RT Journal Article SR Electronic(1) A1 Chen, Jiangli A1 Ido, Eiji A1 Jin, Minghao A1 Kuwata, Takeo A1 Igarashi, Tatsuhiko A1 Mizuno, Akiko A1 Koyanagi, Yoshio A1 Hayami, MasanoriYR 1998 T1 Replication of human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus strain mac (SIVmac) and chimeric HIV-1/SIVmac viruses having env genes derived from macrophage-tropic viruses: an indication of different mechanisms of macrophage-tropism in human and monkey cells. JF Journal of General Virology, VO 79 IS 4 SP 741 OP 745 DO https://doi.org/10.1099/0022-1317-79-4-741 PB Microbiology Society, SN 1465-2099, AB To investigate the transferability of macrophage (Mϕ)-tropism among primate lentiviruses, we constructed recombinants of human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus strain mac (SIVmac) and chimeric HIV-1/SIVmac (SHIV) having env region Mϕ-tropic determinants. A recombinant HIV-1 having env partially derived from a Mϕ-tropic HIV-1 strain (JR-FL) replicated in human macrophages but not in monkey macrophages. Conversely, a recombinant SIVmac having env from a Mϕ-tropic strain (SIVmac316) replicated in monkey macrophages but not in human macrophages. A new SHIV (designated NM-3rN/JRFL) carrying the LTR and gag, pol, vif, vpx and nef of SIVmac and vpr, tat, rev, vpu and env of HIV-1 with env partially replaced by that of JR-FL was replication-competent in human macrophages but not in monkey macrophages. These results suggest that the Mϕ-tropic determinant is specific to each host species and that the mechanism of Mϕ-tropism is different between HIV and SIV., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-4-741