1887

Journal of General Virology header logo

Volume 79, Issue 2

Sequence and in vivo transcription of Lacanobia oleracea granulovirus egt Free

Abstract

We have determined the nucleotide sequence and located the major transcript termini of the granulovirus (LoGV) gene. The open reading frame encodes a 460-amino acid polypeptide having extensive sequence similarity to ten nucleopolyhedrovirus (NPV) ecdysteroid UDP- glucosyltransferase (EGT) proteins; the degree of similarity is particularly high within several previously identified EGT‘domains’, and eight invariant amino acid residues are conserved. A phylogenetic tree, constructed by the neighbour joining method, showed LoGV EGT to be the most highly diverged of the eleven baculovirus sequences compared. Database searching revealed that part of a published DNA sequence from granulovirus appears to encode the N-terminal region of EGT, and the relative genomic locations of the and genes in that virus were compared with their positions in LoGV. In infected larvae, is transcribed predominantly as a1 6 kb mRNA. Primer extension analysis suggested that the major transcription terminus is located within a baculovirus late gene promoter motif (GTAAG), in contrast to the early gene promoter contexts determined by others for three NPV mRNA 5′ ends. An early transcriptional start site is also used in LoGV expression, but at a much lower level. The 3′ terminus of mRNA was identified by sequencing DNA fragments generated by rapid amplification of cDNA ends, and is located 58–62 nucleotides beyond the translation stop codon.

  • Published Online:
© Society for General Microbiology 1998
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-2-405
1998-02-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/2/9472627.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-2-405&mimeType=html&fmt=ahah

References

  1. Barrett J. W., Krell P. J., Arif B. M. 1995; Characterization, sequencing and phylogeny of the ecdysteroid UDP-glucosyltransferase gene from two distinct nuclear polyhedrosis viruses isolated from Choristoneura fumiferana . Journal of General Virology 76:2447–2456
     [Google Scholar]
  2. Bonning B. C., Hammock B. D. 1996; Development of recombinant baculoviruses for insect control. Annual Review of Entomology 41:191–210
     [Google Scholar]
  3. Blissard G. W., Rohrmann G. F. 1989; Location, sequence, transcriptional mapping, and temporal expression of the gp64 envelope glycoprotein gene of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus. Virology 170:537–555
     [Google Scholar]
  4. Burand J. P., Park E. J., Kelly T. J. 1996; Dependence of ecdysteroid metabolism and development in host larvae on the time of baculovirus infection and the activity of the UDP-glucosyl transferase gene. Insect Biochemistry and Molecular Biology 26:845–852
     [Google Scholar]
  5. Clarke E. E., Tristem M., Cory J. S., O’Reilly D. R. 1996; Characterization of the ecdysteroid UDP-glucosyltransferase gene from Mamestra brassicae nucleopolyhedrovirus. Journal of General Virology 77:2865–2871
     [Google Scholar]
  6. Corbitt T. S., Bryning G., Olieff S., Edwards J. P. 1996; Reproductive, developmental and nutritional biology of the tomato moth, Lacanobia oleracea (Lepidoptera: Noctuidae) reared on artificial diet. Bulletin of Entomological Research 86:647–657
     [Google Scholar]
  7. Crook N. E. 1991; Baculoviridae : subgroup B : comparative aspects of granulosis viruses. In Viruses of Invertebrates pp 73–110 Kurstak E. Edited by New York: Marcel Dekker;
     [Google Scholar]
  8. Crook N. E., Brown J. D., Foster G. N. 1982; Isolation and characterization of a granulosis virus from the tomato moth, Lacanobia oleracea, and its potential as a control agent. Journal of Invertebrate Pathology 40:221–227
     [Google Scholar]
  9. Engelhard E. K., Volkman L. E. 1995; Developmental resistance in fourth instar Trichoplusia ni inoculated with Autographa californica M nuclear polyhedrosis virus. Virology 209:384–389
     [Google Scholar]
  10. Faktor O., Toister-Achituv M., Kamensky B. 1995; Identification and nucleotide sequence of an ecdysteroid UDP-glucosyltransferase gene of Spodoptera littoralis multicapsid nuclear polyhedrosis virus. Virus Genes 11:47–52
     [Google Scholar]
  11. Frohman M. A. 1990; RACE : rapid amplification of cDNA ends. In PCR Protocols pp 28–38 Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. Edited by London: Academic Press;
     [Google Scholar]
  12. Hu Z. H., Broer R., Westerlaken J., Martens J. W. M., Jin F., Jehle J. A., Wang L. M., Vlak J. M. 1997; Characterization of the ecdysteroid UDP-glucosyltransferase gene of a single nucleocapsid nucleopoly- hedrovirus of Buzura suppressaria . Virus Research 47:91–97
     [Google Scholar]
  13. Huber J., Hughes P. R. 1984; Quantitative bioassay in insect pathology. Bulletin of the Entomological Society of America 30:31–34
     [Google Scholar]
  14. Jarvis D. J., Garcia A. 1994; Biosynthesis and processing of the Autographa californica nuclear polyhedrosis virus gp64 protein. Virology 205:300–313
     [Google Scholar]
  15. Jehle J. A., Backhaus H. 1994; The granulin gene region of Cryptophlebia leucotreta granulosis virus: sequence analysis and phylogenetic considerations. Journal of General Virology 75:3667–3671
     [Google Scholar]
  16. Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. editors 1995 Virus Taxonomy. Sixth Report ofthe International Committee on Taxonomy of Viruses Vienna & New York: Springer-Verlag;
     [Google Scholar]
  17. O’Reilly D. R. 1995; Baculovirus-encoded ecdysteroid UDP-glucosyl- transferases. Insect Biochemistry and Molecular Biology 25:541–550
     [Google Scholar]
  18. O’Reilly D. R., Miller L. K. 1989; A baculovirus blocks insect molting by producing ecdysteroid UDP-glucosyl transferase. Science 245:1110–1112
     [Google Scholar]
  19. O’Reilly D. R., Miller L. K. 1990; Regulat ion of expression of a baculovirus ecdysteroid UDP-glucosyltransferase gene. Journal of Virology 64:1321–1328
     [Google Scholar]
  20. O’Reilly D. R., Miller L. K. 1991; Improvement of a baculovirus pesticide by deletion of the egt gene. Bio/Technology 9:1086–1089
     [Google Scholar]
  21. O’Reilly D. R., Brown M. R., Miller L. K. 1992; Alteration of ecdysteroid metabolism due to baculovirus infection of the fall armyworm Spodoptera frugiperda: host ecdysteroids are conjugated with galactose. Insect Biochemistry and Molecular Biology 22:313–320
     [Google Scholar]
  22. Riegel C. I., Lanner-Herrera C., Slavicek J. M. 1994; Identification and characterization of the ecdysteroid UDP-glucosyltransferase gene of the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus. Journal of General Virology 75:829–838
     [Google Scholar]
  23. Saitou N., Nei M. 1987; The neighbor joining method : a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425
     [Google Scholar]
  24. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  25. Smith I. R. L., Crook N. E. 1988; In vivo isolation of baculovirus genotypes. Virology 166:240–244
     [Google Scholar]
  26. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
     [Google Scholar]
  27. Toister-Achituv M., Faktor O. 1997; Transcriptional analysis and promoter activity of the Spodoptera littoralis multicapsid nucleopoly- hedrovirus ecdysteroid UDP-glucosyltransferase gene. Journal of General Virology 78:487–491
     [Google Scholar]
  28. van Wezel G. P., White J., Young P., Postma P. W., Bibb M. J. 1997; Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacI-galR family of regulatory genes. Molecular Microbiology 23:537–549
     [Google Scholar]
  29. Wang L.-M., Weber D. K., Johnson T., Sakaguchi A. Y. 1988; Supercoil sequencing using unpurified templates produced by rapid boiling. BioTechniques 6:839–843
     [Google Scholar]
  30. Winstanley D., Crook N. E. 1993; Replication of Cydia pomonella granulosisvirus in cell cultures. Journal of General Virology 74:1599–1609
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-2-405
Loading
Sequence and in vivo transcription of Lacanobia oleracea granulovirus egt
J. Gen. Virol. 79, 405 (1998); https://doi.org/10.1099/0022-1317-79-2-405
/content/journal/jgv/10.1099/0022-1317-79-2-405
/content/journal/jgv/10.1099/0022-1317-79-2-405
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error