1887

Abstract

Antibody-forming cell (AFC) responses in the nasal- associated lymphoid tissue (NALT) of BALB/c mice were examined following intranasal infection, mainly of the upper respiratory tract, with a small volume of influenza virus. The infection induced significant accumulation of T and B cells in NALT, peaking around day 7 post-infection. Virus-specific IgA, IgG and IgM AFC responses were induced, developing from day 5 and peaking at day 7; responses were predominantly IgA and IgG, followed by IgM. At peak, NALT contained the greatest number of IgA AFCs per total cells of the lymphoid tissues examined in the upper respiratory tract. The IgM AFC responses were induced in NALT cell cultures from uninfected mice following culture with influenza virus, indicating that at least a part of the AFCs in infected mice may have originated from specific B cell precursors in NALT. In parallel with the detection of AFCs in infected mice, virus-specific IgA antibodies appeared in the nasal wash and their appearance correlated well with virus clearance from the nasal area. These results suggest that virus-specific IgA antibodies, produced by IgA AFCs in NALT, play an important role in recovery from infection.

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1998-02-01
2024-03-29
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References

  1. Asanuma H., Inaba Y., Aizawa C., Kurata T., Tamura S.-I. 1995; Characterization of mouse nasal lymphocytes isolated by enzymatic extraction with collagenase. Journal of Immunological Methods 187:41–51
    [Google Scholar]
  2. Asanuma H., Thompson A. H., Iwasaki T., Sato Y., Inaba Y., Aizawa C., Kurata T., Tamura S.-I. 1997; Isolation and characterization of mouse nasal-associated lymphoid tissue. Journal ofImmunological Methods 202:123–131
    [Google Scholar]
  3. Balkovic E. S., Six H. R. 1986; Pulmonary and serum isotypic antibody response of mice to live and inactivated influenza virus. American Review of Respiratory Diseases 134:6–11
    [Google Scholar]
  4. Cher D. J., Mosmann T. R. 1987; Two types of murine helper T cell clone. II. Delayed-type hypersensitivity is mediated by Th1 clones. Journal of Immunology 138:3688–3694
    [Google Scholar]
  5. Corthier G., Vannier P. 1983; Production of coproantibodies and immune complexes in piglets infected with rotavirus. Journal of Infectious Diseases 147:293–301
    [Google Scholar]
  6. Czerkinsky C., Nilsson L. A., Nygren H., Ouchterlony O., Tarkowski A. 1983; A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. Journal of Immunological Methods 65:109–121
    [Google Scholar]
  7. Davenport F. M., Hennessy A. V., Brandon F. M., Webster R. G., Barrett C. D.Jr Lease G. O. 1964; Comparisons of serologic and febrile responses in humans to vaccination with influenza A viruses or their hemagglutinins. Journal ofLaboratory and Clinical Medicine 63:5–13
    [Google Scholar]
  8. Eichelberger M., Allan W., Zijlstra M., Jaenisch R., Doherty P. C. 1991; Clearance of influenza virus respiratory infection in mice lacking class I major histocompatibility complex-restricted CD8+ T cells. Journal of Experimental Medicine 174:875–880
    [Google Scholar]
  9. Jones P. D., Ada G. L. 1986; Influenza virus-specific antibody- secreting cells in the murine lung during primary influenza virus infection. Journal of Virology 60:614–619
    [Google Scholar]
  10. Justewicz D. M., Morin M. J., Robinson H. L., Webster R. G. 1995; Antibody-forming cell response to virus challenge in mice immunized with DNA encoding the influenza virus hemagglutinin. Journal of Virology 69:7712–7717
    [Google Scholar]
  11. Koornstra P. J., DeJong F. I. C. R. S., Vlek L. M. K., Marres E. H. M. A. 1991; The Waldeyer ring equivalent in the rat. Acta Otolaryngologica 111:591–599
    [Google Scholar]
  12. Kris R. M., Asofsky R., Evans C. B., > Small P. A.Jr 1985; Protection and recovery in influenza virus-infected mice immuno- suppressed with anti-IgM. Journal of Immunology 134:1230–1235
    [Google Scholar]
  13. Kuper C. F., Koornastra P. J., Hameleers D. M. H., Biewenga J., Spit B. J., Duijvestijn A. M., Van Breda Vriesman P. J. C., Sminia T. 1992; The role of nasopharyngeal lymphoid tissue. Immunology Today 13:219–224
    [Google Scholar]
  14. Lightman S., Cobbold S., Waldmann H., Askonas B. A. 1987; Do L3T4+ T cells act as effector cells in protection against influenza virus infection?. Immunology 62:139–144
    [Google Scholar]
  15. Lycke N. 1986; A sensitive method for the detection of specific antibody production in different isotypes from single lamina propria plasma cells. Scandanavian Journal of Immunology 24:393–403
    [Google Scholar]
  16. Mayer H. M.Jr Hopps H. E., Parleman P. D., Ennis F. A. 1978; .Review of existing vaccines for influenza. American Journal of Clinical Pathology 70:146–152
    [Google Scholar]
  17. McMichael A. 1994; Cytotoxic T lymphocytes specific for influenza virus. Current Topics in Microbiology & Immunology 189:75–91
    [Google Scholar]
  18. Mosmann R. R., Coffman R. L. 1989; Th1 and Th2 cells: different patterns of lymphokine secretion lead to different functional properties. Annual Review of Immunology 7:145–173
    [Google Scholar]
  19. Murphy B. R., Webster R. G. 1996; Orthomyxoviruses. In Fields Virology pp 1397–1445 Fields B. N., Knipe D. M., Howley P. M., Chanock R. M., Hirsch M. S., Melnick J. L., Monath T. P., Roizman B., Straus S. E. Edited by Philadelphia: Lippincott-Raven;
    [Google Scholar]
  20. Outlaw M. C., Dimmock N. J. 1990; Mechanisms of neutralization of influenza virus on mouse tracheal epithelial cells by mouse monoclonal polymeric IgA and polyclonal IgM directed against the viral haem- agglutinin. Journal of General Virology 71:69–76
    [Google Scholar]
  21. Outlaw M. C., Dimmock N. J. 1991; Insights into neutralization of animal viruses gained from study of influenza virus. Epidemiology and Infection 106:205–220
    [Google Scholar]
  22. Phelan M. A., Mayner R. E., Bucker D. J., Ennis F. A. 1980; Purification of influenza virus glycoproteins for the preparation and standardization of immunological potency testing reagents. Journal of Biological Standardization 8:233–242
    [Google Scholar]
  23. Renegar K. B., Small P. A.Jr 1991; Passive transfer of local immunity to influenza virus infection by IgA antibody. Journal of Immunology 146:1972–1978
    [Google Scholar]
  24. Spit B. J., Hendriksen E. G. J., Bruijntjes J. P., Kuper C. F. 1989; Nasal lymphoid tissue in the rat. Cell Tissue Research 255:193–198
    [Google Scholar]
  25. Stavitsky A. B. 1954; Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein- treated red blood cells. Journal of Immunology 72:360–367
    [Google Scholar]
  26. Tamura S.-I., Kojima A., Egashira Y. 1981; Regulatory mechanism of delayed-type hypersensitivity in mice. III. In vitro analysis of memory T cells involved in augmentation of DTH responses. Microbiology and Immunology 25:595–609
    [Google Scholar]
  27. Tamura S.-I., Samegai Y., Kurata H., Nagamine T., Aizawa C., Kurata T. 1988; Protection against influenza virus infection by vaccine inoculated intranasally with cholera toxin B subunit. Vaccine 6:409–413
    [Google Scholar]
  28. Tamura S.-I., Funato H., Hirabayashi Y., Suzuki Y., Nagamine T., Aizawa C., Kurata T. 1991; Cross-protection against influenza A virus infection by passively transferred respiratory tract IgA antibodies to different hemagglutinin molecules. European Journal of Immunology 21:1337–1344
    [Google Scholar]
  29. Tamura S.-I., Asanuma H., Ito Y., Hirabayashi Y., Suzuki Y., Nagamine T., Aizawa C., Kurata T., Oya A. 1992a; Superior crossprotective effect of nasal vaccination of subcutaneous inoculation with influenza HA vaccine. European Journal of Immunology 22:477–481
    [Google Scholar]
  30. Tamura S.-I., Ito Y., Asanuma H., Hirabayashi Y., Suzuki Y., Nagamine T., Aizawa C., Kurata T. 1992b; Cross-protection against influenza virus infection afforded by trivalent inactivated vaccines inoculated intranasally with cholera toxin B subunit. Journal ofImmunology 149:981–988
    [Google Scholar]
  31. Tamura S.-I., Miyata K., Matsuo K., Asanuma H., Takahashi H., Nakajima K., Suzuki Y., Aizawa C., Kurata T. 1996; Acceleration of influenza virus clearance by Th1 cells in the nasal site of mice immunized intranasally with adjuvant-combined recombinant nucleo- protein. Journal of Immunology 156:3892–3900
    [Google Scholar]
  32. Tilney N. L. 1971; Patterns of lymphatic drainage in the adult laboratory rat. Journal of Anatomy 109:369–383
    [Google Scholar]
  33. van der Ven I., Sminia T. 1993; The development and structure of mouse nasal associated lymphoid tissue: an immuno-and enzyme- histochemical study. Regional Immunology 5:69–75
    [Google Scholar]
  34. Wilson I. R., Cox J. C. 1990; Structural basis of immune recognition of influenza virus hemagglutinin. Annual Review of Immunology 8:737–771
    [Google Scholar]
  35. Yap K. L., Ada G. L., MaKensie I. F. C. 1978; Transfer of specific cytotoxic T lymphocytes protects mice inoculated with influenza virus. Nature 273:238–239
    [Google Scholar]
  36. Yetter R. A., Lehrer S., Ramphal R., Small P. A.Jr 1980; Outcome of influenza infection: effect of site of initial infection and heterotypic immunity. Infection and Immunity 29:654–662
    [Google Scholar]
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