1887

Abstract

The gene encoding the major occlusion body protein, spheroidin, of entomopoxvirus (AmEPV) was introduced into a baculovirus vector under control of the polyhedrin gene promoter. A recombinant virus produced large, ovoid occlusion body-like structures in both and cells. These structures resembled the spheroids found in AmEPV- infected cells, except they were devoid of virus particles and were not surrounded by a membrane- or envelope-like structure. These results were confirmed by immunofluoresence microscopy and Western blotting using a specific antipeptide antibody to spheroidin, and suggest that the supramolecular assembly of spheroids is not dependent on other EPV-encoded gene products. Transmission electron microscopy and subcellular fractionation experiments revealed that the spheroid-like structures were assembled in both the nucleus and cytoplasm of the recombinant virus- infected cells. This contrasts with the solely cytoplasmic localization found in AmEPV-infected cells.

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1998-03-01
2022-01-27
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