1887

Abstract

Mutations of K → E in the highly conserved ‘KITC’ motif of the potyvirus helper component (HC) protein result in loss of HC function in aphid transmission, presumably because of inability to interact with virions, stylets or both. In this study we show that HC of potato virus C (PVC), a naturally occurring variant of potato virus Y (PVY) that has the K → E mutation, lacks the ability to be retained in stylets, whereas PVY HC is retained. The K → E mutation in either PVC or a site-directed mutant of tobacco etch virus (TEV) did not hinder binding to capsid protein, nor did deletion of the N-terminal 107 aa of TEV HC. An additional mutation, F → L at aa 10 of TEV HC, which renders HC non-functional but does not affect binding to capsid protein, is reported. Collectively, the results suggest that the N-terminal domain is required for interaction of HC with stylets rather than for binding to virions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-12-3119
1998-12-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/12/9880030.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-12-3119&mimeType=html&fmt=ahah

References

  1. Ammar E. D., Järlfors U., Pirone T. P. 1994; Association of potyvirus helper component protein with virions and the cuticle lining the maxillary food canal and foregut of an aphid vector. Phytopathology 84:1054–1060
    [Google Scholar]
  2. Atreya C. D., Pirone T. P. 1993; Mutational analysis of the helper component-proteinase gene of a potyvirus: effects of amino acid substitutions, deletions, and gene replacement on virulence and aphid transmissibility. Proceedings of the National Academy of Sciences, USA 90:11919–11923
    [Google Scholar]
  3. Atreya C. D., Atreya P. L., Thornbury D. W., Pirone T. P. 1992; Site-directed mutations in the potyvirus HC-PRO gene affect helper component activity, virus accumulation, and symptom expression in infected tobacco plants. Virology 191:106–111
    [Google Scholar]
  4. Berger P. H., Pirone T. P. 1986; The effect of helper component on the uptake and localization of potyviruses in Myzus persicae . Virology 153:256–261
    [Google Scholar]
  5. Blanc S., López-Moya J. J., Wang R. Y., García-Lampasona S., Thornbury D. W., Pirone T. P. 1997; A specific interaction between coat protein and helper component correlates with aphid transmission of a potyvirus. Virology 231:141–147
    [Google Scholar]
  6. Blanc S., Dolja V. V., Llave C., Pirone T. P. 1998; Histidinetagging and purification of tobacco etch potyvirus helper component protein. Journal of Virological Methods in press
    [Google Scholar]
  7. Canto T., Lopez-Moya J. J., Lopez-Abella D., Serra-Yoldi M. T., Diaz-Ruiz J. R. 1995; Different helper component mutations associated with lack of aphid transmissibility in two isolates of potato virus Y. Phytopathology 85:1519–1524
    [Google Scholar]
  8. Dolja V. V., Hong J., Keller K. E., Martin R. R., Peremyslov V. V. 1997; Suppression of potyvirus infection by co-expressed closterovirus protein. Virology 234:243–252
    [Google Scholar]
  9. Govier D. A., Kassanis B. 1974; A virus-induced component of plant sap needed when aphids acquire potato virus Y from purified preparations. Virology 61:420–426
    [Google Scholar]
  10. Govier D. A., Kassanis B., Pirone T. P. 1977; Partial purification and characterization of the potato virus Y helper component. Virology 78:306–314
    [Google Scholar]
  11. Granier F., Durand-Tardif M., Casse-Delbart F., Lecoq H., Robaglia C. 1993; Mutations in zucchini yellow mosaic virus helper component protein associated with loss of aphid transmissibility. Journal of General Virology 74:2737–2742
    [Google Scholar]
  12. Huet H., Gal-on A., Meir E., Lecoq H., Raccah B. 1994; Mutations in the helper component protease gene of zucchini yellow mosaic virus affect its ability to mediate aphid transmissibility. Journal of General Virology 75:1407–1414
    [Google Scholar]
  13. Nakashima H., Sako N., Hori K. 1993; Nucleotide sequences of the helper component-proteinase genes of aphid transmissible and nontransmissible isolates of turnip mosaic virus. Archives of Virology 131:17–27
    [Google Scholar]
  14. Peng Y, Gal-On A., Huet H., Wang Y., Raccah B. 1998; Mutations in the HC-Pro gene of zucchini yellow mosaic potyvirus: effects on aphid transmission and binding to purified virions. Journal of General Virology 79:897–904
    [Google Scholar]
  15. Thornbury D. W., Hellmann G. M., Rhoads R. E., Pirone T. P. 1985; Purification and characterization of potyvirus helper component. Virology 144:260–267
    [Google Scholar]
  16. Thornbury D. W., Patterson C. A., Dessens J. T., Pirone T. P. 1990; Comparative sequence of the helper component (HC) region of potato virus Y and a HC-defective strain, potato virus C. Virology 178:573–578
    [Google Scholar]
  17. Wang R. Y., Ammar E. D., Thornbury D. W., Lopez-Moya J. J., Pirone T. P. 1996; Loss of potyvirus transmissibility and helper component activity correlates with non-retention of virions in aphid stylets. Journal of General Virology 77:861–867
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-12-3119
Loading
/content/journal/jgv/10.1099/0022-1317-79-12-3119
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error