1887

Abstract

Transcription of human immunodeficiency virus type 1 (HIV-1) is regulated by a series of host DNA-binding proteins which interact with the upstream viral promoter sequences, and the viral regulatory protein Tat which associates with TAR RNA sequences located in the leader of the viral transcript. Here we have examined the ability of the cellular protein YB-1 to modulate transcription of the HIV-1 promoter in a human astrocytic cell line (U-87MG), a neuronal cell line (SK-N-MC) and lymphoid cells (Jurkat) by transfection assay. Ectopic expression of YB-1 in U-87MG and SK-N-MC augments basal transcriptional activity of the viral sequence located between 80 and 43, which encompasses the GC-rich motif. In accord with the previous report, in Jurkat cells YB-1-mediated activation of the HIV-1 promoter required sequences which are located further upstream from the GC-rich motif. Combined overexpression of YB-1 and the GC-rich binding protein, SP1, in the transfected cells decreased the level of activation of the viral promoter, suggesting that YB-1 and SP1 may exert negative effects on each other’s function. Results from band shift assay with purified YB-1 and SP1 indicated that SP1 and YB-1 bind to the GC-rich DNA sequence in the double-stranded and single-stranded configurations, respectively. However, efficient binding of SP1 to the double-stranded GC-rich motif corresponding to the HIV-1 long terminal repeat (LTR) is diminished in the presence of YB-1. Similarly, in the presence of SP1, YB-1 loses its ability to become associated with its target single-stranded DNA probe. No evidence for direct association of YB-1 and SP1 either in the presence or in the absence of DNA was observed. These data suggest that while YB-1 stimulates expression of the LTR in central nervous system cells, the level of activity of other cellular proteins, such as SP1, may dictate binding of YB-1 to its target sequence, and therefore affect the regulatory function of this protein.

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1998-02-01
2022-05-23
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References

  1. Barberis A., Superti-Furga G., Busslinger M. 1987; Mutually exclusive interaction of the CCAAT-binding factor and of a displacement protein with overlapping sequences of a histone gene promoter. Cell 50:347–359
    [Google Scholar]
  2. Benoist C., Mathis D. 1990; Regulat ion of major histocompatibility complex class II genes: X, Y, and other letters of the alphabet. Annual Review of Immunology 8:681–715
    [Google Scholar]
  3. Chen N. N., Chang C.-F., Gallia G. L., Kerr D. A., Johnson E. M., Krachmarov C. P., Barr S. M., Frisque R. J., Bollag B., Khailili K. 1995; Cooperative action of cellular proteins, YB-1 and Pura with the tumor antigen of the human JC polyomavirus determines their interaction with the viral lytic control element. Proceedings of the National Academy of Sciences, USA 92:1087–1091
    [Google Scholar]
  4. Cohen I., Reynolds W. F. 1991; The Xenopus YB3 protein binds the B box element of the class III promoter. Nucleic Acids Research 19:4753–4759
    [Google Scholar]
  5. Didier D. K., Schiffenbauer J., Woulfe S. L., Zacheis M., Schwartz B. D. 1988; Characterization of the cDNA encoding a protein binding to the major histocompatibility complex class II Y box. Proceedings of the National Academy of Sciences, USA 85:7322–7326
    [Google Scholar]
  6. Dorn A., Durand B., Marfing C., LeMeur M., Benoist C., Mathis D. 1987; Conserved major histocompatibility complex class II boxes -X and Y -are transcriptional control elements and specfically bind nuclear proteins. Proceedings of the National Academy of Sciences, USA 84:6249–6253
    [Google Scholar]
  7. Feng S., Holland E. C. 1988; HIV-1 tat trans-activation requires the loop sequence within TAR. Nature 334:165–167
    [Google Scholar]
  8. Garcia J. A., Gaynor R. B. 1994; The human immunodeficiency virus type 1 long terminal repeat and its role in gene expression. Progress in Nucleic Acid Research and Molecular Biology 49:157–196
    [Google Scholar]
  9. Garcia J. A., Wu F. K., Mitsuyasu R., Gaynor R. B. 1987; Interactions of cellular proteins involved in the transcriptional regulation of the human immunodeficiency virus. EMBO Journal 6:3761–3770
    [Google Scholar]
  10. Gaynor R. 1992; Cellular transcription factors involved in the regulation of HIV-1 gene expression. AIDS 6:347–363
    [Google Scholar]
  11. Gaynor R. B. 1995; Regulat ion of HIV-1 gene expression by the transactivator protein Tat. Current Topics in Microbiological Immunology 193:51–77
    [Google Scholar]
  12. Gorman C. M., Moffat L. F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyl transferases in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  13. Graham F. L., van der Eb A. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–457
    [Google Scholar]
  14. Gualberto A., Baldwin A. S.Jr 1995; p53 and Sp1 interact and cooperate in the tumor necrosis factor-induced transcriptional activation of the HIV-1 long terminal repeat. Journal of Biological Chemistry 270:19680–19683
    [Google Scholar]
  15. Harrich D., Garcia J., Wu F., Mitsuyasu R., Gonzalez J., Gaynor R. 1989; Role of SP1-binding domains in in vivo transcriptional regulation of the human immunodeficiency virus type 1 long terminal repeat. Journal of Virology 63:2585–2591
    [Google Scholar]
  16. Harrich D., Ulich C., Gaynor R. B. 1996; A critical role for the TAR element in promoting efficient human immunodeficiency virus type 1 reverse transcription. Journal of Virology 70:4017–4027
    [Google Scholar]
  17. Hasegawa S. L., Doetsch P. W., Hamilton K. K., Martin A. M., Okenquist S. A., Lenz J., Boss J. M. 1991; DNA binding properties of YB-1 and dbpA: binding to double-stranded, single-stranded, and a basic site containing DNAs. Nucleic Acids Research 19:4915–4920
    [Google Scholar]
  18. Jones K. A., Kadonaga J. K., Luciw P. A., Tjian R. J. 1986; Activation of the AIDS retrovirus promoter by the cellular transcription factor SP1. Science 232:755–759
    [Google Scholar]
  19. Kashanchi F., Duvall J. F., Dittmer J., Mireskandari A., Reid R. L., Gitlin S. D., Brady J. N. 1994; Involvement of transcription factor YB-1 in human T cell lymphotropic virus type 1 basal gene expression. Journal of Virology 68:561–565
    [Google Scholar]
  20. Kerr D., Khalili K. 1991; A recombinant cDNA derived form human brain encodes a DNA binding protein that stimulates transcription of the human neurotropic virus JCV. Journal of Biological Chemistry 266:15876–15881
    [Google Scholar]
  21. Kingsman S. M., Kingsman A. J. 1996; The regulation of human immunodeficiency virus type 1 gene expression. European Journal of Biochemistry 240:491–507
    [Google Scholar]
  22. Kolluri R., Torrey T. A., Kinniburgh A. J. 1992; A CT promoter element binding protein: definition of a double-strand and a novel singlestrand DNA binding motif. Nucleic Acids Research 20:111–116
    [Google Scholar]
  23. Laughlin M. A., Chang G. Y., Oakes J. W., Gonzalez-Scarano F., Pomerantz R. J. 1995; Sodium butyrate stimulation of HIV-1 gene expression: a novel mechanism of induction independent of NF-kappa B. Journal of Acquired Immune Deficiency Syndrome and Human Retrovirology 9:332–339
    [Google Scholar]
  24. Liu Y. Z., Latchman D. S. 1997; The octamer-binding proteins Oct-1 and Oct-2 repress the HIV long terminal repeat promoter and its transactivation by Tat. Biochemical Journal 322:155–158
    [Google Scholar]
  25. MacDonald G. H., Itoh-Lindstrom Y., Ting J. P. 1995; The transcriptional regulatory protein, YB-1, promotes single-stranded regions in the DRA promoter. Journal of Biological Chemistry 270:3527–3533
    [Google Scholar]
  26. Majello B., DeLuca P., Hagen G. K., Suske G., Lania L. 1994; Different members of the Sp1 multigene family exert opposite transcriptional regulation of the long terminal repeat of HIV-1. Nucleic Acids Research 22:4914–4921
    [Google Scholar]
  27. Nabel G., Baltimore D. 1987; An inducible transactivation factor activates expression of human immunodeficiency virus in T cells. Nature 326:711–713 Erratum 344, 178; 1990
    [Google Scholar]
  28. Poli G., Fauci A. S. 1992; The effect of cytokines and pharmacologic agents on chronic HIV infection. AIDS Research and Human Retroviruses 8:191–197
    [Google Scholar]
  29. Rahman A., Esmaili A., Saatcioglu F. 1995; A unique thyroid hormone response element in the human immunodeficiency virus type 1 long terminal repeat that overlaps the Sp1 binding sites. Journal of Biological Chemistry 270:31059–31064
    [Google Scholar]
  30. Roulson A., Lin R., Beauparlant P., Wainberg M. A., Hiscott J. 1995; Regulat ion of human immunodeficiency virus type 1 and cytokine gene expression in myeloid cells by NF-kappa B/Rel tran-scription factors. Microbiological Reviews 59:481–505
    [Google Scholar]
  31. Sawaya B. E., Rohr O., Aunis D., Schaeffer E. 1996; Regulation of human immunodeficiency virus type 1 gene transcription by nuclear receptors in human brain cells. Journal of Biological Chemistry 271:22895–22900
    [Google Scholar]
  32. Taylor J. P., Khalili K. 1994; Activation of HIV-1 transcription by Tat in cells derived from the CNS: evidence for the participation of NF-kappa B - a review. Advances in Neuroimmunology 4:291–303
    [Google Scholar]
  33. Taylor J. P., Pomerantz R. J., Bagasra O., Chowdhury M., Rappaport J., Khalili K., Amini S. 1992a; TAR independent transactivation by Tat in cells derived from the CNS: a novel mechanism of HIV-1 gene regulation. EMBO Journal 11:3395–3403
    [Google Scholar]
  34. Taylor J. P., Cupp C., Diaz A., Chowdhury M., Khalili K., Jimenez S. A., Amini S. 1992b; Activation of expression of genes coding for extracellular matrix proteins in Tat producing glioblastoma cells. Proceedings of the National Academy of Sciences, USA 89:9617–9621
    [Google Scholar]
  35. Taylor J. P., Kundu M., Khalili K. 1993; TAR-independent activation of HIV-1 requires the activation domain but not the RNA-binding domain of Tat. Virology 195:780–785
    [Google Scholar]
  36. Taylor J. P., Pomerantz R. J., Raj G. V., Kashanchi F., Brady J. N., Amini S., Khalili K. 1994; Central nervous system derived cells express a kB-binding activity that enhances human immunodeficiency virus type 1 transcription in vitro and facilitates TAR-independent transactivation by Tat. Journal of Virology 68:3971–3981
    [Google Scholar]
  37. Valerie K., Singhal A., Kirkham J. C., Laster W. S., Rosenberg M. 1995; Activation of human immunodeficiency virus gene expression by ultraviolet light in stably transfected human cells does not require the enhancer element. Biochemistry 34:15760–15767
    [Google Scholar]
  38. Wang L., Mukherjee S., Jia F., Narayan O., Zhao L. J. 1995; Interaction of virion protein Vpr of human immunodeficiency virus type 1 with cellular transcription factor Sp1 and transactivation of viral long terminal repeat. Journal of Biological Chemistry 270:25564–25569
    [Google Scholar]
  39. Wolffe A. P. 1994; Structural and functional properties of the evolutionarily ancient Y-box family of nucleic acid binding proteins. Bioessays 16:245–251
    [Google Scholar]
  40. Yan C., Tamm I. 1991; Molecular cloning and characterization of interferon alpha/beta response element binding factors of the murine (2′-5′)oligoadenylate synthetase ME-12 gene. Proceedings of the National Academy of Sciences, USA 88:144–148
    [Google Scholar]
  41. Zeichner S. L., Kim J. Y., Alwine J. C. 1991; Linker-scanning mutational analysis of the transcriptional activity of the human immunodeficiency virus type 1 long terminal repeat. Journal of Virology 65:2436–2444
    [Google Scholar]
  42. Zhang L., Huang Y., Yuan H., Chen B. K., Ip J., Ho D. D. 1997; Identification of a replication-competent pathogenic human immunodeficiency virus type 1 with a duplication in the TCF-1 alpha region but lacking NF-kappa B binding sites. Journal of Virology 71:1651–1656
    [Google Scholar]
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