1887

Abstract

nucleopolyhedrovirus (Ac- MNPV) does not replicate in cells (Bm5, BmN). We have shown previously that when a short DNA sequence within AcMNPV ORF95, which encodes the viral helicase P143, is replaced with the collinear region of nucleopolyhedrovirus (BmNPV), AcMNPV gains the ability to replicate in Bm5 cells. To determine the mutational events in the 143 gene required to allow AcMNPV replication in cells, AcMNPV recombinants produced in Sf9 cells were screened in larvae, which are more permissive to baculo- virus infection than cell lines. Eight combinations of mutations were tested and characterization of viral DNA extracted from dead larvae showed that amino acid changes at position 564 and 577 are required to kill larvae.

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1998-04-01
2022-09-27
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