@article{mbs:/content/journal/jgv/10.1099/0022-1317-79-4-741, author = "Chen, Jiangli and Ido, Eiji and Jin, Minghao and Kuwata, Takeo and Igarashi, Tatsuhiko and Mizuno, Akiko and Koyanagi, Yoshio and Hayami, Masanori", title = "Replication of human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus strain mac (SIVmac) and chimeric HIV-1/SIVmac viruses having env genes derived from macrophage-tropic viruses: an indication of different mechanisms of macrophage-tropism in human and monkey cells.", journal= "Journal of General Virology", year = "1998", volume = "79", number = "4", pages = "741-745", doi = "https://doi.org/10.1099/0022-1317-79-4-741", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-4-741", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "To investigate the transferability of macrophage (Mϕ)-tropism among primate lentiviruses, we constructed recombinants of human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus strain mac (SIVmac) and chimeric HIV-1/SIVmac (SHIV) having env region Mϕ-tropic determinants. A recombinant HIV-1 having env partially derived from a Mϕ-tropic HIV-1 strain (JR-FL) replicated in human macrophages but not in monkey macrophages. Conversely, a recombinant SIVmac having env from a Mϕ-tropic strain (SIVmac316) replicated in monkey macrophages but not in human macrophages. A new SHIV (designated NM-3rN/JRFL) carrying the LTR and gag, pol, vif, vpx and nef of SIVmac and vpr, tat, rev, vpu and env of HIV-1 with env partially replaced by that of JR-FL was replication-competent in human macrophages but not in monkey macrophages. These results suggest that the Mϕ-tropic determinant is specific to each host species and that the mechanism of Mϕ-tropism is different between HIV and SIV.", }