1887

Abstract

Chicken anaemia virus (CAV) expresses three proteins, VP1, VP2 and VP3, but its capsid contains only the VP1 protein. In this paper, we report that for production of the neutralizing epitope, cosynthesis of (recombinant) VP1 and VP2 has to take place. We show via immunofluorescence that recom- binant-baculovirus-infected Sf9 cells synthesizing VP1 (or VP2) alone react very poorly with CAV- specific neutralizing antibodies. In contrast, Sf9 cells co-infected with VP1- and VP2-recombinant baculo-viruses, or infected with a single recombinant baculovirus co-expressing both VP1 and VP2, react strongly with the neutralizing antibodies. Furthermore, immunoprecipitation assays show that VP1 and VP2 interact directly with each other, which indicates that the non-structural protein VP2 might act as a scaffold protein in virion assembly. Recombinant baculovirus expressing VP1 and VP2 is, therefore, a potential production system for a subunit vaccine against CAV infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-12-3073
1998-12-01
2022-05-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/12/9880024.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-12-3073&mimeType=html&fmt=ahah

References

  1. D’Halluin J. C., Martin G. R., Torpier G., Boulanger P. A. 1978; Adenovirus type 2 assembly analyzed by reversible cross-linking of labile intermediates. Journal of Virology 26:357–363
    [Google Scholar]
  2. Douglas A. J., Phenix K., Mawhinney K. A., Todd D., Mackie D. P., Curran W. L. 1995; Identification of a 24 kDa protein expressed by chicken anaemia virus. Journal of General Virology 76:1557–1562
    [Google Scholar]
  3. Gelderblom H., Kling S., Lurz R., Tischer I., VonBulow V. 1989; Morphological characterization of chicken anaemia agent. Journal of General Virology 71:819–823
    [Google Scholar]
  4. Graham F. L., Van der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 52:456–467
    [Google Scholar]
  5. Gustin K. E., Lutz P., Imperiale M. J. 1996; Interaction of the adenovirus L1 52/55-kilodalton protein with the IVa2 gene product during infection. Journal of Virology 70:6463–6467
    [Google Scholar]
  6. Hoeben R. C., Van der Jagt R. C. M., Schoute F., Van Tilburg N. H., Verbeet M. Ph., Briet E., Van Ormondt H., Van der Eb A. J. 1990; Expression of functional factor VIII in primary human skin fibroblasts after retrovirus-mediated gene transfer. Journal of Biological Chemistry 265:7318–7323
    [Google Scholar]
  7. Jeurissen S. H. M., Wagenaar F., Pol J. M. A., VanderEb A. J., Noteborn M. H. M. 1992; Chicken anemia virus causes apoptosis of thymocytes after in vivo infection and of cell lines after in vitro infection. Journal of Virology 66:7383–7388
    [Google Scholar]
  8. Koch G., VanRoozelaar D. J., Verschueren C. A. J., VanderEb A. J., Noteborn M. H. M. 1995; Immunogenic and protective properties of chicken anaemia virus proteins expressed by baculovirus. Vaccine 13:763–770
    [Google Scholar]
  9. McIlroy S. G., McNulty M. S., Bruce D. W., Smyth J. A., Goodall E. A., Alcorn M. J. 1992; Economic effects of clinical chicken anemia agent infection on profitable broiler breeder flocks. Avian Diseases 36:566–574
    [Google Scholar]
  10. McNulty M. S. 1991; Chicken anemia agent : a review. Avian Pathology 20:187–203
    [Google Scholar]
  11. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Noteborn M. H. M., Koch G. 1995; Chicken anaemia virus infection: molecular basis of pathogenicity. Avian Pathology 24:11–31
    [Google Scholar]
  13. Noteborn M. H. M., DeBoer G. F., Kant A., Koch G., Bos J. L., Zantema A., VanderEb A. J. 1990; Expression of avian leukaemia virus env-gp85 inSpodoptera frugiperdacells by use of a baculovirus expression vector. Journal of General Virology 71:2641–2648
    [Google Scholar]
  14. Noteborn M. H. M., DeBoer G. F., VanRoozelaar D. J., Karreman C., Kranenburg O., Vos J. G., Jeurissen S. H. M., Hoeben R. C., Zantema A., Koch G., VanOrmondt H., VanderEb A. J. 1991; Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle. Journal of Virology 65:3131–3139
    [Google Scholar]
  15. Noteborn M. H. M., Kranenburg O., Zantema A., Koch G., DeBoer G. F., VanderEb A. J. 1992; Transcription of the chicken anaemia virus (CAV) genome and the synthesis of its 52-kDa protein. Gene 118:267–271
    [Google Scholar]
  16. Noteborn M. H. M., Todd D., Verschueren C. A. J., De Gauw H. W. F. M., Curran W. L., Veldkamp S., Douglas A. J., McNulty M. S., Van der Eb A. J., Koch G. 1994; A single chicken anemia virus protein induces apoptosis. Journal of Virology 68:346–351
    [Google Scholar]
  17. Persson H., Mathisen B., Philipson L., Petterson V. A. 1979; A maturation protein in adenovirus morphogenesis. Virology 93:198–208
    [Google Scholar]
  18. Phenix K. V., Meehan B. M., Philipson L., McNulty M. S. 1994; Transcriptional analysis and genome expression of chicken anemia virus. Journal of General Virology 75:905–909
    [Google Scholar]
  19. Smith G. E., Summers M. D., Fraser M. J. 1983; Production of human beta-interferon in insect cells infected with a baculovirus expression vector. Molecular and Cellular Biology 3:2156–2165
    [Google Scholar]
  20. Summers M. D., Smith G. E. 1987; A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Experimental State Bulletin1555
    [Google Scholar]
  21. Todd D., Creelan J. L., Mackie D. P., Rixon F., McNulty M. S. 1990; Purification and biochemical characterization of chicken anaemia agent. Journal of General Virology 71:819–823
    [Google Scholar]
  22. Trus B. L., Booy F. P., Newcomb W. W., Brown J. C., Homa F. L., Thomsen D. R., Steven A. C. 1996; The herpes simplex virus procapsid : structure, conformational changes upon maturation, and roles of the triplex proteins VP19c and VP23 in assembly. Journal of Molecular Biology 263:447–462
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-12-3073
Loading
/content/journal/jgv/10.1099/0022-1317-79-12-3073
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error