1887

Abstract

A body cavity lymphoma-derived cell line (BC1), known to carry both Epstein-Barr virus (EBV) and human herpes virus type 8 (HHV-8; or Kaposi’s sarcoma-associated herpesvirus, KSHV), was analysed for the expression of EBV-encoded, growth transformation-associated antigens and cellular phenotype by immunofluorescence staining, Western blotting, RT-PCR and flow cytometry. A similar phenotypic analysis was also performed on another body cavity lymphoma line, BCBL1, that is singly infected with HHV-8. Phenotypically, the two lines were closely similar. Although both lines are known to carry rearranged immunoglobulin genes, they were mostly negative for B-cell surface markers. Both expressed the HHV-8-encoded nuclear antigen (LNA1). Similarly to Epstein-Barr nuclear antigen type 1 (EBNA1), LNA1 was associated with the chromatin in interphase nuclei and the mitotic chromosomes in metaphase. It accumulated in a few well-circumscribed nuclear bodies that did not colocalize with EBNA1. BC1 cells expressed EBNA1, LMP2A and EBV-encoded small RNAs but not EBNA2-6,LMP1 and LMP2B. They were thus similar to type I Burkitt ’s lymphoma cells and latently infected peripheral B-cells. Analysis of the splicing pattern of the EBNA1-encoding message by RT-PCR showed that BC1 cells used theQUK but not the YUK splice, indicating that the mRNA was initiated from Qp and not from Cp or Wp.

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1998-06-01
2022-05-23
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References

  1. Altiok E., Klein G., Zech L., Uno M., Henriksson B. E., Battat S., Ono Y., Ernberg I. 1989; Epstein-Barr virus-transformed pro-B cells are prone to illegitimate recombination between the switch region of the mu chain gene and other chromosomes. Proceedings of the National Academy of Sciences, USA 86:6333–6337
    [Google Scholar]
  2. Altiok E., Minarovits J., Hu L. F., Contreras-Brodin B., Klein G., Ernberg I. 1992; Host-cell-phenotype-dependent control of the BCR2/BWR1 promoter complex regulates the expression of Epstein-Barr virus nuclear antigens 2-6. Proceedings of the National Academy of Sciences, USA 89:905–909
    [Google Scholar]
  3. Arvanitakis L., Mesri E. A., Nador R. G., Said J. W., Asch A. S., Knowles D. M., Cesarman E. 1996; Establishment and characterization of a primary effusion (body cavity-based) lymphoma cell line (BC-3) harboring Kaposi’s sarcoma-associated herpesvirus (KSHV/HHV-8) in the absence of Epstein-Barr virus. Blood 88:2648–2654
    [Google Scholar]
  4. Bodescot M., Perricaudet M. 1986; Epstein-Barr virus mRNAs produced by alternative splicing. Nucleic Acids Research 14:7103–7114
    [Google Scholar]
  5. Bodescot M., Perricaudet M., Farrell P. J. 1987; A promoter for the highly spliced EBNA family of RNAs of Epstein-Barr virus. Journal of Virology 61:3424–3430
    [Google Scholar]
  6. Carbone A., Gloghini A., Vaccher E., Zagonel V., Pastore C., Dallapalma P., Branz F., Saglio G., Volpe R., Tirelli U., Gaidano G. 1996; Kaposi’s sarcoma-associated herpesvirus DNA sequences in AIDS-related and AIDS-unrelated lymphomatous effusions. British Journal of Haematology 94:533–543
    [Google Scholar]
  7. Cesarman E., Chang Y., Moore P. S., Said J. W., Knowles D. M. 1995a; Kaposi’s sarcoma-associated herpesvirus-like DNA sequences in AIDS-related body-cavity-based lymphomas. New England Journal of Medicine 332:1186–1191
    [Google Scholar]
  8. Cesarman E., Moore P. S., Rao P. H., Inghirami G., Knowles D. M., Chang Y. 1995b; In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi’s sarcoma-associated herpesvirus-like (KSHV) DNA sequences. Blood 86:2708–2714
    [Google Scholar]
  9. Cesarman E., Nador R. G., Aozasa K., Delsol G., Said J. W., Knowles D. M. 1996; Kaposi’s sarcoma-associated herpesvirus in non-AIDS-related lymphomas occurring in body cavities. American Journal of Pathology 149:53–57
    [Google Scholar]
  10. Chang Y., Moore P. S. 1996; Kaposi’s sarcoma (KS)-associated herpesvirus and its role in KS. Infectious Agents & Disease 5:215–222
    [Google Scholar]
  11. Chen F., Zou J. Z., di Renzo L., Winberg G., Hu L. F., Klein E., Klein G., Ernberg I. 1995; A subpopulation of normal B cells latently infected with Epstein-Barr virus resembles Burkitt lymphoma cells in expressing EBNA-1 but not EBNA-2 or LMP1. Journal of Virology 69:3752–3758
    [Google Scholar]
  12. Contreras-Brodin B. A., Anvret M., Imreh S., Altiok E., Klein G., Masucci M. G. 1991; B cell phenotype-dependent expression of the Epstein-Barr virus nuclear antigens EBNA-2 to EBNA-6: studies with somatic cell hybrids. Journal of General Virology 72:3025–3033
    [Google Scholar]
  13. Contreras-Salazar B., Klein G., Masucci M. G. 1989; Host cell-dependent regulation of growth transformation-associated Epstein-Barr virus antigens in somatic cell hybrids. Journal of Virology 63:2768–2772
    [Google Scholar]
  14. Ehlin-Henriksson B., Manneborg-Sandlund A., Klein G. 1987; Expression of B-cell specific markers in different Burkitt lymphoma subgroups. International Journal of Cancer 39:211–218
    [Google Scholar]
  15. Fahraeus R., Fu H. L., Ernberg I., Finke J., Rowe M., Klein G., Falk K., Nilsson E., Yadav M., Busson P. others 1988; Expression of Epstein-Barr virus-encoded proteins in nasopharyngeal carcinoma. International Journal of Cancer 42:329–338
    [Google Scholar]
  16. Fahraeus R., Jansson A., Sjoblom A., Nilsson T., Klein G., Rymo L. 1993; Cell phenotype-dependent control of Epstein-Barr virus latent membrane protein 1 gene regulatory sequences. Virology 195:71–80
    [Google Scholar]
  17. Gao S. J., Kingsley L., Hoover D. R., Spira T. J., Rinaldo C. R., Saah A., Phair J., Detels R., Parry P., Chang Y., Moore P. S. 1996a; Seroconversion to antibodies against Kaposi’s sarcoma-associated herpes-virus-related latent nuclear antigens before the development of Kaposi’s sarcoma. New England Journal of Medicine 335:233–241
    [Google Scholar]
  18. Gao S. J., Kingsley L., Li M., Zheng W., Parravicini C., Ziegler J., Newton R., Rinaldo C. R., Saah A., Phair J., Detels R., Chang Y. A., Moore P. S. 1996b; KSHV antibodies among Americans, Italians and Ugandans with and without Kaposi’s sarcoma. Nature Medicine 2:925–928
    [Google Scholar]
  19. Gessain A., Sudaka A., Briere J., Fouchard N., Nicola M. A., Rio B., Arborio M., Troussard X., Audouin J., Diebold J., de Thé G. 1996; Kaposi sarcoma-associated herpes-like virus (human herpesvirus type 8) DNA sequences in multicentric Castleman’s disease : is there any relevant association in non-human immunodeficiency virus-infected patients?. Blood 87:414–416
    [Google Scholar]
  20. Hitt M. M., Allday M. J., Hara T., Karran L., Jones M. D., Busson P., Tursz T., Ernberg I., Griffin B. E. 1989; EBV gene expression in an NPC-related tumour. EMBO Journal 8:2639–2651
    [Google Scholar]
  21. Horenstein M. G., Nador R. G., Chadburn A., Hyjek E. M., Inghirami G., Knowles D. M., Cesarman E. 1997; Epstein-Barr virus latent gene expression in primary effusion lymphomas containing Kaposi’s sarcoma-associated herpesvirus/human herpesvirus-8. Blood 90:1186–1191
    [Google Scholar]
  22. Jiang W. Q., Wendel-Hansen V., Lundkvist A., Ringertz N., Klein G., Rosen A. 1991; Intranuclear distribution of Epstein-Barr virus- encoded nuclear antigens EBNA-1, -2, -3 and -5. Journal of Cell Science 99:497–502
    [Google Scholar]
  23. Kedes D. H., Operskalski E., Busch M., Kohn R., Flood J., Ganem D. 1996; The seroepidemiology of human herpesvirus 8 (Kaposi’s sarcoma-associated herpesvirus) - distribution of infection in KS risk groups and evidence for sexual transmission. Nature Medicine 2:918–924
    [Google Scholar]
  24. Lewin N., Avila-Carino J., Minarovits J., Lennette E., Brautbar C., Mellstedt H., Klein G., Klein E. 1995; Detection of two Epstein-Barr-virus (EBV)-carrying leukemic cell clones in a patient with chronic lymphocytic leukemia (CLL). International Journal of Cancer 61:159–164
    [Google Scholar]
  25. Miles S. A. 1996; Pathog enesis of AIDS-related Kaposi’s sarcoma - evidence of a viral etiology. Hematology/Oncology Clinics of North America 10:1011–1021
    [Google Scholar]
  26. Nador R. G., Cesarman E., Chadburn A., Dawson D. B., Ansari M. Q., Said J., Knowles D. M. 1996; Primary effusion lymphoma - a distinct clinicopathologic entity associated with the Kaposi’s sarcoma- associated herpes virus. Blood 88:645–656
    [Google Scholar]
  27. Pallesen G., Hamilton-Dutoit S. J., Rowe M., Young L. S. 1991; Expression of Epstein-Barr virus latent gene products in tumour cells of Hodgkin’s disease. Lancet 337:320–322
    [Google Scholar]
  28. Pokrovskaja K., Ehlin-Henriksson B., Bartkova J., Bartek J., Scuderi R., Szekely L., Wiman K. G., Klein G. 1996; Phenotype related differences in the expression of D-type cyclins in human B cell-derived lines. Cell Growth and Differentiation 7:1723–1732
    [Google Scholar]
  29. Rainbow L., Platt G. M., Simpson G. R., Sarid R., Gao S.-J., Stoiber C., Herrington S., Moore P. S., Schulz T. F. 1997; The 222- to 234-kilodalton latent nuclear protein (LNA) of Kaposi’s sarcoma-associated herpesvirus (Human herpesvirus 8) is encoded by orf73 and is a component of the latency-associated nuclear antigen. Journal of Virology 71:5915–5921
    [Google Scholar]
  30. Renne R., Zhong W., Herndier B., McGrath M., Abbey N., Kedes D., Ganem D. 1996; Lytic growth of Kaposi’s sarcoma-associated herpesvirus (human herpesvirus 8) in culture. Nature Medicine 2:342–346
    [Google Scholar]
  31. Rettig M. B., Ma H. J., Vescio R. A., Pold M., Schiller G., Belson D., Savage A., Nishikubo C., Wu C., Fraser J., Said J. W., Berenson J. R. 1997; Kaposi’s-sarcoma-associated herpesvirus infection of bone marrow dendritic cells from multiple myeloma patients. Science 276:1851–1854
    [Google Scholar]
  32. Rowe D. T., Rowe M., Evan G. I., Wallace L. E., Farrell P. J., Rickinson A. B. 1986; Restricted expression of EBV latent genes and T-lymphocyte-detected membrane antigen in Burkitt’s lymphoma cells. EMBO Journal 5:2599–2607
    [Google Scholar]
  33. Rowe M., Rowe D. T., Gregory C. D., Young L. S., Farrell P. J., Rupani H., Rickinson A. B. 1987; Differences in B cell growth phenotype reflect novel patterns of Epstein-Barr virus latent gene expression in Burkitt’s lymphoma cells. EMBO Journal 6:2743–2751
    [Google Scholar]
  34. Said J. W., Tasaka T., Takeuchi S., Asou H., Devos S., Cesarman E., Knowles D. M., Koeffler H. P. 1996; Primary effusion lymphoma in women - report of two cases of Kaposi’s sarcoma herpes virus-associated effusion-based lymphoma in human immunodeficiency virus-negative women. Blood 88:3124–3128
    [Google Scholar]
  35. Schaefer B. C., Strominger J. L., Speck S. H. 1995; Redefining the Epstein-Barr virus-encoded nuclear antigen EBNA-1 gene promoter and transcription initiation site in group I Burkitt lymphoma cell lines. Proceedings of the National Academy of Sciences, USA 92:10565–10569
    [Google Scholar]
  36. Seabright M. 1971; A rapid banding technique for human chromosomes. Lancet ii:971–972
    [Google Scholar]
  37. Simpson G. R., Schulz T. F., Whitby D., Cook P. M., Boshoff C., Rainbow L., Howard M. R., Gao S. J., Bohenzky R. A., Simmonds P., Lee C., Deruiter A., Hatzakis A., Tedder R. S., Weller I. V. D., Weiss R. A., Moore P. S. 1996; Prevalence of Kaposi’s sarcoma associated herpesvirus infection measured by antibodies to recombinant capsid protein and latent immunofluorescence antigen. Lancet 348:1133–1138
    [Google Scholar]
  38. Szekely L., Uzvolgyi E., Jiang W. Q., Durko M., Wiman K. G., Klein G., Sumegi J. 1991; Subcellular localization of the retinoblastoma protein. Cell Growth & Differentiation 2:287–295
    [Google Scholar]
  39. Szekely L., Selivanova G., Magnusson K. P., Klein G., Wiman K. G. 1993; EBNA-5, an Epstein-Barr virus-encoded nuclear antigen, binds to the retinoblastoma and p53 proteins. Proceedings of the National Academy of Sciences, USA 90:5455–5459
    [Google Scholar]
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