1887

Abstract

To gain more insight into the structure of the African horsesickness virus (AHSV) core particle, we have cloned, partially characterized and expressed the two major core proteins, VP3 and VP7, of AHSV-9. VP7 was found to be highly conserved amongst different serotypes. The VP3 and VP7 genes were subsequently expressed in insect cells by means of recombinant baculoviruses. VP7 was synthesized to very high levels and aggregated into distinctive crystals. Co-expression of VP3 and VP7 resulted in the intracellular formation of core-like particles which structurally resembled empty AHSV cores.

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1998-02-01
2024-04-16
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References

  1. Bremer C. W. 1976; A gel electrophoretic study of the protein and nucleic acid components of African horsesickness virus. Onderstepoort Journal of Veterinary Research 43:193–200
    [Google Scholar]
  2. Bremer C. W., Huismans H., Van Dijk A. A. 1990; Characterization and cloning of the African horsesickness virus genome. Journal of General Virology 71:793–799
    [Google Scholar]
  3. Burrage T. G., Trevejo R., Stone-Marschat M., Laegreid W. W. 1993; Neutralizing epitopes of African horsesickness virus serotype 4 are located on VP2. Virology 196:799–803
    [Google Scholar]
  4. Burroughs J. N., O’Hara R. S., Smale C. J., Hamblin C., Walton A., Armstrong R. 1994; Purification and properties of virus particles, infectious subviral particles, cores and VP7 crystals of African horse- sickness virus serotype 9. Journal of General Virology 75:1849–1857
    [Google Scholar]
  5. Chuma T., LeBlois H., Sanchez-Vizcaino J. M., Diaz-Laviada M., Roy P. 1992; Expression of the major core antigen VP7 of African horsesickness virus by a recombinant baculovirus and its use as a group- specific diagnostic agent. Journal of General Virology 73:925–931
    [Google Scholar]
  6. French T. J., Roy P. 1990; Synthesis of bluetongue virus (BTV) corelike particles by a recombinant baculovirus expressing the two major structural core proteins of BTV. Journal of Virology 64:1530–1536
    [Google Scholar]
  7. French T. J., Marshall J. J. A., Roy P. 1990; Assembly of doubleshelled viruslike particles of bluetongue virus by simultaneous expression of four structural proteins. Journal of Virology 64:5695–5700
    [Google Scholar]
  8. Grimes J., Basak A. K., Roy P., Stuart D. 1995; The crystal structure of bluetongue virus VP7. Nature 373:167–170
    [Google Scholar]
  9. Hewat E. A., Booth T. F., Loudon P. T., Roy P. 1992; Three dimensional reconstruction of baculovirus expressed bluetongue virus core-like particles by cryo-electron microscopy. Virology 189:10–20
    [Google Scholar]
  10. Iwata H., Yamagawa M. S., Roy P. 1992; Evolutionary relationships among the gnat-transmitted orbiviruses that cause African horsesickness, bluetongue and epizootic haemorrhagic disease as evidenced by their capsid protein sequences. Virology 191:251–261
    [Google Scholar]
  11. Liu H. M., Booth T. F., Roy P. 1992; Interactions between bluetongue virus core and capsid proteins translated in vitro . Journal of General Virology 73:2577–2584
    [Google Scholar]
  12. Luckow V. A., Lee S. C., Barry G. F., Olins P. O. 1993; Efficient generation of infectious recombinant baculoviruses by site-specific transposon mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli . Journal of Virology 67:4566–4579
    [Google Scholar]
  13. Martinez-Torrecuadrada J. L., Casal J. I. 1995; Identification of a linear neutralization domain in the protein VP2 of African horsesickness virus. Virology 210:391–399
    [Google Scholar]
  14. Prasad B. V. V., Yamaguchi S., Roy P. 1992; Three-dimensional structure of single-shelled bluetongue virus. Journal of Virology 66:2135–2142
    [Google Scholar]
  15. Roy P. 1992; Bluetongue virus proteins. Journal of General Virology 73:3051–3064
    [Google Scholar]
  16. Roy P., Hirasawa T., Fernandez M., Blinov V. M., Sanchez-Vixcain Rodrique J. M. 1991; The complete sequence of the group-specific antigen, VP7, of African horsesickness virus serotype 4 reveals a close relationship to bluetongue virus. Journal of General Virology 72:1237–1241
    [Google Scholar]
  17. Roy P., Mertens P. P. C., Casal I. 1994; African horse sickness virus structure. Comparative Immunology and Microbiology of Infectious diseases 17:243–273
    [Google Scholar]
  18. Van Wyngaardt W., Du Plessis D. H., Van Wyngaardt S., Verschoor J. A. 1992; Production and properties of monoclonal antibodies against African horsesickness virus, serotype 3. Onderstepoort Journal of Veterinary Research 59:129–133
    [Google Scholar]
  19. Vreede F. T., Huismans H. 1994; Cloning, characterization and expression of the gene that encodes the major neutralization-specific antigen of African horsesickness virus serotype 3. Journal of General Virology 75:3629–3633
    [Google Scholar]
  20. Wade-Evans A. M., Woolhouse T., O’Hara R., Hamblin C. 1993; The use of African horsesickness virus VP7 antigen, synthesized in bacteria and anti-VP7 monoclonal antibodies in a competitive ELISA. Journal of Virological Methods 45:179–188
    [Google Scholar]
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