1887

Abstract

The potato A potyvirus (PVA)-encoded proteins P1, HC-Pro, P3, CI, VPg, NIaPro, NIb and coat protein (CP) were expressed as 6 × His-tagged recombinant proteins in and purified to homogeneity. RNA binding was tested using purified proteins in Northwestern and liquid assays. PVA proteins except P3 bound to positive- and negativesense transcripts prepared from the nontranslated 5′ - and 3′ -regions of PVA genomic RNA and to full- length transcripts of PVA RNA. RNA binding by these proteins showed no sequence specificity since they also bound to various non-PVA control RNAs. Binding properties of P1, HC-Pro, CI and NIaPro are consistent with previous studies carried out on a few other potyviruses, but the binding of VPg, NIb and CP to RNA reveal novel interactions between RNA and potyvirus proteins. Furthermore, the RNA- binding properties of all major proteins of a potyvirus have not been reported previously.

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/content/journal/jgv/10.1099/0022-1317-79-12-3123
1998-12-01
2022-05-28
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