Induction of protective immunity in chickens vaccinated with infectious bronchitis virus S1 glycoprotein expressed by a recombinant baculovirus. Free

Abstract

A recombinant baculovirus containing the S1 glycoprotein gene of the virulent nephropathogenic KM91 strain of infectious bronchitis virus (IBV) was constructed in order to investigate protective immunity in vaccinated chickens. Results from the protection test were evaluated by re-isolation of virus from the kidneys and tracheas of vaccinated chickens after challenge with strain KM91. After three immunizations, the recombinant S1 (rS1) glycoprotein induced 50% protection of the kidney, whilst inactivated KM91 induced 88% and 50% protection of the kidney and trachea, respectively. In chickens primed with the attenuated H120 vaccine strain, which is heterologous to KM91, the rS1 glycoprotein induced 83% protection of the kidney after two immunizations. Haemagglutination-inhi-bition titres were also increased in chickens immunized with the rS1 glycoprotein after three immunizations, and significantly higher titres were detected after challenge. These data indicate that the expressed rS1 glycoprotein alone can induce a protective immune response as well as an antibody response.

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1998-04-01
2024-03-28
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References

  1. Binns M. M., Boursnell M. E. G., Cavanagh D., Pappin D. J. C., Brown T. D. K. 1985; Cloning and Sequencing of the gene encoding the spike protein of the coronavirus IBV. Journal of General Virology 66:719–726
    [Google Scholar]
  2. Cavanagh D. 1983a; Coronavirus IBV glycopolypeptides: size of their polypeptide moieties and nature of their oligosaccharides. Journal of General Virology 64:1187–1191
    [Google Scholar]
  3. Cavanagh D. 1983b; Coronavirus IBV: further evidence that the surface projections are associated with two glycopolypeptides. Journal of General Virology 64:1787–1791
    [Google Scholar]
  4. Cavanagh D. 1983c; Coronavirus IBV: structural characterization of the spike protein. Journal of General Virology 64:2577–2583
    [Google Scholar]
  5. Cavanagh D., Darbyshire J. H., Davis P., Peters R. W. 1984; Induction of humoral neutralizing and haemagglutination-inhibiting antibody by the spike protein of avian infectious bronchitis virus. Avian Pathology 13:573–583
    [Google Scholar]
  6. Cavanagh D., Davis P. J., Darbyshire J. H., Peters R. W. 1986; Coronavirus IBV: virus retaining spike glycopolypeptide S2 but not S1 is unable to induce virus-neutralizing or haemagglutination-inhibiting antibody, or induce chicken tracheal protection. Journal of General Virology 67:1435–1442
    [Google Scholar]
  7. Cavanagh D., Davis P. J., Cook J. K. A., Li D., Kant A., Koch G. 1992; Location of the amino acid differences in the S1 spike glycoprotein subunit of closely related serotypes of infectious bronchitis virus. Avian Pathology 21:33–43
    [Google Scholar]
  8. Endo-Munoz L. B., Faragher J. T. 1989; Avian infectious bronchitis : cross-protection studies using different Australian subtypes. Australian Veterinary Journal 66:345–348
    [Google Scholar]
  9. Ignjatovic J., Galli L. 1994; The S1 glycoprotein but not the N or M proteins of avian infectious bronchitis virus induces protection in vaccinated chickens. Archives of Virology 138:117–134
    [Google Scholar]
  10. Kant A., Koch G., van Roozelaar D. J., Kusters J. G., Poelwijk F. A. J., van der Zeijst A. M. 1992; Location of antigenic sites defined by neutralizing monoclonal antibodies on the S1 avian infectious bronchitis virus glycopolypeptide. Journal of General Virology 73:591–596
    [Google Scholar]
  11. King D. J., Cavanagh D. 1991; Infectious bronchitis. In Diseases of Poultry, 9th edn. pp. 471–484 Calnek B. W., Barnes H. J., Beard C. W., Reid W. M., Yoder H. W. Jr Edited by Ames, Iowa: Iowa State University Press;
    [Google Scholar]
  12. King D. J., Hopkins S. R. 1983; Evaluation of the hemagglutination-inhibition test for measuring the response of chickens to avian infectious bronchitis virus vaccination. Avian Diseases 27:100–112
    [Google Scholar]
  13. Kitts P. A., Ayres M. D., Possee R. D. 1990; Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Research 18:5667–5672
    [Google Scholar]
  14. Koch G., Hartog L., Kant A., van Roozelaar D. J. 1990; Antigenic domains on the peplomer protein of avian infectious bronchitis virus : correlation with biological functions. Journal of General Virology 71:1929–1935
    [Google Scholar]
  15. Kusters J. G., Niesters H. G. M., Bleumink-Pluym N. M. C., Davelaar F. G., Horzinek M. C., van der Zeijst B. A. M. 1987; Molecular epidemiology of infectious bronchitis virus in the Netherlands. Journal of General Virology 68:343–352
    [Google Scholar]
  16. Kwon H. M., Jackwood M. W., Gelb J. Jr 1993; Differentiation of infectious bronchitis virus serotypes using polymerase chain reaction and restriction fragment length polymorphism analysis. Avian Diseases 37:194–202
    [Google Scholar]
  17. Lambrechts C., Pensaert M., Ducatelle R. 1993; Challenge experiments to evaluate cross-protection induced at the trachea and kidney level by vaccine strains and Belgian nephropathogenic isolates of avian infectious bronchitis virus. Avian Pathology 22:577–590
    [Google Scholar]
  18. Mockett A. P. A., Cavanagh D., Brown T. D. K. 1984; Monoclonal antibodies to the S1 spike and membrane proteins of avian infectious bronchitis coronavirus strain Massachusetts M41. Journal of General Virology 65:2281–2286
    [Google Scholar]
  19. Muneer M. A., Newman J. A., Halvorsan D. A., Sivanandan V., Nagaraja K. V., Coon N. 1988; Efficacy ofinfectious bronchitis virus vaccines against heterologous challenge. Research in Veterinary Science 45:22–27
    [Google Scholar]
  20. Picault J. P., Drouin P., Guittet M., Bennejean G., Protais J., L’Hospitalier R., Gillet J. P., Lamande J., Bachelier A. L. 1986; Isolation, characterization and preliminary cross protection studies with a new pathogenic avian infectious bronchitis virus (strain PL-84084). Avian Pathology 15:367–383
    [Google Scholar]
  21. Song C.-S., Kim J. H., Lee Y. J., Kim S. J., Izumiya Y., Tohya Y., Jang H. K., Mikami T. 1998; Detection and classification of infectious bronchitis virus isolated in Korea by dot-immunoblotting assay using monoclonal antibodies. Avian Diseases 42:1651–1670
    [Google Scholar]
  22. Tomley F. M., Mockett A. P. A., Boursnell M. E. G., Binns M. M., Cook J. K. A., Brown T. D. K., Smith G. L. 1987; Expression of the infectious bronchitis virus spike protein by recombinant vaccinia virus and induction of neutralizing antibodies in vaccinated mice. Journal of General Virology 68:2291–2298
    [Google Scholar]
  23. Wang L., Junker D., Collisson E. W. 1993; Evidence of natural recombination within the S1 gene of infectious bronchitis virus. Virology 192:710–716
    [Google Scholar]
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