1887

Abstract

The largest open reading frame of porcine circovirus (ORF 4) encodes a protein of 312 amino acids. The predicted gene product of ORF 4 shows similarities to Rep proteins of other plant circo- viruses and geminiviruses. Three motifs have been identified that are characteristic for proteins involved in rolling circle replication and the consensus sequence for a putative dNTP-binding box (GKS) has been found. In this paper, experimental evidence is presented which indicates that ORF 4 encodes the replication protein of porcine circovirus. After cloning of the ORF 4 gene product, it was supplied in a transient replication assay. The ORF 4 gene product promoted the replication of plasmid pOP11, which carries the origin of DNA replication of porcine circovirus. Since pOP11 itself is unable to replicate in virus-free porcine kidney cells, the ORF 4 gene product must be essential for replication of porcine circovirus.

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/content/journal/jgv/10.1099/0022-1317-79-2-381
1998-02-01
2022-08-15
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References

  1. Boevink P., Chu P. W. G., Keese P. 1995; Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses. Virology 207:354–36l
    [Google Scholar]
  2. DeLange A. M., McFadden G. 1986; Sequence-nonspecific replication of transfected plasmid DNA in poxvirus-infected cells. Proceedings of the National Academy of Sciences, USA 83:614–618
    [Google Scholar]
  3. Harding R. M., Burns T. M., Hafner G., Dietzgen R. G., Dale J. L. 1993; Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene. Journal of General Virology 74:323–328
    [Google Scholar]
  4. Heyraud F., Matzeit V., Kammann M., Schaefer S., Schell J., Gronenborn B. 1993; Identification of the initiation sequence for viral-strand DNA synthesis of wheat dwarf virus. EMBO Journal 12:4445–4452
    [Google Scholar]
  5. Hirt B. 1967; Selective extraction of polyomavirus DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  6. Koonin E. V., Ilyina T. V. 1993; Computer assisted dissection of rolling circle DNA replication. BioSystems 30:241–268
    [Google Scholar]
  7. Lazarowitz S. 1992; Geminiviruses: genome structure and gene function. Critical Reviews in Plant Sciences 11:327–349
    [Google Scholar]
  8. Mankertz A., Persson F., Mankertz J., Blaess G., Buhk H.-J. 1997; Mapping and characterization of the origin of DNA replication of porcine circovirus. Journal of Virology 71:2562–2566
    [Google Scholar]
  9. Meehan B. M., Creelan J. L., McNulty M. S., Todd D. 1997; Sequence of porcine circovirus DNA: affinities with plant circoviruses. Journal of General Virology 78:221–227
    [Google Scholar]
  10. Pansegrau W., Lanka E. 1996; Enzymology of DNA transfer by conjugative mechanisms. Progress in Nucleic Acid Research and Molecular Biology 54:197–251
    [Google Scholar]
  11. Ritchie B. W., Niagro F. D., Lukert P. D., Steffens W. L., Latimer K. S. 1989; Characterization of a new virus from cockatoos with psittacine beak and feather disease virus. Virology 171:83–88
    [Google Scholar]
  12. Rohde W., Randles J. W., Langridge P., Hanold D. 1990; Nucleotide sequence of a circular single-stranded DNA associated with coconut foliar decay virus. Virology 176:648–651
    [Google Scholar]
  13. Stanley J. 1995; Analysis of African cassava mosaic virus recombinants suggests strand nicking occurs within the conserved nona- nucleotide motif during the initiation of rolling circle DNA replication. Virology 206:707–712
    [Google Scholar]
  14. Tischer I., Rasch R., Tochtermann G. 1974; Characterization of papovavirus- and picornavirus-like particles in permanent pig kidney cell lines. Zentralblatt fur Bakteriologie Mikrobiologie und Hygiene Series A 226:153–167
    [Google Scholar]
  15. Tischer I., Gelderblom H., Vettermann W., Koch M. A. 1982; A very small porcine virus with circular single-stranded DNA. Nature 295:64–66
    [Google Scholar]
  16. Todd D., Creelan J. L., Mackie D. P., Rixon F., McNulty M. S. 1990; Purification and biochemical characterization of chicken anaemia agent. Journal of General Virology 71:819–823
    [Google Scholar]
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