RT Journal Article SR Electronic(1) A1 Soo, H. M A1 Handley, J. A A1 Maugeri, M. M A1 Burns, P A1 Smith, G. R A1 Dale, J. L A1 Harding, R. MYR 1998 T1 Molecular characterization of Fiji disease fijivirus genome segment 9. JF Journal of General Virology, VO 79 IS 12 SP 3155 OP 3161 DO https://doi.org/10.1099/0022-1317-79-12-3155 PB Microbiology Society, SN 1465-2099, AB This is the first report of sequence from Fiji disease fijivirus (FDV), the type member of the genus Fijivirus of the family Reovi ridae. FDV genome segment (S9) comprised 1843 nt and contained two non-overlapping ORFs, separated by a 57 nt intergenic region. S9 ORF 1 comprised 1008 ntand encoded a 335-amino-acid polypeptide (predicted molecular mass 38·6 kDa), while ORF 2 comprised 627 nt and encoded a 208-amino-acid polypeptide (predicted molecular mass 23·8 kDa). The 5′ and 3′ non-coding regions were 49 and 102 nt, respectively. The S9 terminal sequences were 5′ AAGU-UUUU------UGUC 3′, and located immediately adjacent to these sequences were 12 bp imperfect inverted repeats. The entire S9 ORF 1 and the hydrophilic regions of S9 ORF 2 were each expressed as a fusion protein with the maltose-binding protein in Escherichia coli. Antibodies produced against the ORF 1 fusion protein reacted strongly with a protein of approximately 39 kDa present in both crude extracts of FDV-infected sugarcane and partially purified FDV preparations. In contrast, antibodies raised against the modified ORF 2 fusion protein did not react with any proteins in the same samples. Further, polyclonal antibodies produced against partially purified FDV reacted with the ORF 1, but not the modified ORF 2, fusion protein. These results indicate that FDV S9 ORF 1 encodes a major structural protein, while ORF 2 probably encodes a non-structural protein., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-12-3155