1887

Abstract

Cytoplasmic extracts prepared from avian reovirus (ARV) strain S1133-infected chicken embryo fibroblasts were examined for the presence of RNA- binding proteins in order to identify and characterize ARV RNA-binding proteins. Analysis of binding activity to poly(A)-Sepharose indicated that infected cells contained significant amounts of a protein that co-migrated with ARV protein NS present in total virus-infected cell extracts. Determination of the N-terminal amino acid sequence of several peptide fragments generated by V8 protease digestion of the poly(A)-Sepharose- purified protein confirmed that this viral protein was NS. Competition assays showed that singlestranded RNA from the unrelated avian pathogen infectious bursal disease virus was able to compete for binding of NS to poly(A)-Sepharose. These data suggest that ARV NS binds to single-stranded RNA in a nucleotide sequence non-specific manner and is functionally similar to its counterpart specified by mammalian reovirus.

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/content/journal/jgv/10.1099/0022-1317-79-6-1411
1998-06-01
2021-10-16
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References

  1. Annadata M., Vakharia V. N. 1994; Comparative analysis of virus- induced polypeptides of an avirulent and a virulent strain of avian reovirus. Avian Diseases 38:244–250
    [Google Scholar]
  2. Antczak J. B., Joklik W. K. 1992; Reovirus genome segment assortment into progeny genomes studied by the use of monoclonal antibodies directed against reovirus proteins. Virology 187:760–776
    [Google Scholar]
  3. Chiu C. J., Lee L. H. 1997; Cloning and nucleotide sequencing of the S4 genome segment of avian reovirus S1133. Archives of Virology 142:2515–2520
    [Google Scholar]
  4. Cleveland D. W., Fischer S. G., Kirschner M. C., Laemmli U. K. 1977; Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry 252:1102–1106
    [Google Scholar]
  5. Gouvea V. S., Schnitzer T. J. 1982; Polymorphism of the genomic RNAs among the avian reoviruses. Journal of General Virology 61:87–91
    [Google Scholar]
  6. Huismans H., Joklik W. K. 1976; Reovirus-coded polypeptides in infected cells: isolation of two native monomeric polypeptides with affinity for single-stranded and double-stranded RNA, respectively. Virology 70:411–424
    [Google Scholar]
  7. Kattoura M. D., Clapp L. L., Patton J. T. 1992; The rotavirus nonstructural protein, NS35, possesses RNA-binding activity in vitro and in vivo . Virology 191:698–708
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  9. Lee L. H. 1992; Characterization of nonradioactive hybridization probes for detecting infectious bursal disease virus. Journal of Virological Methods 38:81–92
    [Google Scholar]
  10. Matsudaira P. 1987; Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. Journal of Biological Chemistry 262:10035–10038
    [Google Scholar]
  11. Patton J. T. 1995; Structure and function of the rotavirus RNA-binding proteins. Journal of General Virology 76:2633–2644
    [Google Scholar]
  12. Varela R., Benavente J. 1994; Protein coding assignment of avian reovirus strain S1133. Journal of Virology 68:6775–6777
    [Google Scholar]
  13. Wickramasinghe R., Meanger J., Enriguez C. E., Wilcox G. E. 1993; Avian reovirus proteins associated with neutralization of virus infectivity. Virology 194:688–696
    [Google Scholar]
  14. Wu W. Y., Shien J. H., Lee L. H., Shick H. K. 1994; Analysis of the double-stranded RNA genome segments of avian reovirus field isolates. Journal of Virological Methods 48:119–122
    [Google Scholar]
  15. Zhao Y., Thomas C., Bremer C., Roy P. 1994; Deletion and mutational analysis of bluetongue virus NS2 protein indicate that the amino but not the carboxy terminus of the protein is critical for RNA-protein interactions. Journal of Virology 68:2179–2185
    [Google Scholar]
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