1887

Abstract

Human herpesvirus-8 (HHV-8) DNA sequences have been identified in all forms of Kaposi’s sarcoma (KS), a cancer found primarily in adult AIDS patients. We have identified HHV-8 strains in a rare human immunodeficiency virus (HlV)-negative form of KS, which is endemic in children in parts of sub-Saharan Africa. This was shown in Zambia, where we also had identified HHV-8 sequences in blood from HIVnegative febrile children without KS. In order to investigate the relationship of these Zambian strains to each other and to those from other forms of KS, we compared them to strains we have characterized from European AIDS KS (Denmark) and all published sequences from all forms of KS. Four distinct genomic regions were examined by PCR and sequencing: ORF26, ORF75, gH and K1. The results showed a distinct grouping of strains from both sets of Zambian children in all genomic regions studied, but which was most pronounced in the K1 glycoprotein gene. This gene was highly variable, encoding up to 25% amino acid sequence variation. In contrast, the Zambian groups were closely related to each other, with only 2% variation. Similar results were found in comparisons to the K1 sequences from HIV-positive febrile infants or KS children. The data raise the possibility that in areas where rare childhood endemic KS occurs, geographical variation in HHV-8 may relate to differences in virulence or transmission.

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1998-12-01
2022-08-15
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References

  1. Albrecht J. -C., Nicholas J., Biller D., Cameron K. R., Biesinger B., Newman C., Wittmann S., Craxton M. A., Coleman H., Fleckenstein B., Honess R. W. 1992; Primary structure of the herpesvirus saimiri genome. Journal of Virology 66:5047–5058
    [Google Scholar]
  2. Athale U. H., Patil P. S., Chintu C., Elem B. 1995; Influence of HIV epidemic on the incidence of Kaposi’s sarcoma in Zambian children. Journal of AIDS & Human Retrovirology 8:96–100
    [Google Scholar]
  3. Besnard V., Euvrard S., Kanitakis J., Mion F., Boillot O., Frances C., Faure M., Claudy A. 1996; Kaposi’s sarcoma after liver transplantation. Dermatology 193:100–104
    [Google Scholar]
  4. Cesarman E., Nador R. G., Aozasa K., Delsol G., Said J. W., Knowles D. M. 1996; Kaposi’s sarcoma-associated herpesvirus in non-AIDS-related lymphomas occurring in body cavities. American Journal of Pathology 149:53–57
    [Google Scholar]
  5. Chang Y., Cesarman E., Pessin M. S., Lee F., Culpepper J., Knowles D. M., Moore P. S. 1994; Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi’s sarcoma. Science 266:1865–1869
    [Google Scholar]
  6. Chintu C., Athale U. H., Patil P. S. 1995; Childhood cancers in Zambia before and after the HIV epidemic. ArchivesofDisease in Childhood 73:100–105
    [Google Scholar]
  7. Chuck S., Grant R. M., Katongolembidde E., Conant M., Ganem D. 1996; Frequent presence of a novel herpesvirus genome in lesions of human immunodeficiency virus-negative Kaposi’s sarcoma. Journal of Infectious Diseases 173:248–251
    [Google Scholar]
  8. Efstathiou S., Ho Y. M., Hall S., Styles C. J., Scott S. D., Gompels U. A. 1990; Murine herpesvirus 68 is genetically related to the gammaherpesviruses Epstein-Barr virus and herpesvirus saimiri. Journal of General Virology 71:1365–1372
    [Google Scholar]
  9. Ensser A., Pflanz R., Fleckenstein B. 1997; Primary structure of the alcelaphine herpesvirus 1 genome. Journalof Virology 71:6517–6525
    [Google Scholar]
  10. Gompels U. A., Craxton M. A., Honess R. W. 1988; Conservation of gene organization in the lymphotropic herpesviruses herpesvirus saimiri and Epstein-Barr virus. Journal of Virology 62:757–767
    [Google Scholar]
  11. Gompels U. A., Carrigan D. R., Carss A. L., Arno J. 1993; Two groups of human herpesvirus 6 identified by sequence analyses of laboratory strains and variants from Hodgkin’s lymphoma and bone marrow transplant patients. Journal of General Virology 74:613–622
    [Google Scholar]
  12. Gompels U. A., Nicholas J., Lawrence G., Jones M., Thomson B. J., Martin M., Efstathiou S., Craxton M., Macaulay H. A. 1995; The DNA sequence of human herpesvirus-6 structure, coding content, and genome evolution. Virology 209:29–51
    [Google Scholar]
  13. Higgins D. G., Sharp P. M. 1989; Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5:151–153
    [Google Scholar]
  14. Huang Y. Q., Li J. J., Kaplan M. H., Poiesz B., Katabira E., Zhang W. C., Feiner D., Friedmankien A. E. 1995; Human herpesvirus-like nucleic acid in various forms of Kaposi’s sarcoma. Lancet 345:759–761
    [Google Scholar]
  15. Jenkins P. J., Farrell P. J. 1996; . Are particular Epstein-Barr virus strains linked to disease? Seminars in Cancer Biology 7:209–215
    [Google Scholar]
  16. Kasolo F. C., Mpabalwani E., Gompels U. A. 1997; Infection with AIDS-related herpesviruses in human immunodeficiency virus-negative infants and endemic childhood Kaposi’s sarcoma in Africa. Journal of General Virology 78:847–856
    [Google Scholar]
  17. Kretschmer C., Murphy C., Biesinger B., Beckers J., Fickenscher H., Kirchner T., Fleckenstein B., Ruther U. 1996; A herpes saimiri oncogene causing peripheral T-cell lymphoma in transgenic mice. Oncogene 12:1609–1616
    [Google Scholar]
  18. Lagunoff M., Ganem D. 1997; The structure and coding organization of the genomic termini of Kaposi’s sarcoma-associated herpesvirus (human herpesvirus 8). GenBank Accession U86667
    [Google Scholar]
  19. Lee H., Trimble J. J., Yoon D. W., Regier D., Desrosiers R. C., Jung J. U. 1997; Genetic variation of herpesvirus saimiri subgroup A transforming protein and its association with cellular src. Journal of Virology 71:3817–3825
    [Google Scholar]
  20. Lee H., Veazey R., Williams K., Li M., Guo J., Neipel F., Fleckenstein B., Lackner A., Desrosiers R. C., Jung J. U. 1998; Deregulation of cell growth by the K1 gene of Kaposi’s sarcoma-associated herpesvirus. Nature Medicine 4:3698–3704
    [Google Scholar]
  21. Lund T., Medveczky M. M., Medveczky P. G. 1997; Herpesvirus saimiri Tip-484 membrane protein markedly increases p56(lck) activity in T cells. Journal of Virology 71:378–382
    [Google Scholar]
  22. Luxton J. C., Rose R. C., Coletart T., Wilson P., Shepherd P. S. 1996; Serological and T-helper cell responses to human papillomavirus type 16 L1 in women with cervical dysplasia or cervical carcinoma and in healthy controls. Journal of General Virology 78:917–923
    [Google Scholar]
  23. Matondo P., Zumla A. 1996; The spectrum of African Kaposi’s sarcoma: is it consequential upon diverse immunological responses?. Scandinavian Journal of Infectious Diseases 28:225–230
    [Google Scholar]
  24. Moore P. S., Gao S. J., Dominguez G., Cesarman E., Lungu O., Knowles D. M., Garber R., Pellett P. E., McGeoch D. J., Chang Y. 1996; Primary characterization of a herpesvirus agent associated with Kaposi’s sarcoma. Journal of Virology 70:549–558
    [Google Scholar]
  25. Nanteza M., Kasolo F. C., Monze M., Gompels U. A. 1998; Detecting ‘undetected’ HIV-1 variants in African children using degenerate polymerase chain reaction and sequence analysis. Transactions of the Royal Society of Tropical Medicine and Hygiene 92:294–295
    [Google Scholar]
  26. Neipel F., Albrecht J. -C., Ensser A., Huang Y. -Q., Li J. J., FriedmanKien A. E., Fleckenstein B. 1997a; The genome of human herpesvirus 8 cloned from Kaposi’s sarcoma. GenBank Accession U93872
    [Google Scholar]
  27. Neipel F., Albrecht J. -C., Fleckenstein B. 1997b; Cell-homologous genes in the Kaposi’s sarcoma-associated rhadinovirus human herpesvirus 8: determinants of its pathogenicity?. Journal of Virology 71:4187–4192
    [Google Scholar]
  28. Nicholas J., Zong J. -C., Alcendor D. J., Ciufo D. M., Poole L. J., Sarisky R. T., Chiou C. -J., Zhang X., Wan X., Guo H. -G., Reitz M., SHayward G. S. 1998; Novel organizational features, captured cellular genes, and strain variability within the genome of KSHV/HHV8. Journal of the National Cancer Institute Monographs 23:79–88
    [Google Scholar]
  29. Niewiesk S., Bangham C. 1996; Evolution in a chronic RNA virus infection: selection on HTLV-I tax protein differs between healthy carriers and patients with tropical spastic paraparesis. Journal of Molecular Evolution 42:452–458
    [Google Scholar]
  30. Russo J. J., Bohenzky R. A., Chien M. -C., Chen J., Yan M., Maddalena D., Parry J. P., Peruzzi D., Edelman I.S., Chang Y., Moore P. S. 1996; Nucleotide sequence of Kaposi’s sarcoma-associated herpesvirus (HHV-8). Proceedings of the National Academy of Sciences, USA 93:14862–14868
    [Google Scholar]
  31. Sample J., Young L., Martin B., Chatman T., Kieff E., Rickinson A., Kieff E. 1990; Epstein-Barr virus types 1 and 2 differ in their EBNA-3A, EBNA-3B, and EBNA-3C genes. Journal of Virology 64:4084–4092
    [Google Scholar]
  32. Soulier J., Grollet L., Oksenhendler E., Cacoub P., Cazalshatem D., Babinet P., Dagay M. F., Clauvel J. P., Raphael M., Degos L., Sigaux F. 1995; Kaposi’s sarcoma-associated herpesvirus-like DNA sequences in multicentric Castleman’s disease. Blood 86:1276–1280
    [Google Scholar]
  33. Sun Y. 1995; Molecular oncology of human nasopharyngeal carcinoma. Cancer Journal 8:325–330
    [Google Scholar]
  34. Zong J. C., Metroka C., Reitz M. S., Nicholas J., Hayward G. S. 1997; Strain variability among Kaposi sarcoma-associated herpesvirus (human herpesvirus 8) genomes: evidence that a large cohort of United States AIDS patients may have been infected by a single common isolate. Journal of Virology 71:2505–2511
    [Google Scholar]
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