1887

Abstract

Four further circular ssDNA components (C7–C10), about 1 kb in size and structurally similar to the previously described components (C1–C6) found associated with a Syrian (Sy) isolate of faba bean necrotic yellows virus (FBNYV), have been identified. Similar to C1 and C2, two of the new components (C7 and C9) encode putative replication- associated (Rep) proteins of 33 ·2 and 32·7 kDa, respectively, the former of which is 90% identical to the C10 Rep protein of milk vetch dwarf virus (MDV). C8 encodes a putative protein (17 4 kDa) whose function is unknown, but which is highly conserved between FBNYV and the other nanoviruses MDV, subterranean clover stunt virus and banana bunchy top virus. The putative protein (19·7 kDa) encoded by C10 contains an LXCXE motif, which is also present in the homologues of its relatives, suggesting that they may all interact with plant retinoblastoma-like proteins. Sequence information for seven components of an Egyptian (Eg) FBNYV isolate indicated that six of them share > 96% identity with FBNYV-Sy. However, the C1 Rep protein of FBNYV-Eg was only 63·5% identical to that of FBNYV-Sy, but was 88·3 % identical to the MDV-C2 Rep protein. We conclude that the FBNYV genome consists of seven to ten components, six of which encode non-Rep proteins. All ten components of the FBNYV genome, except the Rep components C2 and C9, had closely related counterparts in the MDV genome. In spite of this similarity, FBNYV and MDV appear to be distinct virus species.

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1998-12-01
2022-05-21
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References

  1. Beetham P. R., Hafner G. J., Harding R. M., Dale J. L. 1997; Two mRNAs are transcribed from banana bunchy top virus DNA-1. Journal of General Virology 78:229–236
    [Google Scholar]
  2. Bisaro D. M. 1996; Geminivirus DNA replication. In DNA Replication in Eukaryotic Cells pp. 833–854 DePamphilis M. L. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  3. Boevink P., Chu P. W., Keese P. 1995; Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses. Virology 207:354–361
    [Google Scholar]
  4. Burns T. M., Harding R. M., Dale J. L. 1995; The genome organization of banana bunchy top virus : analysis of six ssDNA components. Journal of General Virology 76:1471–1482
    [Google Scholar]
  5. Choi I.-R., Stenger D. C. 1995; Strain-specific determinants of beet curly top geminivirus DNA replication. Virology 206:904–912
    [Google Scholar]
  6. Chu P. W. G., Helms K. 1988; Novel virus-like particles containing circular single-stranded DNA associated with subterranean clover stunt disease. Virology 167:38–49
    [Google Scholar]
  7. Chu P. W., Keese P., Qiu B. S., Waterhouse P. M., Gerlach W. L. 1993; Putative full-length clones of the genomic DNA segments of subterranean clover stunt virus and identification of the segment coding for the viral coat protein. Virus Research 27:161–171
    [Google Scholar]
  8. Desbiez C., David C., Mettouchi A., Laufs J., Gronenborn B. 1995; Rep protein of tomato yellow leaf curl geminivirus has an ATPase activity required for viral DNA replication. Proceedings of the National Academy of Sciences, USA 92:5640–5644
    [Google Scholar]
  9. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  10. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using bootstrap. Evolution 39:783–791
    [Google Scholar]
  11. Franz A. 1997 Diagnosis and biological characterization of faba bean necrotic yellows virus with emphasis on its epidemiology in countries of west Asia and north Africa PhD thesis University of Kiel, Germany (in German):
    [Google Scholar]
  12. Franz A., Makkouk K. M., Vetten H. J. 1995; Faba bean necrotic yellows virus naturally infects Phaseolus bean and cowpea in the coastal area of Syria. Journal of Phytopathology 143:319–320
    [Google Scholar]
  13. Franz A., Makkouk K. M., Katul L., Vetten H. J. 1996; Monoclonal antibodies for the detection and differentiation of faba bean necrotic yellows virus isolates. Annals of Applied Biology 128:255–268
    [Google Scholar]
  14. Franz A., Makkouk K. M., Vetten H. J. 1997; Host range of faba bean necrotic yellows virus and potential yield loss in infected faba bean. Phytopathologia Mediterranea 36:94–103
    [Google Scholar]
  15. Franz A., Makkouk K. M., Vetten H. J. 1998; Acquisition, retention and transmission of faba bean necrotic yellows virus by two of its aphid vectors, Aphis craccivora (Koch) and Acyrthosiphon pisum (Harris). Journal of Phytopathology 146:347–355
    [Google Scholar]
  16. Hafner G. J., Stafford M. R., Wolter L. C., Harding R. M., Dale J. L. 1997; Nicking and joining activity of banana bunchy top virus replication protein in vitro . Journal of General Virology 78:1795–1799
    [Google Scholar]
  17. Harding R. M., Burns T. M., Dale J. L. 1991; Virus-like particles associated with banana bunchy top disease contain small single-stranded DNA. Journal of General Virology 72:225–230
    [Google Scholar]
  18. Harding R. M., Burns T. M., Hafner G., Dietzgen R. G., Dale J. L. 1993; Nucleotide sequence of one component of the banana bunchy top virus genome contains a putative replicase gene. Journal of General Virology 74:323–328
    [Google Scholar]
  19. Heyraud-Nitschke F., Schumacher S., Laufs J., Schaefer S., Schell J., Gronenborn B. 1995; Determination of the origin cleavage and joining domain of geminivirus Rep proteins. Nucleic Acids Research 23:910–916
    [Google Scholar]
  20. Higgins D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment. Computer Applications in the Biosciences 8:189–191
    [Google Scholar]
  21. Horser C., Karan M., Harding R. M., Dale J. L. 1996; Possible satellite DNAs associated with banana bunchy top virus. Xth International Congress of Virology Jerusalem: Abstract PW52-2 p. 237
    [Google Scholar]
  22. Inouye T., Inouye N., Mitsuhata K. 1968; Yellow dwarf ofpea and broad bean caused by milk-vetch dwarf virus. Annals of the Phytopathological Society of Japan 34:28–35 in Japanese with English abstract
    [Google Scholar]
  23. Jupin I., Hericourt F., Benz B., Gronenborn B. 1995; DNA replication specificity of TYLCV geminivirus is mediated by the amino- terminal 116 amino acids of the Rep protein. FEBS Letters 362:116–120
    [Google Scholar]
  24. Karan M., Harding R. M., Dale J. L. 1994; Evidence for two groups of banana bunchy top virus isolates. Journal of General Virology 75:3541–3546
    [Google Scholar]
  25. Katul L., Vetten H. J., Maiss E., Makkouk K. M., Lesemann D.-E., Casper R. 1993; Characterisation and serology of virus-like particles associated with faba bean necrotic yellows. Annals of Applied Biology 123:629–647
    [Google Scholar]
  26. Katul L., Maiss E., Vetten H. J. 1995; Sequence analysis of a faba bean necrotic yellows virus DNA component containing a putative replicase gene. Journal of General Virology 76:475–479
    [Google Scholar]
  27. Katul L., Maiss E., Morozov S. Y., Vetten H. J. 1997; Analysis of six DNA components of the faba bean necrotic yellows virus genome and their structural affinity to related plant virus genomes. Virology 233:247–259
    [Google Scholar]
  28. Koonin E. V., Ilyina T. V. 1992; Geminivirus replication proteins are related to prokaryotic plasmid rolling circle DNA replication initiator proteins. Journal of General Virology 73:2763–2766
    [Google Scholar]
  29. Laufs J., Jupin I., David C., Schumacher S., Heyraud-Nitschke F., Gronenborn B. 1995a; Geminivirus replication: genetic and biochemical characterization of Rep protein function, a review. Biochimie 77:765–773
    [Google Scholar]
  30. Laufs J., Schumacher S., Geisler N., Jupin I., Gronenborn B. 1995b; Identification of the nicking tyrosine of geminivirus Rep protein. FEBS Letters 377:258–262
    [Google Scholar]
  31. Laufs J., Traut W., Heyraud F., Matzeit V., Rogers S. G., Schell J., Gronenborn B. 1995c; In vitro cleavage and joining at the viral origin of replication by the replication initiator protein of tomato yellow leaf curl virus. Proceedings of the National Academy of Sciences, USA 92:3879–3883
    [Google Scholar]
  32. Lazarowitz S. G. 1992; Geminiviruses: genome structure and gene function. CRC Critical Reviews in Plant Sciences 11:327–349
    [Google Scholar]
  33. Makkouk K. M., Kumari S. G., Al-Daoud R. 1992; Survey of viruses affecting lentil (Lens culinaris Med.) in Syria. Phytopathologia Mediterranea 31:188–190
    [Google Scholar]
  34. Makkouk K. M., Rizkallah L., Madkour M., El-Sherbeeny M., Kumari S. G., Amriti A. W., Solh M. B. 1994; Survey of faba bean (Vicia faba L.) for viruses in Egypt. Phytopathologia Mediterranea 33:207–211
    [Google Scholar]
  35. Noris E., Accotto G. P., Tavazza R., Brunetti A., Crespi S., Tavazza M. 1996; Resistance to tomato yellow leaf curl geminivirus in Nicotiana benthamiana transformed with a truncated viral C1 gene. Virology 224:130–138
    [Google Scholar]
  36. Padidam M., Beachy R. N., Fauquet C. M. 1995; Classification and identification of geminiviruses using sequence comparisons. Journal of General Virology 76:249–263
    [Google Scholar]
  37. Rohde W., Randles J. W., Langridge P., Hanold D. 1990; Nucleotide sequence of a circular single-stranded DNA associated with coconut foliar decay virus. Virology 176:648–651
    [Google Scholar]
  38. Rybicki E. P. 1994; A phylogenetic and evolutionary justification for three genera of Geminiviridae. Archives of Virology 139:49–77
    [Google Scholar]
  39. Saitou N., Nei M. 1987; The neighbor-joining method : a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425
    [Google Scholar]
  40. Sano Y., Isogai M., Satoh S., Kojima M. 1993; Small virus-like particles containing single-stranded DNAs associated with milk-vetch dwarf disease in Japan. 6th International Congress of Plant Pathology Montreal: Abstract no. 17.1. 27 p. 305
    [Google Scholar]
  41. Sano Y., Wada M., Hashimoto Y., Matsumoto T., Kojima M. 1998; Sequences of ten circular ssDNA components associated with the milk vetch dwarf virus genome. Journal of General Virology 79:3111–3118
    [Google Scholar]
  42. Thomas J. E., Dietzgen R. G. 1991; Purification, characterization and serological detection of virus-like particles associated with banana bunchy top disease in Australia. Journal of General Virology 72:217–224
    [Google Scholar]
  43. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
    [Google Scholar]
  44. Wanitchakorn R., Harding R. M., Dale J. L. 1997; Banana bunchy top virus DNA-3 encodes the viral coat protein. Archives of Virology 142:1673–1680
    [Google Scholar]
  45. Weinberg R. A. 1995; The retinoblastoma protein and cell cycle control. Cell 81:323–330
    [Google Scholar]
  46. Wu R. Y., You L. R., Soong T. S. 1994; Nucleotide sequences of two circular single-stranded DNAs associated with banana bunchy top virus. Phytopathology 84:952–958
    [Google Scholar]
  47. Xie Q., Suárez-López P., Guitiérrez C. 1995; Identification and analysis of a retinoblastoma binding motif in the replication protein of a plant DNA virus: requirement for efficient viral DNA replication. EMBO Journal 14:4073–4082
    [Google Scholar]
  48. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains : nucleotide sequences of the Ml3mp18 and pUCl9 vectors. Gene 33:103–119
    [Google Scholar]
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