrosis virus strain D (TNV-D) RNAs, full-length cDNA clones of both RNAs were constructed. Synthetic transcripts derived from STNV-C cDNA clones only replicated in plants and protoplasts when co-inoculated with TNV-D transcripts. A number of mutant clones in both the 3′ and the 5′ STNV-C RNA UTRs were constructed which disrupted putative -acting elements recognized by helper virus polymerase. Deletion analysis revealed an essential requirement of all 3′ and 5′ proximal sequences in the STNV-C UTRs for replication. However, an internal region in the 3′ UTR could be deleted without loss of infectivity. Likewise, the entire STNV-C CP-encoding region could be deleted and replaced with a marker gene of a similar size without loss of transcript accumulation in plants.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-6-1539
1998-06-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/6/9634099.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-6-1539&mimeType=html&fmt=ahah

References

  1. Ameloot P. 1990 Funktionele analyse van het tabaksnekrose satellietvirus genoom PhD thesis University of Ghent, Belgium:
    [Google Scholar]
  2. Andriessen M., Meulewaeter F., Cornelissen M. 1995; Expression of tobacco necrosis virus open reading frames 1 and 2 is sufficient for the replication of satellite tobacco necrosis virus. Virology 212:222–224
    [Google Scholar]
  3. Buck K. W. 1996; Comparison of the replication of positive-stranded RNA viruses of plants and animals. Advances in Virus Research 47:159–251
    [Google Scholar]
  4. Chen E. Y., Seeberg P. H. 1985; Supercoil sequencing: a fast and reliable method for sequencing plasmid DNA. DNA 4:165–170
    [Google Scholar]
  5. Coutts R. H. A., Rigden J. E., Slabas A. E., Lomonossoff G. P., Wise P. J. 1991; The complete nucleotide sequence of tobacco necrosis virus strain D. Journal of General Virology 72:1521–1529
    [Google Scholar]
  6. Danthinne X., Seurinck J., Van Montagu M., Pleij C. W. A., Van Emmelo J. 1991; Structural similarities between the RNAs of two satellites of tobacco necrosis virus. Virology 185:605–614
    [Google Scholar]
  7. Danthinne X., Seurinck J., Meulewaeter F., Van Montagu M., Cornelissen M. 1993; The 3′ untranslated region of satellite tobacco necrosis virus RNA stimulates translation in vitro . Molecular and Cellular Biology 13:3340–3349
    [Google Scholar]
  8. Dean C., Van Den Elzen P., Tamaki S., Dunsmuir P., Bedbrook J. 1985; Differential expression of the eight genes of the petunia ribulose biphosphate carboxylase multigene family. EMBO Journal 4:3055–3061
    [Google Scholar]
  9. Devereux J., Haerberli P., Smithies G. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  10. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  11. Gultyaev A. P., Van Batenburg F. H. D., Pleij C. W. A. 1995; The computer simulation of RNA folding pathways using a genetic algorithm. Journal of Molecular Biology 250:37–51
    [Google Scholar]
  12. Havelda Z., Burgyan J. 1995; 3′ terminal putative stem-loop structure required for the accumulation of cymbidium ringspot viral RNA. Virology 214:269–272
    [Google Scholar]
  13. Havelda Z., Dalmay T., Burgyan J. 1995; Localization of cis-acting sequences essential for cymbidium ringspot defective interfering RNA replication. Journal of General Virology 76:2311–2316
    [Google Scholar]
  14. Horst J., Fraenkel-Conrat H., Mandeles S. 1971; Terminal heterogeneity at both ends of the satellite tobacco necrosis virus ribonucleic acid. Biochemistry 10:4748–4752
    [Google Scholar]
  15. Kassanis B., Phillips M. P. 1970; Serological relationships of strains of tobacco necrosis virus and their ability to activate strains of satellite virus. Journal of General Virology 9:119–126
    [Google Scholar]
  16. Kurath G., Palukaitis P. 1987; Biological activity of T7 transcripts of a prototype clone and a sequence variant clone of a satellite RNA of cucumber mosaic virus. Virology 159:199–208
    [Google Scholar]
  17. Meulewaeter F., Seurinck J., Van Emmelo J. 1990; Genome structure of tobacco necrosis virus strain A. Virology 177:699–709
    [Google Scholar]
  18. Meulewaeter F., Danthinne X., Coutts R. H. A., Van Emmelo J. 1993; Specificity of satellite activation by tobacco necrosis virus correlates with nucleic acid hybridisation pattern between helper virus isolates. Virology 193:971–973
    [Google Scholar]
  19. Mossop D. W., Francki R. I. B. 1979; The stability of satellite viral RNAs in vivo and in vitro . Virology 94:243–253
    [Google Scholar]
  20. Offei S. K., Coutts R. H. A. 1996; Location of the 5′ termini of tobacco necrosis virus strain D subgenomic mRNAs. Journal of Phytopathology 144:13–17
    [Google Scholar]
  21. Routh G., Yassi M. N. A., Rao A. L. N., Mirkov T. E., Dodds J. A. 1997; Replication of wild-type and mutant clones of satellite tobacco mosaic virus in Nicotiana benthamiana protoplasts. Journal of General Virology 78:1271–1275
    [Google Scholar]
  22. Russo M., Burgyan J., Martelli G. P. 1994; The molecular biology of Tombusviridae . Advances in Virus Research 44:321–428
    [Google Scholar]
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Song C., Simon A. E. 1995; Requirement of a 3′-terminal stem-loop in in vitro transcription by an RNA-dependent RNA polymerase. Journal of Molecular Biology 254:6–14
    [Google Scholar]
  25. Uyemoto J. K. 1981; Tobacco necrosis and satellite viruses. In Handbook of Plant Virus Infections and Comparative Diagnosis pp. 123–145 Kurstak E. Edited by North Holland: Elsevier Biomedical Press;
    [Google Scholar]
  26. Van Emmelo J., Ameloot P., Fiers W. 1987; Expression in plants of the cloned satellite tobacco necrosis genome and of derived insertion mutants. Virology 157:480–487
    [Google Scholar]
  27. Ysebaert M., Van Emmelo J., Fiers W. 1980; Total nucleotide sequence of a nearly full-size DNA copy of satellite tobacco necrosis virus RNA. Journal of Molecular Biology 143:273–287
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-6-1539
Loading
/content/journal/jgv/10.1099/0022-1317-79-6-1539
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error