1887

Abstract

The proteins encoded by rice black streaked dwarf fijivirus (RBSDV) genomic segments 7–10 (S7-S10) were characterized. Open reading frames (ORFs) from these segments were expressed as fusion proteins in . Antibodies raised against the expressed products were used as probes to determine whether the viral ORFs encode structural proteins. In Western blots, antibodies to the expressed S8 and S10 products reacted with a core capsid (65 kDa) and a major outer capsid (56 kDa) protein, respectively, while none of the antibodies to S7 and S9 products reacted with structural proteins. Antisera to RBSDV S7 ORF1 and S9 ORF1 each detected a single protein of the predicted size in total protein extracts from infected rice plants and viruliferous . Immuno- electron microscopy revealed that antibodies to RBSDV S7 ORF1 and RBSDV S9 ORF1 reacted with tubular structures and viroplasm, respectively, in sections of both infected maize plants and viruliferous . Antisera to ORF2 of S7 and S9 failed to detect any proteins in the infected tissue using either Western blotting or immuno-electron microscopic techniques.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-6-1487
1998-06-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/6/9634092.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-6-1487&mimeType=html&fmt=ahah

References

  1. Angenon G., Votile J., Kurkela S. A., Teeri T. H., Botterman J., Van Montagu M., Depicker A. 1989; Expression of dicistronic transcriptional units in transgenic tobacco. Molecular & Cellular Biology 9:5676–5684
    [Google Scholar]
  2. Azuhata F., Uyeda I., Shikata E. 1992; Conserved terminal nucleotide sequences in the genome of rice black streaked dwarf virus. Journal of General Virology 73:1593–1595
    [Google Scholar]
  3. Azuhata F., Uyeda I., Kimura I., Shikata E. 1993; Close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses. Journal of General Virology 74:1227–1232
    [Google Scholar]
  4. Blake M. S., Johnston K. H., Russell-Jones G. J., Gotschlich E. C. 1984; A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western-blots. Analytical Biochemistry 136:175–179
    [Google Scholar]
  5. Bradford M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 72:248
    [Google Scholar]
  6. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  7. Deom C. M., Schubert K. R., Wolf S., Holt C. A., Lucas W. J., Beachy R. N. 1990; Molecular characterisation and biological function of the movement protein of tobacco mosaic virus in transgenic plants. Proceedings of the National Academy of Sciences, USA 87:3284–3288
    [Google Scholar]
  8. Diaz-Ruiz J. R., Kaper J. M. 1978; Isolation of viral double stranded RNAs using a LiCl fractionation procedure. Preparative Biochemistry 80:1–17
    [Google Scholar]
  9. Gerola F. M., Bassi M. 1966; An electron microscopy study of leaf vein tumours from maize plant experimentally infected with maize rough dwarf virus. Caryologia 19:13–40
    [Google Scholar]
  10. Isogai M., Azuhata F., Uyeda I., Shikata E., Kimura I. 1995; Genomic relationships between rice black streaked dwarf and maize rough dwarf fijiviruses detected by nucleic acid hybridization. Annals of the Phytopathological Society of Japan 61:513–518
    [Google Scholar]
  11. Isogai M., Uyeda I., Lindsten K. 1998; Taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome. Journal of General Virology 79:1479–1485
    [Google Scholar]
  12. Jang S. K., Krausslich H.-G., Nicklin M., Duke G., Palmenberg A. C., Wimmer E. 1988; A segment of the 5′ nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes. Journal of Virology 62:2636–2643
    [Google Scholar]
  13. Kaufman R., Murtha P., Davies M. 1987; Translational efficiency of polycistronic mRNAs and their utilisation to express heterologous genes in mammalian cell. EMBO Journal 6:187–193
    [Google Scholar]
  14. Kozak M. 1987; Effects of intercistronic length on the efficiency of reinitiation by eukaryotic ribosomes. Molecular & Cellular Biology 7:3438–3445
    [Google Scholar]
  15. Kuribayashi K., Shinkai A. 1952; On the rice black-streaked dwarf disease, a new disease of rice in Japan. Annals of the Phytopathological Society of Japan 16:41
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  17. Masuta C., Furuno M., Tanaka H., Yamada M., Koiwai A. 1992; Molecular cloning of a cDNA clone for tobacco lipid transfer protein and expression of the functional protein in Escherichia coli . FEBS Letters 311:119–123
    [Google Scholar]
  18. Marzachí C. M., Boccardo G., Nuss D. 1991; Cloning of the maize rough dwarf virus genome: molecular confirmation of the plant-reovirus classification scheme and identification of two large nonoverlapping coding domains with a single genomic segment. Virology 180:518–526
    [Google Scholar]
  19. Marzachí C. M., d’Aquilio S. A. M., Boccardo G. 1996; The double-stranded RNA genome of maize rough dwarf fijivirus contains both mono and dicistronic segments. European Journal of Plant Pathology 102:601–605
    [Google Scholar]
  20. Milne R. G. 1980; Electron microscopy of thin sections of Italian ryegrass infected with both ryegrass cryptic virus and oat sterile dwarf virus. Microbiologica 3:333–341
    [Google Scholar]
  21. Milne R. G., Conti M., Lisa V. 1973; Partial purification, structure and infectivity of complete maize rough dwarf virus particles. Virology 53:130–141
    [Google Scholar]
  22. Nakashima N., Koizumi M., Watanabe H., Noda H. 1996; Complete nucleotide sequence of the Nilaparvata lugens reovirus : a putative member of the genus Fijivirus . Journal of General Virology 77:139–146
    [Google Scholar]
  23. Pelletier J., Sonenberg N. 1988; Internal initiation oftranslation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature 334:320–325
    [Google Scholar]
  24. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  26. Shikata E. 1969; Electron microscopic studies on rice viruses. In IRRI : The Virus Disease of the Rice Plant pp. 223–240 Baltimore: Johns Hopkins University Press;
    [Google Scholar]
  27. Shikata E., Kitagawa Y. 1977; Rice black-streaked dwarf virus : its properties, morphology and intracellular localisation. Virology 77:826
    [Google Scholar]
  28. Shinkai A. 1962; Studies on insect transmission of rice virus disease. Bulletin of the National Institute of Agricultural Sciences (Japan) c14:1–112
    [Google Scholar]
  29. Storms M., Kormelink R., Peters D., van Lent J., Goldbach R. 1995; The nonstructure NSm protein of tomato spotted wilt virus induces tubular structure in plant and insect cells. Virology 214:485–493
    [Google Scholar]
  30. Suzuki Y., Toriyama S., Matsuda I., Kojima M. 1993; Detection of a picorna-like virus, himetobi P virus, in organs and tissues of Laodelphax striatellus by immunogold labelling and enzyme-linked immunosorbent assay. Journal of Invertebrate Pathology 62:99–104
    [Google Scholar]
  31. Uyeda I., Azuhata F., Shikata E. 1990; Nucleotide sequence of rice black-streaked dwarf virus genome segment 10. Proceedings of the Japan Academy 66:37–40
    [Google Scholar]
  32. van Lent J., Wellink J., Goldbach R. 1990; Evidence for the involvement of the 58 k and 48 k proteins in the intercellular movement of cowpea mosaic virus. Journal of General Virology 71:219–223
    [Google Scholar]
  33. Vidano C. 1970; Phases of maize rough dwarf virus multiplication in the vector Laodelphax striatellus (FALLÉN). Virology 41:218–232
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-6-1487
Loading
/content/journal/jgv/10.1099/0022-1317-79-6-1487
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error