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Volume 79, Issue 4

Specific sequence changes in the 5′-terminal region of the genome of satellite tobacco mosaic virus are required for adaptation to tobacco mosaic virus. Free

Abstract

The genome of satellite tobacco mosaic virus (STMV) adapted to tobacco mosaic virus (TMV), tomato mosaic virus or green tomato atypical mosaic virus consistently had two single base deletions at positions 1 and 61, corresponding to bases A and G, respectively, as compared to the type-strain genome which is naturally adapted to tobacco mild green mosaic virus (TMGMV). Transcript RNAs (STMV) from clone pSTMV which captured the deletions at positions 1 and 61 were infectious when co-inoculated to tobacco plants with either TMV or TMGMV at infection frequencies of > 90%. Two new STMV variants were created to investigate whether both deletions were essential for adaptation to TMV. These were STMV ΔA, which had the A at position 1 (A) deleted, and STMV ΔG, which lacked G. STMV ΔA was infectious (75% frequency) in the presence of either TMV or TMGMV. Virion RNA of STMV ΔA lost G after one infection cycle with TMV. This deletion did not occur in co-infections with TMGMV. STMV ΔG, like the clone STMV, was infectious (100% frequency) with TMGMV but TMV did not support this clone. When protoplasts were transfected with STMV, STMV ΔA or STMV, STMV replicated when TMGMV was the helper virus. STMVand STMV ΔA replicated in the presence of helper TMV, but STMV did not, the same result as in whole plants. The deletion of A is thus essential for initial STMV adaptation to TMV and the eventual deletion of G is a predicted additional change.

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© Society for General Microbiology 1998
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1998-04-01
2024-04-25
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Specific sequence changes in the 5′-terminal region of the genome of satellite tobacco mosaic virus are required for adaptation to tobacco mosaic virus.
J. Gen. Virol. 79, 905 (1998); https://doi.org/10.1099/0022-1317-79-4-905
/content/journal/jgv/10.1099/0022-1317-79-4-905
/content/journal/jgv/10.1099/0022-1317-79-4-905
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